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Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis
The bacterial proteins of the Dsb family catalyze the formation of disulfide bridges between cysteine residues that stabilize protein structures and ensure their proper functioning. Here, we report the detailed analysis of the Dsb pathway of Campylobacter jejuni. The oxidizing Dsb system of this pat...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708908/ https://www.ncbi.nlm.nih.gov/pubmed/34948248 http://dx.doi.org/10.3390/ijms222413451 |
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author | Banaś, Anna M. Bocian-Ostrzycka, Katarzyna M. Dunin-Horkawicz, Stanisław Ludwiczak, Jan Wilk, Piotr Orlikowska, Marta Wyszyńska, Agnieszka Dąbrowska, Maria Plichta, Maciej Spodzieja, Marta Polańska, Marta A. Malinowska, Agata Jagusztyn-Krynicka, Elżbieta Katarzyna |
author_facet | Banaś, Anna M. Bocian-Ostrzycka, Katarzyna M. Dunin-Horkawicz, Stanisław Ludwiczak, Jan Wilk, Piotr Orlikowska, Marta Wyszyńska, Agnieszka Dąbrowska, Maria Plichta, Maciej Spodzieja, Marta Polańska, Marta A. Malinowska, Agata Jagusztyn-Krynicka, Elżbieta Katarzyna |
author_sort | Banaś, Anna M. |
collection | PubMed |
description | The bacterial proteins of the Dsb family catalyze the formation of disulfide bridges between cysteine residues that stabilize protein structures and ensure their proper functioning. Here, we report the detailed analysis of the Dsb pathway of Campylobacter jejuni. The oxidizing Dsb system of this pathogen is unique because it consists of two monomeric DsbAs (DsbA1 and DsbA2) and one dimeric bifunctional protein (C8J_1298). Previously, we showed that DsbA1 and C8J_1298 are redundant. Here, we unraveled the interaction between the two monomeric DsbAs by in vitro and in vivo experiments and by solving their structures and found that both monomeric DsbAs are dispensable proteins. Their structures confirmed that they are homologs of EcDsbL. The slight differences seen in the surface charge of the proteins do not affect the interaction with their redox partner. Comparative proteomics showed that several respiratory proteins, as well as periplasmic transport proteins, are targets of the Dsb system. Some of these, both donors and electron acceptors, are essential elements of the C. jejuni respiratory process under oxygen-limiting conditions in the host intestine. The data presented provide detailed information on the function of the C. jejuni Dsb system, identifying it as a potential target for novel antibacterial molecules. |
format | Online Article Text |
id | pubmed-8708908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87089082021-12-25 Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis Banaś, Anna M. Bocian-Ostrzycka, Katarzyna M. Dunin-Horkawicz, Stanisław Ludwiczak, Jan Wilk, Piotr Orlikowska, Marta Wyszyńska, Agnieszka Dąbrowska, Maria Plichta, Maciej Spodzieja, Marta Polańska, Marta A. Malinowska, Agata Jagusztyn-Krynicka, Elżbieta Katarzyna Int J Mol Sci Article The bacterial proteins of the Dsb family catalyze the formation of disulfide bridges between cysteine residues that stabilize protein structures and ensure their proper functioning. Here, we report the detailed analysis of the Dsb pathway of Campylobacter jejuni. The oxidizing Dsb system of this pathogen is unique because it consists of two monomeric DsbAs (DsbA1 and DsbA2) and one dimeric bifunctional protein (C8J_1298). Previously, we showed that DsbA1 and C8J_1298 are redundant. Here, we unraveled the interaction between the two monomeric DsbAs by in vitro and in vivo experiments and by solving their structures and found that both monomeric DsbAs are dispensable proteins. Their structures confirmed that they are homologs of EcDsbL. The slight differences seen in the surface charge of the proteins do not affect the interaction with their redox partner. Comparative proteomics showed that several respiratory proteins, as well as periplasmic transport proteins, are targets of the Dsb system. Some of these, both donors and electron acceptors, are essential elements of the C. jejuni respiratory process under oxygen-limiting conditions in the host intestine. The data presented provide detailed information on the function of the C. jejuni Dsb system, identifying it as a potential target for novel antibacterial molecules. MDPI 2021-12-15 /pmc/articles/PMC8708908/ /pubmed/34948248 http://dx.doi.org/10.3390/ijms222413451 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Banaś, Anna M. Bocian-Ostrzycka, Katarzyna M. Dunin-Horkawicz, Stanisław Ludwiczak, Jan Wilk, Piotr Orlikowska, Marta Wyszyńska, Agnieszka Dąbrowska, Maria Plichta, Maciej Spodzieja, Marta Polańska, Marta A. Malinowska, Agata Jagusztyn-Krynicka, Elżbieta Katarzyna Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis |
title | Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis |
title_full | Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis |
title_fullStr | Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis |
title_full_unstemmed | Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis |
title_short | Interplay between DsbA1, DsbA2 and C8J_1298 Periplasmic Oxidoreductases of Campylobacter jejuni and Their Impact on Bacterial Physiology and Pathogenesis |
title_sort | interplay between dsba1, dsba2 and c8j_1298 periplasmic oxidoreductases of campylobacter jejuni and their impact on bacterial physiology and pathogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8708908/ https://www.ncbi.nlm.nih.gov/pubmed/34948248 http://dx.doi.org/10.3390/ijms222413451 |
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