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Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis

MYBs (v-myb avian myeloblastosis viral oncogene homologs) are important transcriptional regulators that play critical roles in the regulation of anthocyanin biosynthesis. The overexpression of MYB genes has been reported in different plant species. However, the inconsistent strategies to assess tran...

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Autores principales: Liu, Weichao, Zheng, Tangchun, Yang, Yujie, Li, Ping, Qiu, Like, Li, Lulu, Wang, Jia, Cheng, Tangren, Zhang, Qixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714666/
https://www.ncbi.nlm.nih.gov/pubmed/34975967
http://dx.doi.org/10.3389/fpls.2021.781343
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author Liu, Weichao
Zheng, Tangchun
Yang, Yujie
Li, Ping
Qiu, Like
Li, Lulu
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
author_facet Liu, Weichao
Zheng, Tangchun
Yang, Yujie
Li, Ping
Qiu, Like
Li, Lulu
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
author_sort Liu, Weichao
collection PubMed
description MYBs (v-myb avian myeloblastosis viral oncogene homologs) are important transcriptional regulators that play critical roles in the regulation of anthocyanin biosynthesis. The overexpression of MYB genes has been reported in different plant species. However, the inconsistent strategies to assess transgenic plants have made it difficult to explain the complex mechanisms of regulation of anthocyanin biosynthesis by MYBs. We report here a meta-analysis of 608 studies from 206 publications assessing the effects of MYB overexpression on anthocyanins and evaluate the experimental variables that have an influence on transgenic plant performance. We found that MYB expression enhanced the magnitude of 20 out of 26 examined plant parameters by at least of 21% and reduced the magnitude of 1 indicator by at least 37%. We explored the variety of moderating variables causing these variations. A deeper color induced by MYBs caused higher plant attributes as compared to normal color changes. MYB genes from dicots stimulated the accumulation of anthocyanins, flavonols and impacted the expressions of PAL, CHS, CHI, FLS, F3′5′H, ANS, UFGT, and ANR as compared to monocots. Heterologous expression and homologous expression showed a great difference in anthocyanin biosynthesis. Transient gene transformation had a significant effect on the expression of flavonoid biosynthetic genes, and stable transformation had a significant effect on flavonoid accumulation. Stress could result in a significantly increased accumulation of flavonoids, especially anthocyanin, flavonol, and proanthocyanidin. Our study, thus, provides new insights into the function of MYBs in the regulatory mechanisms of flavonoid biosynthesis and the use of genetic engineering for improving anthocyanins contents.
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spelling pubmed-87146662021-12-30 Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis Liu, Weichao Zheng, Tangchun Yang, Yujie Li, Ping Qiu, Like Li, Lulu Wang, Jia Cheng, Tangren Zhang, Qixiang Front Plant Sci Plant Science MYBs (v-myb avian myeloblastosis viral oncogene homologs) are important transcriptional regulators that play critical roles in the regulation of anthocyanin biosynthesis. The overexpression of MYB genes has been reported in different plant species. However, the inconsistent strategies to assess transgenic plants have made it difficult to explain the complex mechanisms of regulation of anthocyanin biosynthesis by MYBs. We report here a meta-analysis of 608 studies from 206 publications assessing the effects of MYB overexpression on anthocyanins and evaluate the experimental variables that have an influence on transgenic plant performance. We found that MYB expression enhanced the magnitude of 20 out of 26 examined plant parameters by at least of 21% and reduced the magnitude of 1 indicator by at least 37%. We explored the variety of moderating variables causing these variations. A deeper color induced by MYBs caused higher plant attributes as compared to normal color changes. MYB genes from dicots stimulated the accumulation of anthocyanins, flavonols and impacted the expressions of PAL, CHS, CHI, FLS, F3′5′H, ANS, UFGT, and ANR as compared to monocots. Heterologous expression and homologous expression showed a great difference in anthocyanin biosynthesis. Transient gene transformation had a significant effect on the expression of flavonoid biosynthetic genes, and stable transformation had a significant effect on flavonoid accumulation. Stress could result in a significantly increased accumulation of flavonoids, especially anthocyanin, flavonol, and proanthocyanidin. Our study, thus, provides new insights into the function of MYBs in the regulatory mechanisms of flavonoid biosynthesis and the use of genetic engineering for improving anthocyanins contents. Frontiers Media S.A. 2021-12-15 /pmc/articles/PMC8714666/ /pubmed/34975967 http://dx.doi.org/10.3389/fpls.2021.781343 Text en Copyright © 2021 Liu, Zheng, Yang, Li, Qiu, Li, Wang, Cheng and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Weichao
Zheng, Tangchun
Yang, Yujie
Li, Ping
Qiu, Like
Li, Lulu
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis
title Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis
title_full Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis
title_fullStr Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis
title_full_unstemmed Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis
title_short Meta-Analysis of the Effect of Overexpression of MYB Transcription Factors on the Regulatory Mechanisms of Anthocyanin Biosynthesis
title_sort meta-analysis of the effect of overexpression of myb transcription factors on the regulatory mechanisms of anthocyanin biosynthesis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714666/
https://www.ncbi.nlm.nih.gov/pubmed/34975967
http://dx.doi.org/10.3389/fpls.2021.781343
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