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The Role of Filippi’s Glands in the Silk Moths Cocoon Construction

Filippi’s glands (FGs), formerly also called Lyonet’s glands, are accessory secretory structures of the labial (silk) glands of lepidopteran caterpillars, which were implicated to play an important role in the maturation of the silk material and the construction of the cocoon. In our previous study,...

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Autores principales: Sehadova, Hana, Zavodska, Radka, Rouhova, Lenka, Zurovec, Michal, Sauman, Ivo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708004/
https://www.ncbi.nlm.nih.gov/pubmed/34948319
http://dx.doi.org/10.3390/ijms222413523
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author Sehadova, Hana
Zavodska, Radka
Rouhova, Lenka
Zurovec, Michal
Sauman, Ivo
author_facet Sehadova, Hana
Zavodska, Radka
Rouhova, Lenka
Zurovec, Michal
Sauman, Ivo
author_sort Sehadova, Hana
collection PubMed
description Filippi’s glands (FGs), formerly also called Lyonet’s glands, are accessory secretory structures of the labial (silk) glands of lepidopteran caterpillars, which were implicated to play an important role in the maturation of the silk material and the construction of the cocoon. In our previous study, we have identified several species of giant silk moths that completely lack the FGs. Interestingly, the absence of FGs in these species correlates with the construction of a loose cocoon architecture. We investigated the functions of FGs by their surgical extirpation in the last instar larvae of the silkworm, Bombyx mori. We found that the absence of FGs altered the structure of the resulting cocoon, in which the different layers of silk were separated. In further experiments, we found no effects of the absence of FGs on larval cocoon formation behavior or on changes in cocoon mass or lipid content. Differential proteomic analysis revealed no significant contribution of structural proteins from FGs to silk cocoon material, but we identified several low abundance proteins that may play a role in posttranslational modifications of some silk proteins. Proteomic analysis also revealed a difference in phosphorylation of the N-terminal sequence of fibroin-heavy chain molecule. Thus, FGs appear to affect silk stickiness during spinning by regulating posttranslational modifications. This could also explain the link that exists between the absence of these glands and the formation of loose cocoons in some giant silk moth species.
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spelling pubmed-87080042021-12-25 The Role of Filippi’s Glands in the Silk Moths Cocoon Construction Sehadova, Hana Zavodska, Radka Rouhova, Lenka Zurovec, Michal Sauman, Ivo Int J Mol Sci Article Filippi’s glands (FGs), formerly also called Lyonet’s glands, are accessory secretory structures of the labial (silk) glands of lepidopteran caterpillars, which were implicated to play an important role in the maturation of the silk material and the construction of the cocoon. In our previous study, we have identified several species of giant silk moths that completely lack the FGs. Interestingly, the absence of FGs in these species correlates with the construction of a loose cocoon architecture. We investigated the functions of FGs by their surgical extirpation in the last instar larvae of the silkworm, Bombyx mori. We found that the absence of FGs altered the structure of the resulting cocoon, in which the different layers of silk were separated. In further experiments, we found no effects of the absence of FGs on larval cocoon formation behavior or on changes in cocoon mass or lipid content. Differential proteomic analysis revealed no significant contribution of structural proteins from FGs to silk cocoon material, but we identified several low abundance proteins that may play a role in posttranslational modifications of some silk proteins. Proteomic analysis also revealed a difference in phosphorylation of the N-terminal sequence of fibroin-heavy chain molecule. Thus, FGs appear to affect silk stickiness during spinning by regulating posttranslational modifications. This could also explain the link that exists between the absence of these glands and the formation of loose cocoons in some giant silk moth species. MDPI 2021-12-16 /pmc/articles/PMC8708004/ /pubmed/34948319 http://dx.doi.org/10.3390/ijms222413523 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sehadova, Hana
Zavodska, Radka
Rouhova, Lenka
Zurovec, Michal
Sauman, Ivo
The Role of Filippi’s Glands in the Silk Moths Cocoon Construction
title The Role of Filippi’s Glands in the Silk Moths Cocoon Construction
title_full The Role of Filippi’s Glands in the Silk Moths Cocoon Construction
title_fullStr The Role of Filippi’s Glands in the Silk Moths Cocoon Construction
title_full_unstemmed The Role of Filippi’s Glands in the Silk Moths Cocoon Construction
title_short The Role of Filippi’s Glands in the Silk Moths Cocoon Construction
title_sort role of filippi’s glands in the silk moths cocoon construction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708004/
https://www.ncbi.nlm.nih.gov/pubmed/34948319
http://dx.doi.org/10.3390/ijms222413523
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