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Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential

Most plants rely on specialized root-associated microbes to obtain essential nitrogen (N), yet not much is known about the evolutionary history of the rhizosphere–plant interaction. We conducted a common garden experiment to investigate the plant root–rhizosphere microbiome association using chlorid...

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Autores principales: Van Bel, Mikayla, Fisher, Amanda E., Ball, Laymon, Columbus, J. Travis, Berlemont, Renaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709245/
https://www.ncbi.nlm.nih.gov/pubmed/34946078
http://dx.doi.org/10.3390/microorganisms9122476
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author Van Bel, Mikayla
Fisher, Amanda E.
Ball, Laymon
Columbus, J. Travis
Berlemont, Renaud
author_facet Van Bel, Mikayla
Fisher, Amanda E.
Ball, Laymon
Columbus, J. Travis
Berlemont, Renaud
author_sort Van Bel, Mikayla
collection PubMed
description Most plants rely on specialized root-associated microbes to obtain essential nitrogen (N), yet not much is known about the evolutionary history of the rhizosphere–plant interaction. We conducted a common garden experiment to investigate the plant root–rhizosphere microbiome association using chloridoid grasses sampled from around the world and grown from seed in a greenhouse. We sought to test whether plants that are more closely related phylogenetically have more similar root bacterial microbiomes than plants that are more distantly related. Using metagenome sequencing, we found that there is a conserved core and a variable rhizosphere bacterial microbiome across the chloridoid grasses. Additionally, phylogenetic distance among the host plant species was correlated with bacterial community composition, suggesting the plant hosts prefer specific bacterial lineages. The functional potential for N utilization across microbiomes fluctuated extensively and mirrored variation in the microbial community composition across host plants. Variation in the bacterial potential for N fixation was strongly affected by the host plants’ phylogeny, whereas variation in N recycling, nitrification, and denitrification was unaffected. This study highlights the evolutionary linkage between the N fixation traits of the microbial community and the plant host and suggests that not all functional traits are equally important for plant–microbe associations.
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spelling pubmed-87092452021-12-25 Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential Van Bel, Mikayla Fisher, Amanda E. Ball, Laymon Columbus, J. Travis Berlemont, Renaud Microorganisms Article Most plants rely on specialized root-associated microbes to obtain essential nitrogen (N), yet not much is known about the evolutionary history of the rhizosphere–plant interaction. We conducted a common garden experiment to investigate the plant root–rhizosphere microbiome association using chloridoid grasses sampled from around the world and grown from seed in a greenhouse. We sought to test whether plants that are more closely related phylogenetically have more similar root bacterial microbiomes than plants that are more distantly related. Using metagenome sequencing, we found that there is a conserved core and a variable rhizosphere bacterial microbiome across the chloridoid grasses. Additionally, phylogenetic distance among the host plant species was correlated with bacterial community composition, suggesting the plant hosts prefer specific bacterial lineages. The functional potential for N utilization across microbiomes fluctuated extensively and mirrored variation in the microbial community composition across host plants. Variation in the bacterial potential for N fixation was strongly affected by the host plants’ phylogeny, whereas variation in N recycling, nitrification, and denitrification was unaffected. This study highlights the evolutionary linkage between the N fixation traits of the microbial community and the plant host and suggests that not all functional traits are equally important for plant–microbe associations. MDPI 2021-11-30 /pmc/articles/PMC8709245/ /pubmed/34946078 http://dx.doi.org/10.3390/microorganisms9122476 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Van Bel, Mikayla
Fisher, Amanda E.
Ball, Laymon
Columbus, J. Travis
Berlemont, Renaud
Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential
title Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential
title_full Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential
title_fullStr Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential
title_full_unstemmed Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential
title_short Phylosymbiosis in the Rhizosphere Microbiome Extends to Nitrogen Cycle Functional Potential
title_sort phylosymbiosis in the rhizosphere microbiome extends to nitrogen cycle functional potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709245/
https://www.ncbi.nlm.nih.gov/pubmed/34946078
http://dx.doi.org/10.3390/microorganisms9122476
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