Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice

Zika virus (ZIKV) is linked to congenital defects including microcephaly. An infection model that can recapitulate most microcephaly-related phenotypes is crucial for understanding ZIKV pathogenesis. Here, we present a protocol to generate ZIKV from an infectious clone through a reverse genetic syst...

Descripción completa

Detalles Bibliográficos
Autores principales: Dong, Shupeng, Xie, Xiaochun, Chen, Yujie, Long, Gang, Liang, Qiming, Zhao, Zhen, Zeng, Jianxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715324/
https://www.ncbi.nlm.nih.gov/pubmed/35005639
http://dx.doi.org/10.1016/j.xpro.2021.101057
_version_ 1784624106212163584
author Dong, Shupeng
Xie, Xiaochun
Chen, Yujie
Long, Gang
Liang, Qiming
Zhao, Zhen
Zeng, Jianxiong
author_facet Dong, Shupeng
Xie, Xiaochun
Chen, Yujie
Long, Gang
Liang, Qiming
Zhao, Zhen
Zeng, Jianxiong
author_sort Dong, Shupeng
collection PubMed
description Zika virus (ZIKV) is linked to congenital defects including microcephaly. An infection model that can recapitulate most microcephaly-related phenotypes is crucial for understanding ZIKV pathogenesis. Here, we present a protocol to generate ZIKV from an infectious clone through a reverse genetic system and subsequently perform embryonic brain infection with the rescued ZIKV in pregnant mice. We optimized several aspects of the procedures including virus rescue and in utero injection. This protocol facilitates reproducible investigation of virus-induced cortical development defects. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2020)
format Online
Article
Text
id pubmed-8715324
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-87153242022-01-06 Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice Dong, Shupeng Xie, Xiaochun Chen, Yujie Long, Gang Liang, Qiming Zhao, Zhen Zeng, Jianxiong STAR Protoc Protocol Zika virus (ZIKV) is linked to congenital defects including microcephaly. An infection model that can recapitulate most microcephaly-related phenotypes is crucial for understanding ZIKV pathogenesis. Here, we present a protocol to generate ZIKV from an infectious clone through a reverse genetic system and subsequently perform embryonic brain infection with the rescued ZIKV in pregnant mice. We optimized several aspects of the procedures including virus rescue and in utero injection. This protocol facilitates reproducible investigation of virus-induced cortical development defects. For complete details on the use and execution of this protocol, please refer to Zeng et al. (2020) Elsevier 2021-12-22 /pmc/articles/PMC8715324/ /pubmed/35005639 http://dx.doi.org/10.1016/j.xpro.2021.101057 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Dong, Shupeng
Xie, Xiaochun
Chen, Yujie
Long, Gang
Liang, Qiming
Zhao, Zhen
Zeng, Jianxiong
Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice
title Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice
title_full Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice
title_fullStr Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice
title_full_unstemmed Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice
title_short Protocol for in utero injection to investigate Zika virus-related fetal microcephaly in mice
title_sort protocol for in utero injection to investigate zika virus-related fetal microcephaly in mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715324/
https://www.ncbi.nlm.nih.gov/pubmed/35005639
http://dx.doi.org/10.1016/j.xpro.2021.101057
work_keys_str_mv AT dongshupeng protocolforinuteroinjectiontoinvestigatezikavirusrelatedfetalmicrocephalyinmice
AT xiexiaochun protocolforinuteroinjectiontoinvestigatezikavirusrelatedfetalmicrocephalyinmice
AT chenyujie protocolforinuteroinjectiontoinvestigatezikavirusrelatedfetalmicrocephalyinmice
AT longgang protocolforinuteroinjectiontoinvestigatezikavirusrelatedfetalmicrocephalyinmice
AT liangqiming protocolforinuteroinjectiontoinvestigatezikavirusrelatedfetalmicrocephalyinmice
AT zhaozhen protocolforinuteroinjectiontoinvestigatezikavirusrelatedfetalmicrocephalyinmice
AT zengjianxiong protocolforinuteroinjectiontoinvestigatezikavirusrelatedfetalmicrocephalyinmice

Ejemplares similares