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Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor
Pyrrolizidine alkaloids (PAs) are produced by plants as secondary compounds that are the most widely distributed natural toxins. There have been many cases of human toxicity caused by consumption of toxic plant species, as herbal teas and grain or grain products contaminated with PA-containing seeds...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709324/ https://www.ncbi.nlm.nih.gov/pubmed/34945435 http://dx.doi.org/10.3390/mi12121585 |
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author | Senturk, Huseyin Eksin, Ece Zeybek, Ulvi Erdem, Arzum |
author_facet | Senturk, Huseyin Eksin, Ece Zeybek, Ulvi Erdem, Arzum |
author_sort | Senturk, Huseyin |
collection | PubMed |
description | Pyrrolizidine alkaloids (PAs) are produced by plants as secondary compounds that are the most widely distributed natural toxins. There have been many cases of human toxicity caused by consumption of toxic plant species, as herbal teas and grain or grain products contaminated with PA-containing seeds have been reported. Companies that produce dried spices and tea leaves should examine the PA level in their products. For the first time in the literature, a simple and inexpensive electrochemical assay based on a single-use sensor was introduced for quantitative determination of senecionine (SEN) in the most frequently contaminated food sources. SEN was immobilized on a pencil graphite electrode surface by the passive adsorption technique. Differential pulse voltammetry (DPV) was used to evaluate the oxidation signal of SEN, which was observed to be around +0.95 V. The oxidation signal was specific to the SEN in the sample, and the current value was proportional to its concentration. The selectivity of our assay was also tested in the presence of other similar PAs such as intermedine, lycopsamine, and heliotrine. The detection limit is calculated by developed assay and found to be 5.45 µg/mL, which is an acceptable concentration value of SEN occurring at toxic levels for consumers. As an application of the developed sensor in food products, the electrochemical detection of SEN was successfully performed in flour and herbal tea products. |
format | Online Article Text |
id | pubmed-8709324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87093242021-12-25 Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor Senturk, Huseyin Eksin, Ece Zeybek, Ulvi Erdem, Arzum Micromachines (Basel) Article Pyrrolizidine alkaloids (PAs) are produced by plants as secondary compounds that are the most widely distributed natural toxins. There have been many cases of human toxicity caused by consumption of toxic plant species, as herbal teas and grain or grain products contaminated with PA-containing seeds have been reported. Companies that produce dried spices and tea leaves should examine the PA level in their products. For the first time in the literature, a simple and inexpensive electrochemical assay based on a single-use sensor was introduced for quantitative determination of senecionine (SEN) in the most frequently contaminated food sources. SEN was immobilized on a pencil graphite electrode surface by the passive adsorption technique. Differential pulse voltammetry (DPV) was used to evaluate the oxidation signal of SEN, which was observed to be around +0.95 V. The oxidation signal was specific to the SEN in the sample, and the current value was proportional to its concentration. The selectivity of our assay was also tested in the presence of other similar PAs such as intermedine, lycopsamine, and heliotrine. The detection limit is calculated by developed assay and found to be 5.45 µg/mL, which is an acceptable concentration value of SEN occurring at toxic levels for consumers. As an application of the developed sensor in food products, the electrochemical detection of SEN was successfully performed in flour and herbal tea products. MDPI 2021-12-20 /pmc/articles/PMC8709324/ /pubmed/34945435 http://dx.doi.org/10.3390/mi12121585 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Senturk, Huseyin Eksin, Ece Zeybek, Ulvi Erdem, Arzum Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor |
title | Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor |
title_full | Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor |
title_fullStr | Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor |
title_full_unstemmed | Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor |
title_short | Detection of Senecionine in Dietary Sources by Single-Use Electrochemical Sensor |
title_sort | detection of senecionine in dietary sources by single-use electrochemical sensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709324/ https://www.ncbi.nlm.nih.gov/pubmed/34945435 http://dx.doi.org/10.3390/mi12121585 |
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