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Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB)

Hydroxyl-terminated polybutadiene (HTPB) is a curing adhesive that is commonly used in the production of ammunition, and it emerged during the time of war. After entering the peaceful era, several countries around the globe have focused on the destruction of expired ammunition using safe and economi...

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Autores principales: Zhang, Ying, Zou, Min, Lodhi, Adil Farooq, Deng, Yu-lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712286/
https://www.ncbi.nlm.nih.gov/pubmed/34958447
http://dx.doi.org/10.1186/s13568-021-01334-1
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author Zhang, Ying
Zou, Min
Lodhi, Adil Farooq
Deng, Yu-lin
author_facet Zhang, Ying
Zou, Min
Lodhi, Adil Farooq
Deng, Yu-lin
author_sort Zhang, Ying
collection PubMed
description Hydroxyl-terminated polybutadiene (HTPB) is a curing adhesive that is commonly used in the production of ammunition, and it emerged during the time of war. After entering the peaceful era, several countries around the globe have focused on the destruction of expired ammunition using safe and economical methods in terms of consumption of energy. Microorganisms exhibit a highly efficient and environment friendly degradation capability for variety of refractory substances. Therefore, in this study we screened five strains of microorganisms from five environmental soil samples for their ability to degrade HTPB. These microorganisms were identified as Microbacterium trichothecenolyticum, Microbacterium esteraromaticum, Arthrobacter pascens, Pseudonocardia carboxydivorans and Ochrobactrum anthropic based on 16S rRNA gene similarity index. We observed the uncorroded and corroded HTPB sample through scanning electron microscopy and observed the formation of lot of holes and gullies in HTPB after corrosion. An 18S rRNA gene clone library was constructed for HTPB-degrading fungi. Based on the results of library evaluation, it was found that the structure of the HTPB-degrading fungi community was relatively simple. A total of 54 positive clones were obtained. These clones represented some uncultured microorganisms that were closely related to Scytalidium lignicola, Pseudokahliella and Gonostomum strenuum. This study will help in the implementation of environment friendly degradation strategies for HTPB degradation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01334-1.
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spelling pubmed-87122862022-01-10 Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB) Zhang, Ying Zou, Min Lodhi, Adil Farooq Deng, Yu-lin AMB Express Original Article Hydroxyl-terminated polybutadiene (HTPB) is a curing adhesive that is commonly used in the production of ammunition, and it emerged during the time of war. After entering the peaceful era, several countries around the globe have focused on the destruction of expired ammunition using safe and economical methods in terms of consumption of energy. Microorganisms exhibit a highly efficient and environment friendly degradation capability for variety of refractory substances. Therefore, in this study we screened five strains of microorganisms from five environmental soil samples for their ability to degrade HTPB. These microorganisms were identified as Microbacterium trichothecenolyticum, Microbacterium esteraromaticum, Arthrobacter pascens, Pseudonocardia carboxydivorans and Ochrobactrum anthropic based on 16S rRNA gene similarity index. We observed the uncorroded and corroded HTPB sample through scanning electron microscopy and observed the formation of lot of holes and gullies in HTPB after corrosion. An 18S rRNA gene clone library was constructed for HTPB-degrading fungi. Based on the results of library evaluation, it was found that the structure of the HTPB-degrading fungi community was relatively simple. A total of 54 positive clones were obtained. These clones represented some uncultured microorganisms that were closely related to Scytalidium lignicola, Pseudokahliella and Gonostomum strenuum. This study will help in the implementation of environment friendly degradation strategies for HTPB degradation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13568-021-01334-1. Springer Berlin Heidelberg 2021-12-27 /pmc/articles/PMC8712286/ /pubmed/34958447 http://dx.doi.org/10.1186/s13568-021-01334-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Zhang, Ying
Zou, Min
Lodhi, Adil Farooq
Deng, Yu-lin
Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB)
title Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB)
title_full Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB)
title_fullStr Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB)
title_full_unstemmed Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB)
title_short Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB)
title_sort microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (htpb)
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712286/
https://www.ncbi.nlm.nih.gov/pubmed/34958447
http://dx.doi.org/10.1186/s13568-021-01334-1
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