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Purification of Porcine Circovirus Type 2 Using an Affinity Chromatography Based on a Neutralizing Monoclonal Antibody against Viral Capsid Protein
Porcine circovirus type 2 (PCV2) is a DNA virus without an envelope. The viral particle is icosahedral and has a diameter of approximately 17 nm. In order to obtain the purified virus, a broad-spectrum monoclonal antibody 3A5 against PCV2 was coupled to CNBr-activated Sepharose(TM) 4B, and an affini...
Autores principales: | Bian, Haiqiao, Yu, Chong, Wei, Yanwu, Feng, Li, Liu, Changming, Huang, Liping |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708674/ https://www.ncbi.nlm.nih.gov/pubmed/34959519 http://dx.doi.org/10.3390/pathogens10121564 |
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