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Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19

To detect and prevent emerging epidemics, discovery platforms are urgently needed, for the rapid development of diagnostic assays. Molecular diagnostic tests for COVID-19 were developed shortly after the isolation of SARS-CoV-2. However, serological tests based on antiviral antibody detection, revea...

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Autores principales: Varotto-Boccazzi, Ilaria, Manenti, Alessandro, Dapporto, Francesca, Gourlay, Louise J., Bisaglia, Beatrice, Gabrieli, Paolo, Forneris, Federico, Faravelli, Silvia, Bollati, Valentina, Rubolini, Diego, Zuccotti, Gianvincenzo, Montomoli, Emanuele, Epis, Sara, Bandi, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710741/
https://www.ncbi.nlm.nih.gov/pubmed/34966362
http://dx.doi.org/10.3389/fmicb.2021.736530
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author Varotto-Boccazzi, Ilaria
Manenti, Alessandro
Dapporto, Francesca
Gourlay, Louise J.
Bisaglia, Beatrice
Gabrieli, Paolo
Forneris, Federico
Faravelli, Silvia
Bollati, Valentina
Rubolini, Diego
Zuccotti, Gianvincenzo
Montomoli, Emanuele
Epis, Sara
Bandi, Claudio
author_facet Varotto-Boccazzi, Ilaria
Manenti, Alessandro
Dapporto, Francesca
Gourlay, Louise J.
Bisaglia, Beatrice
Gabrieli, Paolo
Forneris, Federico
Faravelli, Silvia
Bollati, Valentina
Rubolini, Diego
Zuccotti, Gianvincenzo
Montomoli, Emanuele
Epis, Sara
Bandi, Claudio
author_sort Varotto-Boccazzi, Ilaria
collection PubMed
description To detect and prevent emerging epidemics, discovery platforms are urgently needed, for the rapid development of diagnostic assays. Molecular diagnostic tests for COVID-19 were developed shortly after the isolation of SARS-CoV-2. However, serological tests based on antiviral antibody detection, revealing previous exposure to the virus, required longer testing phases, due to the need to obtain correctly folded and glycosylated antigens. The delay between the identification of a new virus and the development of reliable serodiagnostic tools limits our readiness to tackle future epidemics. We suggest that the protozoan Leishmania tarentolae can be used as an easy-to-handle microfactory for the rapid production of viral antigens to face emerging epidemics. We engineered L. tarentolae to express the SARS-CoV-2 receptor-binding domain (RBD) and we recorded the ability of the purified RBD antigen to detect SARS-CoV-2 infection in human sera, with a sensitivity and reproducibility comparable to that of a reference antigen produced in human cells. This is the first application of an antigen produced in L. tarentolae for the serodiagnosis of a Coronaviridae infection. On the basis of our results, we propose L. tarentolae as an effective system for viral antigen production, even in countries that lack high-technology cell factories.
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spelling pubmed-87107412021-12-28 Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19 Varotto-Boccazzi, Ilaria Manenti, Alessandro Dapporto, Francesca Gourlay, Louise J. Bisaglia, Beatrice Gabrieli, Paolo Forneris, Federico Faravelli, Silvia Bollati, Valentina Rubolini, Diego Zuccotti, Gianvincenzo Montomoli, Emanuele Epis, Sara Bandi, Claudio Front Microbiol Microbiology To detect and prevent emerging epidemics, discovery platforms are urgently needed, for the rapid development of diagnostic assays. Molecular diagnostic tests for COVID-19 were developed shortly after the isolation of SARS-CoV-2. However, serological tests based on antiviral antibody detection, revealing previous exposure to the virus, required longer testing phases, due to the need to obtain correctly folded and glycosylated antigens. The delay between the identification of a new virus and the development of reliable serodiagnostic tools limits our readiness to tackle future epidemics. We suggest that the protozoan Leishmania tarentolae can be used as an easy-to-handle microfactory for the rapid production of viral antigens to face emerging epidemics. We engineered L. tarentolae to express the SARS-CoV-2 receptor-binding domain (RBD) and we recorded the ability of the purified RBD antigen to detect SARS-CoV-2 infection in human sera, with a sensitivity and reproducibility comparable to that of a reference antigen produced in human cells. This is the first application of an antigen produced in L. tarentolae for the serodiagnosis of a Coronaviridae infection. On the basis of our results, we propose L. tarentolae as an effective system for viral antigen production, even in countries that lack high-technology cell factories. Frontiers Media S.A. 2021-12-13 /pmc/articles/PMC8710741/ /pubmed/34966362 http://dx.doi.org/10.3389/fmicb.2021.736530 Text en Copyright © 2021 Varotto-Boccazzi, Manenti, Dapporto, Gourlay, Bisaglia, Gabrieli, Forneris, Faravelli, Bollati, Rubolini, Zuccotti, Montomoli, Epis and Bandi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Varotto-Boccazzi, Ilaria
Manenti, Alessandro
Dapporto, Francesca
Gourlay, Louise J.
Bisaglia, Beatrice
Gabrieli, Paolo
Forneris, Federico
Faravelli, Silvia
Bollati, Valentina
Rubolini, Diego
Zuccotti, Gianvincenzo
Montomoli, Emanuele
Epis, Sara
Bandi, Claudio
Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19
title Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19
title_full Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19
title_fullStr Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19
title_full_unstemmed Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19
title_short Epidemic Preparedness—Leishmania tarentolae as an Easy-to-Handle Tool to Produce Antigens for Viral Diagnosis: Application to COVID-19
title_sort epidemic preparedness—leishmania tarentolae as an easy-to-handle tool to produce antigens for viral diagnosis: application to covid-19
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8710741/
https://www.ncbi.nlm.nih.gov/pubmed/34966362
http://dx.doi.org/10.3389/fmicb.2021.736530
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