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YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma

Abnormal expression of the transcription factor Y-box-binding protein-1 (YBX1) is associated with the proliferation, migration, aggressiveness, and stem-like properties of various cancers. These characteristics contribute to the tumorigenesis and metastasis of cancer. We found that the expression le...

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Autores principales: Xie, Qiang, Zhao, Shilei, Liu, Wenzhi, Cui, Yanwei, Li, Fengzhou, Li, Zhuoshi, Guo, Tao, Yu, Wendan, Guo, Wei, Deng, Wuguo, Gu, Chundong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714800/
https://www.ncbi.nlm.nih.gov/pubmed/34976785
http://dx.doi.org/10.3389/fonc.2021.702491
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author Xie, Qiang
Zhao, Shilei
Liu, Wenzhi
Cui, Yanwei
Li, Fengzhou
Li, Zhuoshi
Guo, Tao
Yu, Wendan
Guo, Wei
Deng, Wuguo
Gu, Chundong
author_facet Xie, Qiang
Zhao, Shilei
Liu, Wenzhi
Cui, Yanwei
Li, Fengzhou
Li, Zhuoshi
Guo, Tao
Yu, Wendan
Guo, Wei
Deng, Wuguo
Gu, Chundong
author_sort Xie, Qiang
collection PubMed
description Abnormal expression of the transcription factor Y-box-binding protein-1 (YBX1) is associated with the proliferation, migration, aggressiveness, and stem-like properties of various cancers. These characteristics contribute to the tumorigenesis and metastasis of cancer. We found that the expression levels of Mucin-1 (MUC1) and YBX1 were positively correlated in lung adenocarcinoma cells and lung adenocarcinoma tissue. Our retrospective cohort study of 176 lung adenocarcinoma patients after surgery showed that low expression of both YBX1 and MUC1 was an independent predictor of the prognosis and recurrence of lung adenocarcinoma. In lung adenocarcinoma cells, the silencing/overexpression of YBX1 caused a simultaneous change in MUC1, and MUC1 overexpression partially reversed the decreased tumor cell migration, aggressiveness, and stemness caused by YBX1 silencing. Moreover, chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays proved that MUC1 was the downstream target of YBX1 and that YBX1 bound to the -1480~-1476 position in the promoter region of MUC1 to regulate its transcription. Furthermore, in mouse xenograft models and a lung cancer metastasis model, MUC1, which is downstream of YBX1, partially reversed the decreased number and size of tumors caused by YBX1 silencing. In conclusion, our findings indicated a novel mechanism by which YBX1 promotes the stemness and metastasis of lung adenocarcinoma by targeting MUC1 and provided a combination approach for diagnosis different from traditional single tumor biomarkers to predict patient prognosis and provide clinical treatment targets.
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spelling pubmed-87148002021-12-30 YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma Xie, Qiang Zhao, Shilei Liu, Wenzhi Cui, Yanwei Li, Fengzhou Li, Zhuoshi Guo, Tao Yu, Wendan Guo, Wei Deng, Wuguo Gu, Chundong Front Oncol Oncology Abnormal expression of the transcription factor Y-box-binding protein-1 (YBX1) is associated with the proliferation, migration, aggressiveness, and stem-like properties of various cancers. These characteristics contribute to the tumorigenesis and metastasis of cancer. We found that the expression levels of Mucin-1 (MUC1) and YBX1 were positively correlated in lung adenocarcinoma cells and lung adenocarcinoma tissue. Our retrospective cohort study of 176 lung adenocarcinoma patients after surgery showed that low expression of both YBX1 and MUC1 was an independent predictor of the prognosis and recurrence of lung adenocarcinoma. In lung adenocarcinoma cells, the silencing/overexpression of YBX1 caused a simultaneous change in MUC1, and MUC1 overexpression partially reversed the decreased tumor cell migration, aggressiveness, and stemness caused by YBX1 silencing. Moreover, chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays proved that MUC1 was the downstream target of YBX1 and that YBX1 bound to the -1480~-1476 position in the promoter region of MUC1 to regulate its transcription. Furthermore, in mouse xenograft models and a lung cancer metastasis model, MUC1, which is downstream of YBX1, partially reversed the decreased number and size of tumors caused by YBX1 silencing. In conclusion, our findings indicated a novel mechanism by which YBX1 promotes the stemness and metastasis of lung adenocarcinoma by targeting MUC1 and provided a combination approach for diagnosis different from traditional single tumor biomarkers to predict patient prognosis and provide clinical treatment targets. Frontiers Media S.A. 2021-12-15 /pmc/articles/PMC8714800/ /pubmed/34976785 http://dx.doi.org/10.3389/fonc.2021.702491 Text en Copyright © 2021 Xie, Zhao, Liu, Cui, Li, Li, Guo, Yu, Guo, Deng and Gu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Xie, Qiang
Zhao, Shilei
Liu, Wenzhi
Cui, Yanwei
Li, Fengzhou
Li, Zhuoshi
Guo, Tao
Yu, Wendan
Guo, Wei
Deng, Wuguo
Gu, Chundong
YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma
title YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma
title_full YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma
title_fullStr YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma
title_full_unstemmed YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma
title_short YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma
title_sort ybx1 enhances metastasis and stemness by transcriptionally regulating muc1 in lung adenocarcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714800/
https://www.ncbi.nlm.nih.gov/pubmed/34976785
http://dx.doi.org/10.3389/fonc.2021.702491
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