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Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro

In recent years, the study of dermal preparations has received increased attention. There are more and more modern approaches to evaluate transdermal formulations, which are crucial in proving the efficacy of a formulation. The aim of this study was to compare permeation across innovative synthetic...

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Autores principales: Kovács, Anita, Zsikó, Stella, Falusi, Fanni, Csányi, Erzsébet, Budai-Szűcs, Mária, Csóka, Ildikó, Berkó, Szilvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709252/
https://www.ncbi.nlm.nih.gov/pubmed/34959387
http://dx.doi.org/10.3390/pharmaceutics13122106
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author Kovács, Anita
Zsikó, Stella
Falusi, Fanni
Csányi, Erzsébet
Budai-Szűcs, Mária
Csóka, Ildikó
Berkó, Szilvia
author_facet Kovács, Anita
Zsikó, Stella
Falusi, Fanni
Csányi, Erzsébet
Budai-Szűcs, Mária
Csóka, Ildikó
Berkó, Szilvia
author_sort Kovács, Anita
collection PubMed
description In recent years, the study of dermal preparations has received increased attention. There are more and more modern approaches to evaluate transdermal formulations, which are crucial in proving the efficacy of a formulation. The aim of this study was to compare permeation across innovative synthetic membranes (Strat-M and Skin PAMPA membranes) and heat-separated human epidermis (HSE, gold standard membrane) using four different dermal formulations. The Strat-M and Skin PAMPA membranes were designed to mimic the stratum corneum layer of the human epidermis. There have also been some publications on their use in dermal formulation development, but further information is needed. Drug permeation was measured using formulations containing diclofenac sodium (two hydrogels and two creams). The HSE, Strat-M, and Skin PAMPA membranes proved to be significantly different, but based on the results, the Strat-M membrane showed the greatest similarity to HSE. The permeation data of the different formulations across different membranes showed good correlations with formulations similar to these four, which allows the prediction of permeation across HSE using these synthetic membranes. In addition, Strat-M and Skin PAMPA membranes have the potential to select and differentiate a dermal formulation containing diclofenac sodium as an early screening model.
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spelling pubmed-87092522021-12-25 Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro Kovács, Anita Zsikó, Stella Falusi, Fanni Csányi, Erzsébet Budai-Szűcs, Mária Csóka, Ildikó Berkó, Szilvia Pharmaceutics Article In recent years, the study of dermal preparations has received increased attention. There are more and more modern approaches to evaluate transdermal formulations, which are crucial in proving the efficacy of a formulation. The aim of this study was to compare permeation across innovative synthetic membranes (Strat-M and Skin PAMPA membranes) and heat-separated human epidermis (HSE, gold standard membrane) using four different dermal formulations. The Strat-M and Skin PAMPA membranes were designed to mimic the stratum corneum layer of the human epidermis. There have also been some publications on their use in dermal formulation development, but further information is needed. Drug permeation was measured using formulations containing diclofenac sodium (two hydrogels and two creams). The HSE, Strat-M, and Skin PAMPA membranes proved to be significantly different, but based on the results, the Strat-M membrane showed the greatest similarity to HSE. The permeation data of the different formulations across different membranes showed good correlations with formulations similar to these four, which allows the prediction of permeation across HSE using these synthetic membranes. In addition, Strat-M and Skin PAMPA membranes have the potential to select and differentiate a dermal formulation containing diclofenac sodium as an early screening model. MDPI 2021-12-07 /pmc/articles/PMC8709252/ /pubmed/34959387 http://dx.doi.org/10.3390/pharmaceutics13122106 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kovács, Anita
Zsikó, Stella
Falusi, Fanni
Csányi, Erzsébet
Budai-Szűcs, Mária
Csóka, Ildikó
Berkó, Szilvia
Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro
title Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro
title_full Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro
title_fullStr Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro
title_full_unstemmed Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro
title_short Comparison of Synthetic Membranes to Heat-Separated Human Epidermis in Skin Permeation Studies In Vitro
title_sort comparison of synthetic membranes to heat-separated human epidermis in skin permeation studies in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709252/
https://www.ncbi.nlm.nih.gov/pubmed/34959387
http://dx.doi.org/10.3390/pharmaceutics13122106
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