Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex

Host tRNAs specifically interact with the matrix domain (MA) of HIV-1 major structural polyprotein, Gag, to control its membrane localization and virion assembly. In this protocol, we describe the purification and engineering of HIV-1 MA and tRNA, and the co-crystallization and structure determinati...

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Detalles Bibliográficos
Autores principales: Bou-Nader, Charles, Zhang, Jinwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715211/
https://www.ncbi.nlm.nih.gov/pubmed/35005638
http://dx.doi.org/10.1016/j.xpro.2021.101056

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715211/
https://www.ncbi.nlm.nih.gov/pubmed/35005638
http://dx.doi.org/10.1016/j.xpro.2021.101056

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