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Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface

The spontaneous healing of a tendon laceration results in the formation of scar tissue, which has lower functionality than the original tissue. Moreover, chronic non-healing tendon injuries frequently require surgical treatment. Several types of scaffolds have been developed using the tissue enginee...

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Autores principales: Faccendini, Angela, Bianchi, Eleonora, Ruggeri, Marco, Vigani, Barbara, Perotti, Cesare, Pavesi, Francesco Claudio, Caliogna, Laura, Natali, Francesca, Del Favero, Elena, Cantu’, Laura, Ferrari, Franca, Rossi, Silvia, Sandri, Giuseppina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707843/
https://www.ncbi.nlm.nih.gov/pubmed/34959280
http://dx.doi.org/10.3390/pharmaceutics13121996
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author Faccendini, Angela
Bianchi, Eleonora
Ruggeri, Marco
Vigani, Barbara
Perotti, Cesare
Pavesi, Francesco Claudio
Caliogna, Laura
Natali, Francesca
Del Favero, Elena
Cantu’, Laura
Ferrari, Franca
Rossi, Silvia
Sandri, Giuseppina
author_facet Faccendini, Angela
Bianchi, Eleonora
Ruggeri, Marco
Vigani, Barbara
Perotti, Cesare
Pavesi, Francesco Claudio
Caliogna, Laura
Natali, Francesca
Del Favero, Elena
Cantu’, Laura
Ferrari, Franca
Rossi, Silvia
Sandri, Giuseppina
author_sort Faccendini, Angela
collection PubMed
description The spontaneous healing of a tendon laceration results in the formation of scar tissue, which has lower functionality than the original tissue. Moreover, chronic non-healing tendon injuries frequently require surgical treatment. Several types of scaffolds have been developed using the tissue engineering approach, to complement surgical procedures and to enhance the healing process at the injured site. In this work, an electrospun hybrid tubular scaffold was designed to mimic tissue fibrous arrangement and extracellular matrix (ECM) composition, and to be extemporaneously loaded into the inner cavity with human platelet lysate (PL), with the aim of leading to complete post-surgery functional regeneration of the tissue for functional regeneration of the osteo–tendon interface. For this purpose, pullulan (P)/chitosan (CH) based polymer solutions were enriched with hydroxyapatite nanoparticles (HP) and electrospun. The nanofibers were collected vertically along the length of the scaffold to mimic the fascicle direction of the tendon tissue. The scaffold obtained showed tendon-like mechanical performance, depending on HP content and tube size. The PL proteins were able to cross the scaffold wall, and in vitro studies have demonstrated that tenocytes and osteoblasts are able to adhere to and proliferate onto the scaffold in the presence of PL; moreover, they were also able to produce either collagen or sialoproteins, respectively—important components of ECM. These results suggest that HP and PL have a synergic effect, endorsing PL-loaded HP-doped aligned tubular scaffolds as an effective strategy to support new tissue formation in tendon-to-bone interface regeneration.
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spelling pubmed-87078432021-12-25 Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface Faccendini, Angela Bianchi, Eleonora Ruggeri, Marco Vigani, Barbara Perotti, Cesare Pavesi, Francesco Claudio Caliogna, Laura Natali, Francesca Del Favero, Elena Cantu’, Laura Ferrari, Franca Rossi, Silvia Sandri, Giuseppina Pharmaceutics Article The spontaneous healing of a tendon laceration results in the formation of scar tissue, which has lower functionality than the original tissue. Moreover, chronic non-healing tendon injuries frequently require surgical treatment. Several types of scaffolds have been developed using the tissue engineering approach, to complement surgical procedures and to enhance the healing process at the injured site. In this work, an electrospun hybrid tubular scaffold was designed to mimic tissue fibrous arrangement and extracellular matrix (ECM) composition, and to be extemporaneously loaded into the inner cavity with human platelet lysate (PL), with the aim of leading to complete post-surgery functional regeneration of the tissue for functional regeneration of the osteo–tendon interface. For this purpose, pullulan (P)/chitosan (CH) based polymer solutions were enriched with hydroxyapatite nanoparticles (HP) and electrospun. The nanofibers were collected vertically along the length of the scaffold to mimic the fascicle direction of the tendon tissue. The scaffold obtained showed tendon-like mechanical performance, depending on HP content and tube size. The PL proteins were able to cross the scaffold wall, and in vitro studies have demonstrated that tenocytes and osteoblasts are able to adhere to and proliferate onto the scaffold in the presence of PL; moreover, they were also able to produce either collagen or sialoproteins, respectively—important components of ECM. These results suggest that HP and PL have a synergic effect, endorsing PL-loaded HP-doped aligned tubular scaffolds as an effective strategy to support new tissue formation in tendon-to-bone interface regeneration. MDPI 2021-11-24 /pmc/articles/PMC8707843/ /pubmed/34959280 http://dx.doi.org/10.3390/pharmaceutics13121996 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Faccendini, Angela
Bianchi, Eleonora
Ruggeri, Marco
Vigani, Barbara
Perotti, Cesare
Pavesi, Francesco Claudio
Caliogna, Laura
Natali, Francesca
Del Favero, Elena
Cantu’, Laura
Ferrari, Franca
Rossi, Silvia
Sandri, Giuseppina
Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface
title Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface
title_full Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface
title_fullStr Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface
title_full_unstemmed Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface
title_short Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface
title_sort smart device for biologically enhanced functional regeneration of osteo–tendon interface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707843/
https://www.ncbi.nlm.nih.gov/pubmed/34959280
http://dx.doi.org/10.3390/pharmaceutics13121996
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