Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19

The utility of the urinary proteome in infectious diseases remains unclear. Here, we analyzed the proteome and metabolome of urine and serum samples from patients with COVID-19 and healthy controls. Our data show that urinary proteins effectively classify COVID-19 by severity. We detect 197 cytokine...

Descripción completa

Detalles Bibliográficos
Autores principales: Bi, Xiaojie, Liu, Wei, Ding, Xuan, Liang, Shuang, Zheng, Yufen, Zhu, Xiaoli, Quan, Sheng, Yi, Xiao, Xiang, Nan, Du, Juping, Lyu, Haiyan, Yu, Die, Zhang, Chao, Xu, Luang, Ge, Weigang, Zhan, Xinke, He, Jiale, Xiong, Zi, Zhang, Shun, Li, Yanchang, Xu, Ping, Zhu, Guangjun, Wang, Donglian, Zhu, Hongguo, Chen, Shiyong, Li, Jun, Zhao, Haihong, Zhu, Yi, Liu, Huafen, Xu, Jiaqin, Shen, Bo, Guo, Tiannan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712267/
https://www.ncbi.nlm.nih.gov/pubmed/35026155
http://dx.doi.org/10.1016/j.celrep.2021.110271
Descripción
Sumario:The utility of the urinary proteome in infectious diseases remains unclear. Here, we analyzed the proteome and metabolome of urine and serum samples from patients with COVID-19 and healthy controls. Our data show that urinary proteins effectively classify COVID-19 by severity. We detect 197 cytokines and their receptors in urine, but only 124 in serum using TMT-based proteomics. The decrease in urinary ESCRT complex proteins correlates with active SARS-CoV-2 replication. The downregulation of urinary CXCL14 in severe COVID-19 cases positively correlates with blood lymphocyte counts. Integrative multiomics analysis suggests that innate immune activation and inflammation triggered renal injuries in patients with COVID-19. COVID-19-associated modulation of the urinary proteome offers unique insights into the pathogenesis of this disease. This study demonstrates the added value of including the urinary proteome in a suite of multiomics analytes in evaluating the immune pathobiology and clinical course of COVID-19 and, potentially, other infectious diseases.

Ejemplares similares