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Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing

Since the Coronavirus 19 (COVID-19) pandemic, several SARS-CoV-2 variants of concern (SARS-CoV-2 VOC) have been reported. The B.1.1.7 variant has been associated with increased mortality and transmission risk. Furthermore, cluster and possible co-infection cases could occur in the next influenza sea...

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Autores principales: Chung, Hsing-Yi, Jian, Ming-Jr, Chang, Chih-Kai, Lin, Jung-Chung, Yeh, Kuo-Ming, Yang, Ya-Sung, Chen, Chien-Wen, Hsieh, Shan-Shan, Tang, Sheng-Hui, Perng, Cherng-Lih, Chang, Feng-Yee, Hung, Kuo-Sheng, Chen, En-Sung, Yang, Mei-Hsiu, Shang, Hung-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714143/
https://www.ncbi.nlm.nih.gov/pubmed/34897035
http://dx.doi.org/10.18632/aging.203761
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author Chung, Hsing-Yi
Jian, Ming-Jr
Chang, Chih-Kai
Lin, Jung-Chung
Yeh, Kuo-Ming
Yang, Ya-Sung
Chen, Chien-Wen
Hsieh, Shan-Shan
Tang, Sheng-Hui
Perng, Cherng-Lih
Chang, Feng-Yee
Hung, Kuo-Sheng
Chen, En-Sung
Yang, Mei-Hsiu
Shang, Hung-Sheng
author_facet Chung, Hsing-Yi
Jian, Ming-Jr
Chang, Chih-Kai
Lin, Jung-Chung
Yeh, Kuo-Ming
Yang, Ya-Sung
Chen, Chien-Wen
Hsieh, Shan-Shan
Tang, Sheng-Hui
Perng, Cherng-Lih
Chang, Feng-Yee
Hung, Kuo-Sheng
Chen, En-Sung
Yang, Mei-Hsiu
Shang, Hung-Sheng
author_sort Chung, Hsing-Yi
collection PubMed
description Since the Coronavirus 19 (COVID-19) pandemic, several SARS-CoV-2 variants of concern (SARS-CoV-2 VOC) have been reported. The B.1.1.7 variant has been associated with increased mortality and transmission risk. Furthermore, cluster and possible co-infection cases could occur in the next influenza season or COVID-19 pandemic wave, warranting efficient diagnosis and treatment decision making. Here, we aimed to detect SARS-CoV-2 and other common respiratory viruses using multiplex RT-PCR developed on the LabTurbo AIO 48 open system. We performed a multicenter study to evaluate the performance and analytical sensitivity of the LabTurbo AIO 48 system for SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) using 652 nasopharyngeal swab clinical samples from patients. The LabTurbo AIO 48 system demonstrated a sensitivity of 9.4 copies/per PCR for N2 of SARS-CoV-2; 24 copies/per PCR for M of influenza A and B; and 24 copies/per PCR for N of RSV. The assay presented consistent performance in the multicenter study. The multiplex RT-PCR applied on the LabTurbo AIO 48 open platform provided highly sensitive, robust, and accurate results and enabled high-throughput detection of B.1.1.7, influenza A/B, and RSV with short turnaround times. Therefore, this automated molecular diagnostic assay could enable streamlined testing if COVID-19 becomes a seasonal disease.
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spelling pubmed-87141432021-12-29 Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing Chung, Hsing-Yi Jian, Ming-Jr Chang, Chih-Kai Lin, Jung-Chung Yeh, Kuo-Ming Yang, Ya-Sung Chen, Chien-Wen Hsieh, Shan-Shan Tang, Sheng-Hui Perng, Cherng-Lih Chang, Feng-Yee Hung, Kuo-Sheng Chen, En-Sung Yang, Mei-Hsiu Shang, Hung-Sheng Aging (Albany NY) Research Paper Since the Coronavirus 19 (COVID-19) pandemic, several SARS-CoV-2 variants of concern (SARS-CoV-2 VOC) have been reported. The B.1.1.7 variant has been associated with increased mortality and transmission risk. Furthermore, cluster and possible co-infection cases could occur in the next influenza season or COVID-19 pandemic wave, warranting efficient diagnosis and treatment decision making. Here, we aimed to detect SARS-CoV-2 and other common respiratory viruses using multiplex RT-PCR developed on the LabTurbo AIO 48 open system. We performed a multicenter study to evaluate the performance and analytical sensitivity of the LabTurbo AIO 48 system for SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) using 652 nasopharyngeal swab clinical samples from patients. The LabTurbo AIO 48 system demonstrated a sensitivity of 9.4 copies/per PCR for N2 of SARS-CoV-2; 24 copies/per PCR for M of influenza A and B; and 24 copies/per PCR for N of RSV. The assay presented consistent performance in the multicenter study. The multiplex RT-PCR applied on the LabTurbo AIO 48 open platform provided highly sensitive, robust, and accurate results and enabled high-throughput detection of B.1.1.7, influenza A/B, and RSV with short turnaround times. Therefore, this automated molecular diagnostic assay could enable streamlined testing if COVID-19 becomes a seasonal disease. Impact Journals 2021-12-12 /pmc/articles/PMC8714143/ /pubmed/34897035 http://dx.doi.org/10.18632/aging.203761 Text en Copyright: © 2021 Chung et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chung, Hsing-Yi
Jian, Ming-Jr
Chang, Chih-Kai
Lin, Jung-Chung
Yeh, Kuo-Ming
Yang, Ya-Sung
Chen, Chien-Wen
Hsieh, Shan-Shan
Tang, Sheng-Hui
Perng, Cherng-Lih
Chang, Feng-Yee
Hung, Kuo-Sheng
Chen, En-Sung
Yang, Mei-Hsiu
Shang, Hung-Sheng
Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
title Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
title_full Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
title_fullStr Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
title_full_unstemmed Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
title_short Multicenter study evaluating one multiplex RT-PCR assay to detect SARS-CoV-2, influenza A/B, and respiratory syncytia virus using the LabTurbo AIO open platform: epidemiological features, automated sample-to-result, and high-throughput testing
title_sort multicenter study evaluating one multiplex rt-pcr assay to detect sars-cov-2, influenza a/b, and respiratory syncytia virus using the labturbo aio open platform: epidemiological features, automated sample-to-result, and high-throughput testing
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714143/
https://www.ncbi.nlm.nih.gov/pubmed/34897035
http://dx.doi.org/10.18632/aging.203761
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