Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography

Following myocardial infarction, damaged myocardium is replaced with a fibrotic scar that preserves cardiac structural integrity. Scar area measured from sample 2D images of serial heart sections does not faithfully measure the extent of fibrosis due to structural heterogeneity caused by tissue dyna...

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Detalles Bibliográficos
Autores principales: Janbandhu, Vaibhao, Martin, Ella M.M.A., Chapman, Gavin, Dunwoodie, Sally L., Harvey, Richard P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715218/
https://www.ncbi.nlm.nih.gov/pubmed/35005637
http://dx.doi.org/10.1016/j.xpro.2021.101055
Descripción
Sumario:Following myocardial infarction, damaged myocardium is replaced with a fibrotic scar that preserves cardiac structural integrity. Scar area measured from sample 2D images of serial heart sections does not faithfully measure the extent of fibrosis due to structural heterogeneity caused by tissue dynamics. Here, we present an X-ray microcomputed tomography (micro-CT) workflow that generates accurate volumetric quantification of scar and surviving myocardium in infarcted mouse hearts. This workflow could be applied to other fibrotic organs or hearts from other species. For complete details on the use and execution of this protocol, please refer to Janbandhu et al. (2021).

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