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Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi

Belowground interactions of plants with other organisms in the rhizosphere rely on extensive small-molecule communication. Chemical signals released from host plant roots ensure the development of beneficial arbuscular mycorrhizal (AM) fungi which in turn modulate host plant growth and stress tolera...

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Autores principales: Mishev, Kiril, Dobrev, Petre I., Lacek, Jozef, Filepová, Roberta, Yuperlieva-Mateeva, Bistra, Kostadinova, Anelia, Hristeva, Tsveta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708155/
https://www.ncbi.nlm.nih.gov/pubmed/34948474
http://dx.doi.org/10.3390/ijms222413677
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author Mishev, Kiril
Dobrev, Petre I.
Lacek, Jozef
Filepová, Roberta
Yuperlieva-Mateeva, Bistra
Kostadinova, Anelia
Hristeva, Tsveta
author_facet Mishev, Kiril
Dobrev, Petre I.
Lacek, Jozef
Filepová, Roberta
Yuperlieva-Mateeva, Bistra
Kostadinova, Anelia
Hristeva, Tsveta
author_sort Mishev, Kiril
collection PubMed
description Belowground interactions of plants with other organisms in the rhizosphere rely on extensive small-molecule communication. Chemical signals released from host plant roots ensure the development of beneficial arbuscular mycorrhizal (AM) fungi which in turn modulate host plant growth and stress tolerance. However, parasitic plants have adopted the capacity to sense the same signaling molecules and to trigger their own seed germination in the immediate vicinity of host roots. The contribution of AM fungi and parasitic plants to the regulation of phytohormone levels in host plant roots and root exudates remains largely obscure. Here, we studied the hormonome in the model system comprising tobacco as a host plant, Phelipanche spp. as a holoparasitic plant, and the AM fungus Rhizophagus irregularis. Co-cultivation of tobacco with broomrape and AM fungi alone or in combination led to characteristic changes in the levels of endogenous and exuded abscisic acid, indole-3-acetic acid, cytokinins, salicylic acid, and orobanchol-type strigolactones. The hormonal content in exudates of broomrape-infested mycorrhizal roots resembled that in exudates of infested non-mycorrhizal roots and differed from that observed in exudates of non-infested mycorrhizal roots. Moreover, we observed a significant reduction in AM colonization of infested tobacco plants, pointing to a dominant role of the holoparasite within the tripartite system.
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spelling pubmed-87081552021-12-25 Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi Mishev, Kiril Dobrev, Petre I. Lacek, Jozef Filepová, Roberta Yuperlieva-Mateeva, Bistra Kostadinova, Anelia Hristeva, Tsveta Int J Mol Sci Article Belowground interactions of plants with other organisms in the rhizosphere rely on extensive small-molecule communication. Chemical signals released from host plant roots ensure the development of beneficial arbuscular mycorrhizal (AM) fungi which in turn modulate host plant growth and stress tolerance. However, parasitic plants have adopted the capacity to sense the same signaling molecules and to trigger their own seed germination in the immediate vicinity of host roots. The contribution of AM fungi and parasitic plants to the regulation of phytohormone levels in host plant roots and root exudates remains largely obscure. Here, we studied the hormonome in the model system comprising tobacco as a host plant, Phelipanche spp. as a holoparasitic plant, and the AM fungus Rhizophagus irregularis. Co-cultivation of tobacco with broomrape and AM fungi alone or in combination led to characteristic changes in the levels of endogenous and exuded abscisic acid, indole-3-acetic acid, cytokinins, salicylic acid, and orobanchol-type strigolactones. The hormonal content in exudates of broomrape-infested mycorrhizal roots resembled that in exudates of infested non-mycorrhizal roots and differed from that observed in exudates of non-infested mycorrhizal roots. Moreover, we observed a significant reduction in AM colonization of infested tobacco plants, pointing to a dominant role of the holoparasite within the tripartite system. MDPI 2021-12-20 /pmc/articles/PMC8708155/ /pubmed/34948474 http://dx.doi.org/10.3390/ijms222413677 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mishev, Kiril
Dobrev, Petre I.
Lacek, Jozef
Filepová, Roberta
Yuperlieva-Mateeva, Bistra
Kostadinova, Anelia
Hristeva, Tsveta
Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi
title Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi
title_full Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi
title_fullStr Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi
title_full_unstemmed Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi
title_short Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi
title_sort hormonomic changes driving the negative impact of broomrape on plant host interactions with arbuscular mycorrhizal fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708155/
https://www.ncbi.nlm.nih.gov/pubmed/34948474
http://dx.doi.org/10.3390/ijms222413677
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