Cargando…
Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19
The utility of the urinary proteome in infectious diseases remains unclear. Here, we analyzed the proteome and metabolome of urine and serum samples from patients with COVID-19 and healthy controls. Our data show that urinary proteins effectively classify COVID-19 by severity. We detect 197 cytokine...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Authors.
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712267/ https://www.ncbi.nlm.nih.gov/pubmed/35026155 http://dx.doi.org/10.1016/j.celrep.2021.110271 |
| _version_ | 1784623530821812224 |
|---|---|
| author | Bi, Xiaojie Liu, Wei Ding, Xuan Liang, Shuang Zheng, Yufen Zhu, Xiaoli Quan, Sheng Yi, Xiao Xiang, Nan Du, Juping Lyu, Haiyan Yu, Die Zhang, Chao Xu, Luang Ge, Weigang Zhan, Xinke He, Jiale Xiong, Zi Zhang, Shun Li, Yanchang Xu, Ping Zhu, Guangjun Wang, Donglian Zhu, Hongguo Chen, Shiyong Li, Jun Zhao, Haihong Zhu, Yi Liu, Huafen Xu, Jiaqin Shen, Bo Guo, Tiannan |
| author_facet | Bi, Xiaojie Liu, Wei Ding, Xuan Liang, Shuang Zheng, Yufen Zhu, Xiaoli Quan, Sheng Yi, Xiao Xiang, Nan Du, Juping Lyu, Haiyan Yu, Die Zhang, Chao Xu, Luang Ge, Weigang Zhan, Xinke He, Jiale Xiong, Zi Zhang, Shun Li, Yanchang Xu, Ping Zhu, Guangjun Wang, Donglian Zhu, Hongguo Chen, Shiyong Li, Jun Zhao, Haihong Zhu, Yi Liu, Huafen Xu, Jiaqin Shen, Bo Guo, Tiannan |
| author_sort | Bi, Xiaojie |
| collection | PubMed |
| description | The utility of the urinary proteome in infectious diseases remains unclear. Here, we analyzed the proteome and metabolome of urine and serum samples from patients with COVID-19 and healthy controls. Our data show that urinary proteins effectively classify COVID-19 by severity. We detect 197 cytokines and their receptors in urine, but only 124 in serum using TMT-based proteomics. The decrease in urinary ESCRT complex proteins correlates with active SARS-CoV-2 replication. The downregulation of urinary CXCL14 in severe COVID-19 cases positively correlates with blood lymphocyte counts. Integrative multiomics analysis suggests that innate immune activation and inflammation triggered renal injuries in patients with COVID-19. COVID-19-associated modulation of the urinary proteome offers unique insights into the pathogenesis of this disease. This study demonstrates the added value of including the urinary proteome in a suite of multiomics analytes in evaluating the immune pathobiology and clinical course of COVID-19 and, potentially, other infectious diseases. |
| format | Online Article Text |
| id | pubmed-8712267 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | The Authors. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-87122672021-12-28 Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19 Bi, Xiaojie Liu, Wei Ding, Xuan Liang, Shuang Zheng, Yufen Zhu, Xiaoli Quan, Sheng Yi, Xiao Xiang, Nan Du, Juping Lyu, Haiyan Yu, Die Zhang, Chao Xu, Luang Ge, Weigang Zhan, Xinke He, Jiale Xiong, Zi Zhang, Shun Li, Yanchang Xu, Ping Zhu, Guangjun Wang, Donglian Zhu, Hongguo Chen, Shiyong Li, Jun Zhao, Haihong Zhu, Yi Liu, Huafen Xu, Jiaqin Shen, Bo Guo, Tiannan Cell Rep Article The utility of the urinary proteome in infectious diseases remains unclear. Here, we analyzed the proteome and metabolome of urine and serum samples from patients with COVID-19 and healthy controls. Our data show that urinary proteins effectively classify COVID-19 by severity. We detect 197 cytokines and their receptors in urine, but only 124 in serum using TMT-based proteomics. The decrease in urinary ESCRT complex proteins correlates with active SARS-CoV-2 replication. The downregulation of urinary CXCL14 in severe COVID-19 cases positively correlates with blood lymphocyte counts. Integrative multiomics analysis suggests that innate immune activation and inflammation triggered renal injuries in patients with COVID-19. COVID-19-associated modulation of the urinary proteome offers unique insights into the pathogenesis of this disease. This study demonstrates the added value of including the urinary proteome in a suite of multiomics analytes in evaluating the immune pathobiology and clinical course of COVID-19 and, potentially, other infectious diseases. The Authors. 2022-01-18 2021-12-28 /pmc/articles/PMC8712267/ /pubmed/35026155 http://dx.doi.org/10.1016/j.celrep.2021.110271 Text en © 2021 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
| spellingShingle | Article Bi, Xiaojie Liu, Wei Ding, Xuan Liang, Shuang Zheng, Yufen Zhu, Xiaoli Quan, Sheng Yi, Xiao Xiang, Nan Du, Juping Lyu, Haiyan Yu, Die Zhang, Chao Xu, Luang Ge, Weigang Zhan, Xinke He, Jiale Xiong, Zi Zhang, Shun Li, Yanchang Xu, Ping Zhu, Guangjun Wang, Donglian Zhu, Hongguo Chen, Shiyong Li, Jun Zhao, Haihong Zhu, Yi Liu, Huafen Xu, Jiaqin Shen, Bo Guo, Tiannan Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19 |
| title | Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19 |
| title_full | Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19 |
| title_fullStr | Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19 |
| title_full_unstemmed | Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19 |
| title_short | Proteomic and metabolomic profiling of urine uncovers immune responses in patients with COVID-19 |
| title_sort | proteomic and metabolomic profiling of urine uncovers immune responses in patients with covid-19 |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712267/ https://www.ncbi.nlm.nih.gov/pubmed/35026155 http://dx.doi.org/10.1016/j.celrep.2021.110271 |
| work_keys_str_mv | AT bixiaojie proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT liuwei proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT dingxuan proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT liangshuang proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhengyufen proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhuxiaoli proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT quansheng proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT yixiao proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT xiangnan proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT dujuping proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT lyuhaiyan proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT yudie proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhangchao proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT xuluang proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT geweigang proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhanxinke proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT hejiale proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT xiongzi proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhangshun proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT liyanchang proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT xuping proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhuguangjun proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT wangdonglian proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhuhongguo proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT chenshiyong proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT lijun proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhaohaihong proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT zhuyi proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT liuhuafen proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT xujiaqin proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT shenbo proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 AT guotiannan proteomicandmetabolomicprofilingofurineuncoversimmuneresponsesinpatientswithcovid19 |