Cargando…
Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus
Feline coronavirus (FCoV) is a pathogenic virus commonly found in cats that causes a benign enteric illness and fatal systemic disease, feline infectious peritonitis. The development of serological diagnostic tools for FCoV is helpful for clinical diagnosis and epidemiological investigation. Therefo...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707903/ https://www.ncbi.nlm.nih.gov/pubmed/34960764 http://dx.doi.org/10.3390/v13122496 |
_version_ | 1784622552023302144 |
---|---|
author | Dong, Bo Zhang, Gaoqiang Zhang, Xiaodong Chen, Xufei Zhang, Meiling Li, Linglin Lin, Weiming |
author_facet | Dong, Bo Zhang, Gaoqiang Zhang, Xiaodong Chen, Xufei Zhang, Meiling Li, Linglin Lin, Weiming |
author_sort | Dong, Bo |
collection | PubMed |
description | Feline coronavirus (FCoV) is a pathogenic virus commonly found in cats that causes a benign enteric illness and fatal systemic disease, feline infectious peritonitis. The development of serological diagnostic tools for FCoV is helpful for clinical diagnosis and epidemiological investigation. Therefore, this study aimed to develop an indirect enzyme-linked immunosorbent assay (iELISA) to detect antibodies against FCoV using histidine-tagged recombinant spike protein. FCoV S protein (1127–1400 aa) was expressed and used as an antigen to establish an ELISA. Mice and rabbits immunized with the protein produced antibodies that were recognized and bound to the protein. The intra-assay coefficient of variation (CV) was 1.15–5.04% and the inter-assay CV was 4.28–15.13%, suggesting an acceptable repeatability. iELISA did not cross-react with antisera against other feline viruses. The receiver operating characteristic curve analysis revealed an 86.7% sensitivity and 93.3% specificity for iELISA. Serum samples (n = 107) were tested for anti-FCoV antibodies, and 70.09% of samples were positive for antibodies against FCoV. The iELISA developed in our study can be used to measure serum FCoV antibodies due to its acceptable repeatability, sensitivity, and specificity. Additionally, field sample analysis data demonstrated that FCoV is highly prevalent in cat populations in Fujian province, China. |
format | Online Article Text |
id | pubmed-8707903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87079032021-12-25 Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus Dong, Bo Zhang, Gaoqiang Zhang, Xiaodong Chen, Xufei Zhang, Meiling Li, Linglin Lin, Weiming Viruses Article Feline coronavirus (FCoV) is a pathogenic virus commonly found in cats that causes a benign enteric illness and fatal systemic disease, feline infectious peritonitis. The development of serological diagnostic tools for FCoV is helpful for clinical diagnosis and epidemiological investigation. Therefore, this study aimed to develop an indirect enzyme-linked immunosorbent assay (iELISA) to detect antibodies against FCoV using histidine-tagged recombinant spike protein. FCoV S protein (1127–1400 aa) was expressed and used as an antigen to establish an ELISA. Mice and rabbits immunized with the protein produced antibodies that were recognized and bound to the protein. The intra-assay coefficient of variation (CV) was 1.15–5.04% and the inter-assay CV was 4.28–15.13%, suggesting an acceptable repeatability. iELISA did not cross-react with antisera against other feline viruses. The receiver operating characteristic curve analysis revealed an 86.7% sensitivity and 93.3% specificity for iELISA. Serum samples (n = 107) were tested for anti-FCoV antibodies, and 70.09% of samples were positive for antibodies against FCoV. The iELISA developed in our study can be used to measure serum FCoV antibodies due to its acceptable repeatability, sensitivity, and specificity. Additionally, field sample analysis data demonstrated that FCoV is highly prevalent in cat populations in Fujian province, China. MDPI 2021-12-13 /pmc/articles/PMC8707903/ /pubmed/34960764 http://dx.doi.org/10.3390/v13122496 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dong, Bo Zhang, Gaoqiang Zhang, Xiaodong Chen, Xufei Zhang, Meiling Li, Linglin Lin, Weiming Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus |
title | Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus |
title_full | Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus |
title_fullStr | Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus |
title_full_unstemmed | Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus |
title_short | Development of an Indirect ELISA Based on Spike Protein to Detect Antibodies against Feline Coronavirus |
title_sort | development of an indirect elisa based on spike protein to detect antibodies against feline coronavirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707903/ https://www.ncbi.nlm.nih.gov/pubmed/34960764 http://dx.doi.org/10.3390/v13122496 |
work_keys_str_mv | AT dongbo developmentofanindirectelisabasedonspikeproteintodetectantibodiesagainstfelinecoronavirus AT zhanggaoqiang developmentofanindirectelisabasedonspikeproteintodetectantibodiesagainstfelinecoronavirus AT zhangxiaodong developmentofanindirectelisabasedonspikeproteintodetectantibodiesagainstfelinecoronavirus AT chenxufei developmentofanindirectelisabasedonspikeproteintodetectantibodiesagainstfelinecoronavirus AT zhangmeiling developmentofanindirectelisabasedonspikeproteintodetectantibodiesagainstfelinecoronavirus AT lilinglin developmentofanindirectelisabasedonspikeproteintodetectantibodiesagainstfelinecoronavirus AT linweiming developmentofanindirectelisabasedonspikeproteintodetectantibodiesagainstfelinecoronavirus |