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Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis

OBJECTIVE: To investigate the correlation of the Val109Asp polymorphism of the omentin-1 gene with the risk and severity of knee osteoarthritis (KOA) in a Chinese Han population. METHODS: This study enrolled 383 patients with primary KOA and 460 healthy controls. The genotypes were determined by the...

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Autores principales: Chen, Ruofei, Zhang, Yaqin, Xu, Honggang, Hu, Huaqing, Chen, Mingwei, Shuai, Zongwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710085/
https://www.ncbi.nlm.nih.gov/pubmed/34992344
http://dx.doi.org/10.2147/DDDT.S340410
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author Chen, Ruofei
Zhang, Yaqin
Xu, Honggang
Hu, Huaqing
Chen, Mingwei
Shuai, Zongwen
author_facet Chen, Ruofei
Zhang, Yaqin
Xu, Honggang
Hu, Huaqing
Chen, Mingwei
Shuai, Zongwen
author_sort Chen, Ruofei
collection PubMed
description OBJECTIVE: To investigate the correlation of the Val109Asp polymorphism of the omentin-1 gene with the risk and severity of knee osteoarthritis (KOA) in a Chinese Han population. METHODS: This study enrolled 383 patients with primary KOA and 460 healthy controls. The genotypes were determined by the detection of single nucleotide polymorphism. To explore the interaction between omentin-1 gene polymorphism and obesity and age, the body mass index (BMI) of 25 kg/m(2) and the age of 55 years old were preset as the cut-off value of stratified analysis. Furthermore, enzyme-linked immunosorbent assay was used to determine the levels of omentin-1, interleukin (IL)-1β, IL-6 in peripheral blood and synovial fluid and the contents of IL-1β, IL-6, metalloproteinase (MMP)-13 and collagen (COL)-II in the supernatant of knee joint cartilage tissue. RESULTS: The Val109Asp polymorphism of the omentin-1 gene showed no obvious correlation with KOA. Compared with Asp/Asp genotype carriers with BMI <25 kg/m(2) and age <55 years old, Val109 allele carriers with BMI≥25 kg/m(2) and age ≥55 years old had obviously increased risk of KOA (adjusted OR = 1.416, p = 0.042; adjusted OR = 1.735, p = 0.038, respectively). In the KOA group, only the omentin-1 levels were significantly lower in the plasma and synovial fluid of Ala/Ala genotype carriers than in those of Asp/Asp genotype carriers. Meanwhile, the proportion of patients with moderate–severe K-L Classification, the levels of IL-1β, IL-6 in synovial fluid and the expression levels of IL-1β, IL-6 and MMP-13 in cartilage tissue significantly increased (p < 0.05). By contrast, the expression level of COL-II in cartilage tissue significantly decreased (p < 0.05). CONCLUSIONS: The Val109Asp polymorphism of the omentin-1 gene may not be the primary pathogenic factor of KOA in Chinese. The Val/Val genotype can be regarded as a potential biomarker for the risk of KOA progression.
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spelling pubmed-87100852022-01-05 Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis Chen, Ruofei Zhang, Yaqin Xu, Honggang Hu, Huaqing Chen, Mingwei Shuai, Zongwen Drug Des Devel Ther Original Research OBJECTIVE: To investigate the correlation of the Val109Asp polymorphism of the omentin-1 gene with the risk and severity of knee osteoarthritis (KOA) in a Chinese Han population. METHODS: This study enrolled 383 patients with primary KOA and 460 healthy controls. The genotypes were determined by the detection of single nucleotide polymorphism. To explore the interaction between omentin-1 gene polymorphism and obesity and age, the body mass index (BMI) of 25 kg/m(2) and the age of 55 years old were preset as the cut-off value of stratified analysis. Furthermore, enzyme-linked immunosorbent assay was used to determine the levels of omentin-1, interleukin (IL)-1β, IL-6 in peripheral blood and synovial fluid and the contents of IL-1β, IL-6, metalloproteinase (MMP)-13 and collagen (COL)-II in the supernatant of knee joint cartilage tissue. RESULTS: The Val109Asp polymorphism of the omentin-1 gene showed no obvious correlation with KOA. Compared with Asp/Asp genotype carriers with BMI <25 kg/m(2) and age <55 years old, Val109 allele carriers with BMI≥25 kg/m(2) and age ≥55 years old had obviously increased risk of KOA (adjusted OR = 1.416, p = 0.042; adjusted OR = 1.735, p = 0.038, respectively). In the KOA group, only the omentin-1 levels were significantly lower in the plasma and synovial fluid of Ala/Ala genotype carriers than in those of Asp/Asp genotype carriers. Meanwhile, the proportion of patients with moderate–severe K-L Classification, the levels of IL-1β, IL-6 in synovial fluid and the expression levels of IL-1β, IL-6 and MMP-13 in cartilage tissue significantly increased (p < 0.05). By contrast, the expression level of COL-II in cartilage tissue significantly decreased (p < 0.05). CONCLUSIONS: The Val109Asp polymorphism of the omentin-1 gene may not be the primary pathogenic factor of KOA in Chinese. The Val/Val genotype can be regarded as a potential biomarker for the risk of KOA progression. Dove 2021-12-21 /pmc/articles/PMC8710085/ /pubmed/34992344 http://dx.doi.org/10.2147/DDDT.S340410 Text en © 2021 Chen et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Chen, Ruofei
Zhang, Yaqin
Xu, Honggang
Hu, Huaqing
Chen, Mingwei
Shuai, Zongwen
Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis
title Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis
title_full Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis
title_fullStr Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis
title_full_unstemmed Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis
title_short Val109Asp Polymorphism of the Omentin-1 Gene and Incidence of Knee Osteoarthritis in a Chinese Han Population: A Correlation Analysis
title_sort val109asp polymorphism of the omentin-1 gene and incidence of knee osteoarthritis in a chinese han population: a correlation analysis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710085/
https://www.ncbi.nlm.nih.gov/pubmed/34992344
http://dx.doi.org/10.2147/DDDT.S340410
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