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3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine

With the aim of fabricating drug-loaded implantable patches, a 3D printing technique was employed to produce novel coaxial hydrogel patches. The core-section of these patches contained a dopamine-modified methacrylated alginate hydrogel loaded with a chemotherapeutic drug (Gemcitabine), while their...

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Autores principales: Talebian, Sepehr, Shim, In Kyong, Foroughi, Javad, Orive, Gorka, Vine, Kara L., Kim, Song Cheol, Wallace, Gordon G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708853/
https://www.ncbi.nlm.nih.gov/pubmed/34960917
http://dx.doi.org/10.3390/polym13244367
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author Talebian, Sepehr
Shim, In Kyong
Foroughi, Javad
Orive, Gorka
Vine, Kara L.
Kim, Song Cheol
Wallace, Gordon G.
author_facet Talebian, Sepehr
Shim, In Kyong
Foroughi, Javad
Orive, Gorka
Vine, Kara L.
Kim, Song Cheol
Wallace, Gordon G.
author_sort Talebian, Sepehr
collection PubMed
description With the aim of fabricating drug-loaded implantable patches, a 3D printing technique was employed to produce novel coaxial hydrogel patches. The core-section of these patches contained a dopamine-modified methacrylated alginate hydrogel loaded with a chemotherapeutic drug (Gemcitabine), while their shell section was solely comprised of a methacrylated alginate hydrogel. Subsequently, these patches were further modified with CaCO(3) cross linker and a polylactic acid (PLA) coating to facilitate prolonged release of the drug. Consequently, the results showed that addition of CaCO(3) to the formula enhanced the mechanical properties of the patches and significantly reduced their swelling ratio as compared to that for patches without CaCO(3). Furthermore, addition of PLA coating to CaCO(3)-containing patches has further reduced their swelling ratio, which then significantly slowed down the release of Gemcitabine, to a point where 4-layered patches could release the drug over a period of 7 days in vitro. Remarkably, it was shown that 3-layered and 4-layered Gemcitabine loaded patches were successful in inhibiting pancreatic cancer cell growth for a period of 14 days when tested in vitro. Lastly, in vivo experiments showed that gemcitabine-loaded 4-layered patches were capable of reducing the tumor growth rate and caused no severe toxicity when tested in mice. Altogether, 3D printed hydrogel patches might be used as biocompatible implants for local delivery of drugs to diseased site, to either shrink the tumor or to prevent the tumor recurrence after resection.
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spelling pubmed-87088532021-12-25 3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine Talebian, Sepehr Shim, In Kyong Foroughi, Javad Orive, Gorka Vine, Kara L. Kim, Song Cheol Wallace, Gordon G. Polymers (Basel) Article With the aim of fabricating drug-loaded implantable patches, a 3D printing technique was employed to produce novel coaxial hydrogel patches. The core-section of these patches contained a dopamine-modified methacrylated alginate hydrogel loaded with a chemotherapeutic drug (Gemcitabine), while their shell section was solely comprised of a methacrylated alginate hydrogel. Subsequently, these patches were further modified with CaCO(3) cross linker and a polylactic acid (PLA) coating to facilitate prolonged release of the drug. Consequently, the results showed that addition of CaCO(3) to the formula enhanced the mechanical properties of the patches and significantly reduced their swelling ratio as compared to that for patches without CaCO(3). Furthermore, addition of PLA coating to CaCO(3)-containing patches has further reduced their swelling ratio, which then significantly slowed down the release of Gemcitabine, to a point where 4-layered patches could release the drug over a period of 7 days in vitro. Remarkably, it was shown that 3-layered and 4-layered Gemcitabine loaded patches were successful in inhibiting pancreatic cancer cell growth for a period of 14 days when tested in vitro. Lastly, in vivo experiments showed that gemcitabine-loaded 4-layered patches were capable of reducing the tumor growth rate and caused no severe toxicity when tested in mice. Altogether, 3D printed hydrogel patches might be used as biocompatible implants for local delivery of drugs to diseased site, to either shrink the tumor or to prevent the tumor recurrence after resection. MDPI 2021-12-13 /pmc/articles/PMC8708853/ /pubmed/34960917 http://dx.doi.org/10.3390/polym13244367 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Talebian, Sepehr
Shim, In Kyong
Foroughi, Javad
Orive, Gorka
Vine, Kara L.
Kim, Song Cheol
Wallace, Gordon G.
3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine
title 3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine
title_full 3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine
title_fullStr 3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine
title_full_unstemmed 3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine
title_short 3D-Printed Coaxial Hydrogel Patches with Mussel-Inspired Elements for Prolonged Release of Gemcitabine
title_sort 3d-printed coaxial hydrogel patches with mussel-inspired elements for prolonged release of gemcitabine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708853/
https://www.ncbi.nlm.nih.gov/pubmed/34960917
http://dx.doi.org/10.3390/polym13244367
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