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Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail

Consumption of prawns as a protein source has been on the rise worldwide with seafood identified as the predominant attributable source of human vibriosis. However, surveillance of non-cholera Vibrio is limited both in public health and in food. Using a population- and market share-weighted study de...

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Autores principales: Janecko, Nicol, Bloomfield, Samuel J., Palau, Raphaëlle, Mather, Alison E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715430/
https://www.ncbi.nlm.nih.gov/pubmed/34586050
http://dx.doi.org/10.1099/mgen.0.000647
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author Janecko, Nicol
Bloomfield, Samuel J.
Palau, Raphaëlle
Mather, Alison E.
author_facet Janecko, Nicol
Bloomfield, Samuel J.
Palau, Raphaëlle
Mather, Alison E.
author_sort Janecko, Nicol
collection PubMed
description Consumption of prawns as a protein source has been on the rise worldwide with seafood identified as the predominant attributable source of human vibriosis. However, surveillance of non-cholera Vibrio is limited both in public health and in food. Using a population- and market share-weighted study design, 211 prawn samples were collected and cultured for Vibrio spp. Contamination was detected in 46 % of samples, and multiple diverse Vibrio isolates were obtained from 34 % of positive samples. Whole genome sequencing (WGS) and phylogenetic analysis illustrated a comprehensive view of Vibrio species diversity in prawns available at retail, with no known pathogenicity markers identified in Vibrio parahaemolyticus and V. cholerae . Antimicrobial resistance genes were found in 77 % of isolates, and 12 % carried genes conferring resistance to three or more drug classes. Resistance genes were found predominantly in V. parahaemolyticus , though multiple resistance genes were also identified in V. cholerae and V. vulnificus . This study highlights the large diversity in Vibrio derived from prawns at retail, even within a single sample. Although there was little evidence in this study that prawns are a major source of vibriosis in the UK, surveillance of non-cholera Vibrio is very limited. This study illustrates the value of expanding WGS surveillance efforts of non-cholera Vibrios in the food chain to identify critical control points for food safety through the production system and to determine the full extent of the public health impact.
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spelling pubmed-87154302021-12-29 Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail Janecko, Nicol Bloomfield, Samuel J. Palau, Raphaëlle Mather, Alison E. Microb Genom Research Articles Consumption of prawns as a protein source has been on the rise worldwide with seafood identified as the predominant attributable source of human vibriosis. However, surveillance of non-cholera Vibrio is limited both in public health and in food. Using a population- and market share-weighted study design, 211 prawn samples were collected and cultured for Vibrio spp. Contamination was detected in 46 % of samples, and multiple diverse Vibrio isolates were obtained from 34 % of positive samples. Whole genome sequencing (WGS) and phylogenetic analysis illustrated a comprehensive view of Vibrio species diversity in prawns available at retail, with no known pathogenicity markers identified in Vibrio parahaemolyticus and V. cholerae . Antimicrobial resistance genes were found in 77 % of isolates, and 12 % carried genes conferring resistance to three or more drug classes. Resistance genes were found predominantly in V. parahaemolyticus , though multiple resistance genes were also identified in V. cholerae and V. vulnificus . This study highlights the large diversity in Vibrio derived from prawns at retail, even within a single sample. Although there was little evidence in this study that prawns are a major source of vibriosis in the UK, surveillance of non-cholera Vibrio is very limited. This study illustrates the value of expanding WGS surveillance efforts of non-cholera Vibrios in the food chain to identify critical control points for food safety through the production system and to determine the full extent of the public health impact. Microbiology Society 2021-09-28 /pmc/articles/PMC8715430/ /pubmed/34586050 http://dx.doi.org/10.1099/mgen.0.000647 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Articles
Janecko, Nicol
Bloomfield, Samuel J.
Palau, Raphaëlle
Mather, Alison E.
Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail
title Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail
title_full Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail
title_fullStr Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail
title_full_unstemmed Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail
title_short Whole genome sequencing reveals great diversity of Vibrio spp in prawns at retail
title_sort whole genome sequencing reveals great diversity of vibrio spp in prawns at retail
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8715430/
https://www.ncbi.nlm.nih.gov/pubmed/34586050
http://dx.doi.org/10.1099/mgen.0.000647
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