Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro

High-throughput screening of the NatureBank marine extract library (n = 7616) using a phenotypic assay for the parasitic nematode Haemonchus contortus identified an active extract derived from the Australian marine sponge Citronia sp. Bioassay-guided fractionation of the CH(2)Cl(2)/MeOH extract from...

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Autores principales: Ramage, Kelsey S., Taki, Aya C., Lum, Kah Yean, Hayes, Sasha, Byrne, Joseph J., Wang, Tao, Hofmann, Andreas, Ekins, Merrick G., White, Jonathan M., Jabbar, Abdul, Davis, Rohan A., Gasser, Robin B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708643/
https://www.ncbi.nlm.nih.gov/pubmed/34940697
http://dx.doi.org/10.3390/md19120698
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author Ramage, Kelsey S.
Taki, Aya C.
Lum, Kah Yean
Hayes, Sasha
Byrne, Joseph J.
Wang, Tao
Hofmann, Andreas
Ekins, Merrick G.
White, Jonathan M.
Jabbar, Abdul
Davis, Rohan A.
Gasser, Robin B.
author_facet Ramage, Kelsey S.
Taki, Aya C.
Lum, Kah Yean
Hayes, Sasha
Byrne, Joseph J.
Wang, Tao
Hofmann, Andreas
Ekins, Merrick G.
White, Jonathan M.
Jabbar, Abdul
Davis, Rohan A.
Gasser, Robin B.
author_sort Ramage, Kelsey S.
collection PubMed
description High-throughput screening of the NatureBank marine extract library (n = 7616) using a phenotypic assay for the parasitic nematode Haemonchus contortus identified an active extract derived from the Australian marine sponge Citronia sp. Bioassay-guided fractionation of the CH(2)Cl(2)/MeOH extract from Citronia sp. resulted in the purification of two known hexachlorinated peptides, dysidenin (1) and dysideathiazole (2). Compound 1 inhibited the growth/development of H. contortus larvae and induced multiple phenotypic changes, including a lethal evisceration (Evi) phenotype and/or somatic cell and tissue destruction. This is the first report of anthelmintic activity for these rare and unique polychlorinated peptides.
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spelling pubmed-87086432021-12-25 Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro Ramage, Kelsey S. Taki, Aya C. Lum, Kah Yean Hayes, Sasha Byrne, Joseph J. Wang, Tao Hofmann, Andreas Ekins, Merrick G. White, Jonathan M. Jabbar, Abdul Davis, Rohan A. Gasser, Robin B. Mar Drugs Article High-throughput screening of the NatureBank marine extract library (n = 7616) using a phenotypic assay for the parasitic nematode Haemonchus contortus identified an active extract derived from the Australian marine sponge Citronia sp. Bioassay-guided fractionation of the CH(2)Cl(2)/MeOH extract from Citronia sp. resulted in the purification of two known hexachlorinated peptides, dysidenin (1) and dysideathiazole (2). Compound 1 inhibited the growth/development of H. contortus larvae and induced multiple phenotypic changes, including a lethal evisceration (Evi) phenotype and/or somatic cell and tissue destruction. This is the first report of anthelmintic activity for these rare and unique polychlorinated peptides. MDPI 2021-12-09 /pmc/articles/PMC8708643/ /pubmed/34940697 http://dx.doi.org/10.3390/md19120698 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ramage, Kelsey S.
Taki, Aya C.
Lum, Kah Yean
Hayes, Sasha
Byrne, Joseph J.
Wang, Tao
Hofmann, Andreas
Ekins, Merrick G.
White, Jonathan M.
Jabbar, Abdul
Davis, Rohan A.
Gasser, Robin B.
Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro
title Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro
title_full Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro
title_fullStr Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro
title_full_unstemmed Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro
title_short Dysidenin from the Marine Sponge Citronia sp. Affects the Motility and Morphology of Haemonchus contortus Larvae In Vitro
title_sort dysidenin from the marine sponge citronia sp. affects the motility and morphology of haemonchus contortus larvae in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708643/
https://www.ncbi.nlm.nih.gov/pubmed/34940697
http://dx.doi.org/10.3390/md19120698
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