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MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice

MFN1 (Mitofusin 1) and MFN2 (Mitofusin 2) are GTPases essential for mitochondrial fusion. Published studies revealed crucial roles of both Mitofusins during embryonic development. Despite the unique mitochondrial organization in sperm flagella, the biological requirement in sperm development and fun...

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Detalles Bibliográficos
Autores principales: Miao, Junru, Chen, Wei, Wang, Pengxiang, Zhang, Xin, Wang, Lei, Wang, Shuai, Wang, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707932/
https://www.ncbi.nlm.nih.gov/pubmed/34948301
http://dx.doi.org/10.3390/ijms222413507
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author Miao, Junru
Chen, Wei
Wang, Pengxiang
Zhang, Xin
Wang, Lei
Wang, Shuai
Wang, Yuan
author_facet Miao, Junru
Chen, Wei
Wang, Pengxiang
Zhang, Xin
Wang, Lei
Wang, Shuai
Wang, Yuan
author_sort Miao, Junru
collection PubMed
description MFN1 (Mitofusin 1) and MFN2 (Mitofusin 2) are GTPases essential for mitochondrial fusion. Published studies revealed crucial roles of both Mitofusins during embryonic development. Despite the unique mitochondrial organization in sperm flagella, the biological requirement in sperm development and functions remain undefined. Here, using sperm-specific Cre drivers, we show that either Mfn1 or Mfn2 knockout in haploid germ cells does not affect male fertility. The Mfn1 and Mfn2 double knockout mice were further analyzed. We found no differences in testis morphology and weight between Mfn-deficient mice and their wild-type littermate controls. Spermatogenesis was normal in Mfn double knockout mice, in which properly developed TRA98+ germ cells, SYCP3+ spermatocytes, and TNP1+ spermatids/spermatozoa were detected in seminiferous tubules, indicating that sperm formation was not disrupted upon MFN deficiency. Collectively, our findings reveal that both MFN1 and MFN2 are dispensable for sperm development and functions in mice.
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spelling pubmed-87079322021-12-25 MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice Miao, Junru Chen, Wei Wang, Pengxiang Zhang, Xin Wang, Lei Wang, Shuai Wang, Yuan Int J Mol Sci Article MFN1 (Mitofusin 1) and MFN2 (Mitofusin 2) are GTPases essential for mitochondrial fusion. Published studies revealed crucial roles of both Mitofusins during embryonic development. Despite the unique mitochondrial organization in sperm flagella, the biological requirement in sperm development and functions remain undefined. Here, using sperm-specific Cre drivers, we show that either Mfn1 or Mfn2 knockout in haploid germ cells does not affect male fertility. The Mfn1 and Mfn2 double knockout mice were further analyzed. We found no differences in testis morphology and weight between Mfn-deficient mice and their wild-type littermate controls. Spermatogenesis was normal in Mfn double knockout mice, in which properly developed TRA98+ germ cells, SYCP3+ spermatocytes, and TNP1+ spermatids/spermatozoa were detected in seminiferous tubules, indicating that sperm formation was not disrupted upon MFN deficiency. Collectively, our findings reveal that both MFN1 and MFN2 are dispensable for sperm development and functions in mice. MDPI 2021-12-16 /pmc/articles/PMC8707932/ /pubmed/34948301 http://dx.doi.org/10.3390/ijms222413507 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Miao, Junru
Chen, Wei
Wang, Pengxiang
Zhang, Xin
Wang, Lei
Wang, Shuai
Wang, Yuan
MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice
title MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice
title_full MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice
title_fullStr MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice
title_full_unstemmed MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice
title_short MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice
title_sort mfn1 and mfn2 are dispensable for sperm development and functions in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707932/
https://www.ncbi.nlm.nih.gov/pubmed/34948301
http://dx.doi.org/10.3390/ijms222413507
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