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New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm

Wheat blast (WB) disease, since its first identification in Bangladesh in 2016, is now an established serious threat to wheat production in South Asia. There is a need for sound knowledge about resistance sources and associated genomic regions to assist breeding programs. Hence, a panel of genotypes...

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Autores principales: Roy, Chandan, Juliana, Philomin, Kabir, Muhammad R., Roy, Krishna K., Gahtyari, Navin C., Marza, Felix, He, Xinyao, Singh, Gyanendra P., Chawade, Aakash, Joshi, Arun K., Singh, Pawan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708018/
https://www.ncbi.nlm.nih.gov/pubmed/34961165
http://dx.doi.org/10.3390/plants10122693
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author Roy, Chandan
Juliana, Philomin
Kabir, Muhammad R.
Roy, Krishna K.
Gahtyari, Navin C.
Marza, Felix
He, Xinyao
Singh, Gyanendra P.
Chawade, Aakash
Joshi, Arun K.
Singh, Pawan K.
author_facet Roy, Chandan
Juliana, Philomin
Kabir, Muhammad R.
Roy, Krishna K.
Gahtyari, Navin C.
Marza, Felix
He, Xinyao
Singh, Gyanendra P.
Chawade, Aakash
Joshi, Arun K.
Singh, Pawan K.
author_sort Roy, Chandan
collection PubMed
description Wheat blast (WB) disease, since its first identification in Bangladesh in 2016, is now an established serious threat to wheat production in South Asia. There is a need for sound knowledge about resistance sources and associated genomic regions to assist breeding programs. Hence, a panel of genotypes from India and Bangladesh was evaluated for wheat blast resistance and a genome-wide association study (GWAS) was performed. Disease evaluation was done during five crop seasons—at precision phenotyping platform (PPPs) for wheat blast disease at Jashore (2018–19), Quirusillas (2018–19 and 2019–20) and Okinawa (2019 and 2020). Single nucleotide polymorphisms (SNP) across the genome were obtained using DArTseq genotyping-by-sequencing platform, and in total 5713 filtered markers were used. GWAS revealed 40 significant markers associated with WB resistance, of which 33 (82.5%) were in the 2NS/2AS chromosome segment and one each on seven chromosomes (3B, 3D, 4A, 5A, 5D, 6A and 6B). The 2NS markers contributed significantly in most of the environments, explaining an average of 33.4% of the phenotypic variation. Overall, 22.4% of the germplasm carried 2NS/2AS segment. So far, 2NS translocation is the only effective WB resistance source being used in the breeding programs of South Asia. Nevertheless, the identification of non-2NS/2AS genomic regions for WB resistance provides a hope to broaden and diversify resistance for this disease in years to come.
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spelling pubmed-87080182021-12-25 New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm Roy, Chandan Juliana, Philomin Kabir, Muhammad R. Roy, Krishna K. Gahtyari, Navin C. Marza, Felix He, Xinyao Singh, Gyanendra P. Chawade, Aakash Joshi, Arun K. Singh, Pawan K. Plants (Basel) Article Wheat blast (WB) disease, since its first identification in Bangladesh in 2016, is now an established serious threat to wheat production in South Asia. There is a need for sound knowledge about resistance sources and associated genomic regions to assist breeding programs. Hence, a panel of genotypes from India and Bangladesh was evaluated for wheat blast resistance and a genome-wide association study (GWAS) was performed. Disease evaluation was done during five crop seasons—at precision phenotyping platform (PPPs) for wheat blast disease at Jashore (2018–19), Quirusillas (2018–19 and 2019–20) and Okinawa (2019 and 2020). Single nucleotide polymorphisms (SNP) across the genome were obtained using DArTseq genotyping-by-sequencing platform, and in total 5713 filtered markers were used. GWAS revealed 40 significant markers associated with WB resistance, of which 33 (82.5%) were in the 2NS/2AS chromosome segment and one each on seven chromosomes (3B, 3D, 4A, 5A, 5D, 6A and 6B). The 2NS markers contributed significantly in most of the environments, explaining an average of 33.4% of the phenotypic variation. Overall, 22.4% of the germplasm carried 2NS/2AS segment. So far, 2NS translocation is the only effective WB resistance source being used in the breeding programs of South Asia. Nevertheless, the identification of non-2NS/2AS genomic regions for WB resistance provides a hope to broaden and diversify resistance for this disease in years to come. MDPI 2021-12-08 /pmc/articles/PMC8708018/ /pubmed/34961165 http://dx.doi.org/10.3390/plants10122693 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Roy, Chandan
Juliana, Philomin
Kabir, Muhammad R.
Roy, Krishna K.
Gahtyari, Navin C.
Marza, Felix
He, Xinyao
Singh, Gyanendra P.
Chawade, Aakash
Joshi, Arun K.
Singh, Pawan K.
New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm
title New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm
title_full New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm
title_fullStr New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm
title_full_unstemmed New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm
title_short New Genotypes and Genomic Regions for Resistance to Wheat Blast in South Asian Germplasm
title_sort new genotypes and genomic regions for resistance to wheat blast in south asian germplasm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708018/
https://www.ncbi.nlm.nih.gov/pubmed/34961165
http://dx.doi.org/10.3390/plants10122693
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