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Rational engineering enables co-crystallization and structural determination of the HIV-1 matrix-tRNA complex
Host tRNAs specifically interact with the matrix domain (MA) of HIV-1 major structural polyprotein, Gag, to control its membrane localization and virion assembly. In this protocol, we describe the purification and engineering of HIV-1 MA and tRNA, and the co-crystallization and structure determinati...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715211/ https://www.ncbi.nlm.nih.gov/pubmed/35005638 http://dx.doi.org/10.1016/j.xpro.2021.101056 |