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Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway

BACKGROUND: Gestational diabetes mellitus (GDM) has significant short and long-term health consequences for both the mother and child. There is limited but suggestive evidence that inulin could improve glucose tolerance during pregnancy. This study assessed the effect of inulin on glucose homeostasi...

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Autores principales: Miao, Miao, Dai, Yongmei, Rui, Can, Fan, Yuru, Wang, Xinyan, Fan, Chong, Mu, Juan, Hou, Wenwen, Dong, Zhiyong, Li, Ping, Sun, Guiju, Zeng, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709963/
https://www.ncbi.nlm.nih.gov/pubmed/34952629
http://dx.doi.org/10.1186/s13098-021-00768-8
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author Miao, Miao
Dai, Yongmei
Rui, Can
Fan, Yuru
Wang, Xinyan
Fan, Chong
Mu, Juan
Hou, Wenwen
Dong, Zhiyong
Li, Ping
Sun, Guiju
Zeng, Xin
author_facet Miao, Miao
Dai, Yongmei
Rui, Can
Fan, Yuru
Wang, Xinyan
Fan, Chong
Mu, Juan
Hou, Wenwen
Dong, Zhiyong
Li, Ping
Sun, Guiju
Zeng, Xin
author_sort Miao, Miao
collection PubMed
description BACKGROUND: Gestational diabetes mellitus (GDM) has significant short and long-term health consequences for both the mother and child. There is limited but suggestive evidence that inulin could improve glucose tolerance during pregnancy. This study assessed the effect of inulin on glucose homeostasis and elucidated the molecular mechanisms underlying the inulin-induced antidiabetic effects during pregnancy. METHOD: Female C57BL/6 mice were randomized to receive either no treatment, high-dose inulin and low-dose inulin for 7 weeks with measurement of biochemical profiles. A real-time(2) (RT(2)) profiler polymerase chain reaction (PCR) array involved in glycolipid metabolism was measured. RESULTS: Inulin treatment facilitated glucose homeostasis in a dose-dependent manner by decreasing fasting blood glucose, advanced glycation end products and total cholesterol, and improving glucose tolerance. Suppressing resistin (RETN) expression was observed in the inulin treatment group and the expression was significantly correlated with fasting blood glucose levels. The ratios of p-IRS to IRS and p-Akt to Akt in liver tissue and the ratio of p-Akt to Akt in adipose tissue as well as the expression level of GLUT4 increased significantly after inulin treatment. CONCLUSIONS: Our findings indicated improvement of glucose and lipid metabolism by inulin was to activate glucose transport through the translocation of GLUT4 which was mediated by insulin signaling pathway repairment due to decreased expression of RETN and enhanced phosphorylation of IRS and Akt in GDM mice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13098-021-00768-8.
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spelling pubmed-87099632022-01-05 Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway Miao, Miao Dai, Yongmei Rui, Can Fan, Yuru Wang, Xinyan Fan, Chong Mu, Juan Hou, Wenwen Dong, Zhiyong Li, Ping Sun, Guiju Zeng, Xin Diabetol Metab Syndr Research BACKGROUND: Gestational diabetes mellitus (GDM) has significant short and long-term health consequences for both the mother and child. There is limited but suggestive evidence that inulin could improve glucose tolerance during pregnancy. This study assessed the effect of inulin on glucose homeostasis and elucidated the molecular mechanisms underlying the inulin-induced antidiabetic effects during pregnancy. METHOD: Female C57BL/6 mice were randomized to receive either no treatment, high-dose inulin and low-dose inulin for 7 weeks with measurement of biochemical profiles. A real-time(2) (RT(2)) profiler polymerase chain reaction (PCR) array involved in glycolipid metabolism was measured. RESULTS: Inulin treatment facilitated glucose homeostasis in a dose-dependent manner by decreasing fasting blood glucose, advanced glycation end products and total cholesterol, and improving glucose tolerance. Suppressing resistin (RETN) expression was observed in the inulin treatment group and the expression was significantly correlated with fasting blood glucose levels. The ratios of p-IRS to IRS and p-Akt to Akt in liver tissue and the ratio of p-Akt to Akt in adipose tissue as well as the expression level of GLUT4 increased significantly after inulin treatment. CONCLUSIONS: Our findings indicated improvement of glucose and lipid metabolism by inulin was to activate glucose transport through the translocation of GLUT4 which was mediated by insulin signaling pathway repairment due to decreased expression of RETN and enhanced phosphorylation of IRS and Akt in GDM mice. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13098-021-00768-8. BioMed Central 2021-12-24 /pmc/articles/PMC8709963/ /pubmed/34952629 http://dx.doi.org/10.1186/s13098-021-00768-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Miao, Miao
Dai, Yongmei
Rui, Can
Fan, Yuru
Wang, Xinyan
Fan, Chong
Mu, Juan
Hou, Wenwen
Dong, Zhiyong
Li, Ping
Sun, Guiju
Zeng, Xin
Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway
title Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway
title_full Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway
title_fullStr Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway
title_full_unstemmed Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway
title_short Dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via RENT/AKT/IRS/GLUT4 pathway
title_sort dietary supplementation of inulin alleviates metabolism disorders in gestational diabetes mellitus mice via rent/akt/irs/glut4 pathway
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8709963/
https://www.ncbi.nlm.nih.gov/pubmed/34952629
http://dx.doi.org/10.1186/s13098-021-00768-8
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