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Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco
Bacterial wilt (BW) caused by Ralstonia solanacearum (R. solanacearum), is a vascular disease affecting diverse solanaceous crops and causing tremendous damage to crop production. However, our knowledge of the mechanism underlying its resistance or susceptibility is very limited. In this study, we c...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712766/ https://www.ncbi.nlm.nih.gov/pubmed/34970284 http://dx.doi.org/10.3389/fpls.2021.767882 |
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author | Pan, Xiaoying Chen, Junbiao Yang, Aiguo Yuan, Qinghua Zhao, Weicai Xu, Tingyu Chen, Bowen Ren, Min Geng, Ruimei Zong, Zhaohui Ma, Zhuwen Huang, Zhenrui Zhang, Zhenchen |
author_facet | Pan, Xiaoying Chen, Junbiao Yang, Aiguo Yuan, Qinghua Zhao, Weicai Xu, Tingyu Chen, Bowen Ren, Min Geng, Ruimei Zong, Zhaohui Ma, Zhuwen Huang, Zhenrui Zhang, Zhenchen |
author_sort | Pan, Xiaoying |
collection | PubMed |
description | Bacterial wilt (BW) caused by Ralstonia solanacearum (R. solanacearum), is a vascular disease affecting diverse solanaceous crops and causing tremendous damage to crop production. However, our knowledge of the mechanism underlying its resistance or susceptibility is very limited. In this study, we characterized the physiological differences and compared the defense-related transcriptomes of two tobacco varieties, 4411-3 (highly resistant, HR) and K326 (moderately resistant, MR), after R. solanacearum infection at 0, 10, and 17 days after inoculation (dpi). A total of 3967 differentially expressed genes (DEGs) were identified between the HR and MR genotypes under mock condition at three time points, including1395 up-regulated genes in the HR genotype and 2640 up-regulated genes in the MR genotype. Also, 6,233 and 21,541 DEGs were induced in the HR and MR genotypes after R. solanacearum infection, respectively. Furthermore, GO and KEGG analyses revealed that DEGs in the HR genotype were related to the cell wall, starch and sucrose metabolism, glutathione metabolism, ABC transporters, endocytosis, glycerolipid metabolism, and glycerophospholipid metabolism. The defense-related genes generally showed genotype-specific regulation and expression differences after R. solanacearum infection. In addition, genes related to auxin and ABA were dramatically up-regulated in the HR genotype. The contents of auxin and ABA in the MR genotype were significantly higher than those in the HR genotype after R. solanacearum infection, providing insight into the defense mechanisms of tobacco. Altogether, these results clarify the physiological and transcriptional regulation of R. solanacearum resistance infection in tobacco, and improve our understanding of the molecular mechanism underlying the plant-pathogen interaction. |
format | Online Article Text |
id | pubmed-8712766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87127662021-12-29 Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco Pan, Xiaoying Chen, Junbiao Yang, Aiguo Yuan, Qinghua Zhao, Weicai Xu, Tingyu Chen, Bowen Ren, Min Geng, Ruimei Zong, Zhaohui Ma, Zhuwen Huang, Zhenrui Zhang, Zhenchen Front Plant Sci Plant Science Bacterial wilt (BW) caused by Ralstonia solanacearum (R. solanacearum), is a vascular disease affecting diverse solanaceous crops and causing tremendous damage to crop production. However, our knowledge of the mechanism underlying its resistance or susceptibility is very limited. In this study, we characterized the physiological differences and compared the defense-related transcriptomes of two tobacco varieties, 4411-3 (highly resistant, HR) and K326 (moderately resistant, MR), after R. solanacearum infection at 0, 10, and 17 days after inoculation (dpi). A total of 3967 differentially expressed genes (DEGs) were identified between the HR and MR genotypes under mock condition at three time points, including1395 up-regulated genes in the HR genotype and 2640 up-regulated genes in the MR genotype. Also, 6,233 and 21,541 DEGs were induced in the HR and MR genotypes after R. solanacearum infection, respectively. Furthermore, GO and KEGG analyses revealed that DEGs in the HR genotype were related to the cell wall, starch and sucrose metabolism, glutathione metabolism, ABC transporters, endocytosis, glycerolipid metabolism, and glycerophospholipid metabolism. The defense-related genes generally showed genotype-specific regulation and expression differences after R. solanacearum infection. In addition, genes related to auxin and ABA were dramatically up-regulated in the HR genotype. The contents of auxin and ABA in the MR genotype were significantly higher than those in the HR genotype after R. solanacearum infection, providing insight into the defense mechanisms of tobacco. Altogether, these results clarify the physiological and transcriptional regulation of R. solanacearum resistance infection in tobacco, and improve our understanding of the molecular mechanism underlying the plant-pathogen interaction. Frontiers Media S.A. 2021-12-14 /pmc/articles/PMC8712766/ /pubmed/34970284 http://dx.doi.org/10.3389/fpls.2021.767882 Text en Copyright © 2021 Pan, Chen, Yang, Yuan, Zhao, Xu, Chen, Ren, Geng, Zong, Ma, Huang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Pan, Xiaoying Chen, Junbiao Yang, Aiguo Yuan, Qinghua Zhao, Weicai Xu, Tingyu Chen, Bowen Ren, Min Geng, Ruimei Zong, Zhaohui Ma, Zhuwen Huang, Zhenrui Zhang, Zhenchen Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco |
title | Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco |
title_full | Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco |
title_fullStr | Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco |
title_full_unstemmed | Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco |
title_short | Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco |
title_sort | comparative transcriptome profiling reveals defense-related genes against ralstonia solanacearum infection in tobacco |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8712766/ https://www.ncbi.nlm.nih.gov/pubmed/34970284 http://dx.doi.org/10.3389/fpls.2021.767882 |
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