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MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice
MFN1 (Mitofusin 1) and MFN2 (Mitofusin 2) are GTPases essential for mitochondrial fusion. Published studies revealed crucial roles of both Mitofusins during embryonic development. Despite the unique mitochondrial organization in sperm flagella, the biological requirement in sperm development and fun...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707932/ https://www.ncbi.nlm.nih.gov/pubmed/34948301 http://dx.doi.org/10.3390/ijms222413507 |
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author | Miao, Junru Chen, Wei Wang, Pengxiang Zhang, Xin Wang, Lei Wang, Shuai Wang, Yuan |
author_facet | Miao, Junru Chen, Wei Wang, Pengxiang Zhang, Xin Wang, Lei Wang, Shuai Wang, Yuan |
author_sort | Miao, Junru |
collection | PubMed |
description | MFN1 (Mitofusin 1) and MFN2 (Mitofusin 2) are GTPases essential for mitochondrial fusion. Published studies revealed crucial roles of both Mitofusins during embryonic development. Despite the unique mitochondrial organization in sperm flagella, the biological requirement in sperm development and functions remain undefined. Here, using sperm-specific Cre drivers, we show that either Mfn1 or Mfn2 knockout in haploid germ cells does not affect male fertility. The Mfn1 and Mfn2 double knockout mice were further analyzed. We found no differences in testis morphology and weight between Mfn-deficient mice and their wild-type littermate controls. Spermatogenesis was normal in Mfn double knockout mice, in which properly developed TRA98+ germ cells, SYCP3+ spermatocytes, and TNP1+ spermatids/spermatozoa were detected in seminiferous tubules, indicating that sperm formation was not disrupted upon MFN deficiency. Collectively, our findings reveal that both MFN1 and MFN2 are dispensable for sperm development and functions in mice. |
format | Online Article Text |
id | pubmed-8707932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87079322021-12-25 MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice Miao, Junru Chen, Wei Wang, Pengxiang Zhang, Xin Wang, Lei Wang, Shuai Wang, Yuan Int J Mol Sci Article MFN1 (Mitofusin 1) and MFN2 (Mitofusin 2) are GTPases essential for mitochondrial fusion. Published studies revealed crucial roles of both Mitofusins during embryonic development. Despite the unique mitochondrial organization in sperm flagella, the biological requirement in sperm development and functions remain undefined. Here, using sperm-specific Cre drivers, we show that either Mfn1 or Mfn2 knockout in haploid germ cells does not affect male fertility. The Mfn1 and Mfn2 double knockout mice were further analyzed. We found no differences in testis morphology and weight between Mfn-deficient mice and their wild-type littermate controls. Spermatogenesis was normal in Mfn double knockout mice, in which properly developed TRA98+ germ cells, SYCP3+ spermatocytes, and TNP1+ spermatids/spermatozoa were detected in seminiferous tubules, indicating that sperm formation was not disrupted upon MFN deficiency. Collectively, our findings reveal that both MFN1 and MFN2 are dispensable for sperm development and functions in mice. MDPI 2021-12-16 /pmc/articles/PMC8707932/ /pubmed/34948301 http://dx.doi.org/10.3390/ijms222413507 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Miao, Junru Chen, Wei Wang, Pengxiang Zhang, Xin Wang, Lei Wang, Shuai Wang, Yuan MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice |
title | MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice |
title_full | MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice |
title_fullStr | MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice |
title_full_unstemmed | MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice |
title_short | MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice |
title_sort | mfn1 and mfn2 are dispensable for sperm development and functions in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8707932/ https://www.ncbi.nlm.nih.gov/pubmed/34948301 http://dx.doi.org/10.3390/ijms222413507 |
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