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Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress

Autotoxicity is known as a critical factor in replanting problem that reduces land utilization and creates economic losses. Benzoic acid (BA) is identified as a major autotoxin in peach replant problem, and causes stunted seedling growth or even death. However, the physiological and molecular mechan...

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Autores principales: Shen, Wanqi, Zeng, Chunfa, Zhang, He, Zhu, Kaijie, He, Hao, Zhu, Wei, He, Hanzi, Li, Guohuai, Liu, Junwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714634/
https://www.ncbi.nlm.nih.gov/pubmed/34975982
http://dx.doi.org/10.3389/fpls.2021.794881
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author Shen, Wanqi
Zeng, Chunfa
Zhang, He
Zhu, Kaijie
He, Hao
Zhu, Wei
He, Hanzi
Li, Guohuai
Liu, Junwei
author_facet Shen, Wanqi
Zeng, Chunfa
Zhang, He
Zhu, Kaijie
He, Hao
Zhu, Wei
He, Hanzi
Li, Guohuai
Liu, Junwei
author_sort Shen, Wanqi
collection PubMed
description Autotoxicity is known as a critical factor in replanting problem that reduces land utilization and creates economic losses. Benzoic acid (BA) is identified as a major autotoxin in peach replant problem, and causes stunted seedling growth or even death. However, the physiological and molecular mechanisms of peach response to BA stress remain elusive. Here, we comprehensively studied the morphophysiological, transcriptional, and metabolic responses of peach plants to BA toxicity. Results showed that BA stress inhibited peach seedlings growth, decreased chlorophyll contents and fluorescence levels, as well as disturbed mineral metabolism. The contents of hydrogen peroxide, superoxide anion, and malondialdehyde, as well as the total antioxidant capacity, were significantly increased under BA stress. A total of 6,319 differentially expressed genes (DEGs) were identified after BA stress, of which the DEGs related to photosynthesis, redox, and ion metabolism were greatly changed; meanwhile, numerous stress-responsive genes (HSPs, GSTs, GR, and ABC transporters) and transcription factors (MYB, AP2/ERF, NAC, bHLH, and WRKY) were noticeably altered under BA stress. BA induced metabolic reprogramming, and 74 differentially accumulated metabolites, including amino acids and derivatives, fatty acids, organic acids, sugars, and sugar alcohols, were identified in BA-stressed roots. Furthermore, an integrated analysis of genes and metabolites indicated that most of the co-mapped KEGG pathways were enriched in amino acid and carbohydrate metabolism, which implied a disturbed carbon and nitrogen metabolism after BA stress. The findings would be insightful in elucidating the mechanisms of plant response to autotoxicity stress, and help guide crops in alleviating replant problem.
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spelling pubmed-87146342021-12-30 Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress Shen, Wanqi Zeng, Chunfa Zhang, He Zhu, Kaijie He, Hao Zhu, Wei He, Hanzi Li, Guohuai Liu, Junwei Front Plant Sci Plant Science Autotoxicity is known as a critical factor in replanting problem that reduces land utilization and creates economic losses. Benzoic acid (BA) is identified as a major autotoxin in peach replant problem, and causes stunted seedling growth or even death. However, the physiological and molecular mechanisms of peach response to BA stress remain elusive. Here, we comprehensively studied the morphophysiological, transcriptional, and metabolic responses of peach plants to BA toxicity. Results showed that BA stress inhibited peach seedlings growth, decreased chlorophyll contents and fluorescence levels, as well as disturbed mineral metabolism. The contents of hydrogen peroxide, superoxide anion, and malondialdehyde, as well as the total antioxidant capacity, were significantly increased under BA stress. A total of 6,319 differentially expressed genes (DEGs) were identified after BA stress, of which the DEGs related to photosynthesis, redox, and ion metabolism were greatly changed; meanwhile, numerous stress-responsive genes (HSPs, GSTs, GR, and ABC transporters) and transcription factors (MYB, AP2/ERF, NAC, bHLH, and WRKY) were noticeably altered under BA stress. BA induced metabolic reprogramming, and 74 differentially accumulated metabolites, including amino acids and derivatives, fatty acids, organic acids, sugars, and sugar alcohols, were identified in BA-stressed roots. Furthermore, an integrated analysis of genes and metabolites indicated that most of the co-mapped KEGG pathways were enriched in amino acid and carbohydrate metabolism, which implied a disturbed carbon and nitrogen metabolism after BA stress. The findings would be insightful in elucidating the mechanisms of plant response to autotoxicity stress, and help guide crops in alleviating replant problem. Frontiers Media S.A. 2021-12-15 /pmc/articles/PMC8714634/ /pubmed/34975982 http://dx.doi.org/10.3389/fpls.2021.794881 Text en Copyright © 2021 Shen, Zeng, Zhang, Zhu, He, Zhu, He, Li and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Shen, Wanqi
Zeng, Chunfa
Zhang, He
Zhu, Kaijie
He, Hao
Zhu, Wei
He, Hanzi
Li, Guohuai
Liu, Junwei
Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress
title Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress
title_full Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress
title_fullStr Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress
title_full_unstemmed Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress
title_short Integrative Physiological, Transcriptional, and Metabolic Analyses Provide Insights Into Response Mechanisms of Prunus persica to Autotoxicity Stress
title_sort integrative physiological, transcriptional, and metabolic analyses provide insights into response mechanisms of prunus persica to autotoxicity stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714634/
https://www.ncbi.nlm.nih.gov/pubmed/34975982
http://dx.doi.org/10.3389/fpls.2021.794881
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