Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography

Following myocardial infarction, damaged myocardium is replaced with a fibrotic scar that preserves cardiac structural integrity. Scar area measured from sample 2D images of serial heart sections does not faithfully measure the extent of fibrosis due to structural heterogeneity caused by tissue dyna...

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Detalles Bibliográficos
Autores principales: Janbandhu, Vaibhao, Martin, Ella M.M.A., Chapman, Gavin, Dunwoodie, Sally L., Harvey, Richard P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715218/
https://www.ncbi.nlm.nih.gov/pubmed/35005637
http://dx.doi.org/10.1016/j.xpro.2021.101055
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author Janbandhu, Vaibhao
Martin, Ella M.M.A.
Chapman, Gavin
Dunwoodie, Sally L.
Harvey, Richard P.
author_facet Janbandhu, Vaibhao
Martin, Ella M.M.A.
Chapman, Gavin
Dunwoodie, Sally L.
Harvey, Richard P.
author_sort Janbandhu, Vaibhao
collection PubMed
description Following myocardial infarction, damaged myocardium is replaced with a fibrotic scar that preserves cardiac structural integrity. Scar area measured from sample 2D images of serial heart sections does not faithfully measure the extent of fibrosis due to structural heterogeneity caused by tissue dynamics. Here, we present an X-ray microcomputed tomography (micro-CT) workflow that generates accurate volumetric quantification of scar and surviving myocardium in infarcted mouse hearts. This workflow could be applied to other fibrotic organs or hearts from other species. For complete details on the use and execution of this protocol, please refer to Janbandhu et al. (2021).
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spelling pubmed-87152182022-01-06 Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography Janbandhu, Vaibhao Martin, Ella M.M.A. Chapman, Gavin Dunwoodie, Sally L. Harvey, Richard P. STAR Protoc Protocol Following myocardial infarction, damaged myocardium is replaced with a fibrotic scar that preserves cardiac structural integrity. Scar area measured from sample 2D images of serial heart sections does not faithfully measure the extent of fibrosis due to structural heterogeneity caused by tissue dynamics. Here, we present an X-ray microcomputed tomography (micro-CT) workflow that generates accurate volumetric quantification of scar and surviving myocardium in infarcted mouse hearts. This workflow could be applied to other fibrotic organs or hearts from other species. For complete details on the use and execution of this protocol, please refer to Janbandhu et al. (2021). Elsevier 2021-12-22 /pmc/articles/PMC8715218/ /pubmed/35005637 http://dx.doi.org/10.1016/j.xpro.2021.101055 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Janbandhu, Vaibhao
Martin, Ella M.M.A.
Chapman, Gavin
Dunwoodie, Sally L.
Harvey, Richard P.
Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
title Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
title_full Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
title_fullStr Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
title_full_unstemmed Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
title_short Quantitative 3D analysis and visualization of cardiac fibrosis by microcomputed tomography
title_sort quantitative 3d analysis and visualization of cardiac fibrosis by microcomputed tomography
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715218/
https://www.ncbi.nlm.nih.gov/pubmed/35005637
http://dx.doi.org/10.1016/j.xpro.2021.101055
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