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ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma
ETS transcription factor ELK3 (ELK3), a novel oncogene, affects pathological processes and progression of many cancers in human tissues. However, it remains unclear whether ELK3, as a key gene, affects the pathological process of gliomas and the prognosis of patients with gliomas. This study aimed t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716454/ https://www.ncbi.nlm.nih.gov/pubmed/34976781 http://dx.doi.org/10.3389/fonc.2021.608748 |
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author | Liu, Zhendong Ren, Zhishuai Zhang, Cheng Qian, Rongjun Wang, Hongbo Wang, Jialin Zhang, Wang Liu, Binfeng Lian, Xiaoyu Wang, Yanbiao Guo, Yuqi Gao, Yanzheng |
author_facet | Liu, Zhendong Ren, Zhishuai Zhang, Cheng Qian, Rongjun Wang, Hongbo Wang, Jialin Zhang, Wang Liu, Binfeng Lian, Xiaoyu Wang, Yanbiao Guo, Yuqi Gao, Yanzheng |
author_sort | Liu, Zhendong |
collection | PubMed |
description | ETS transcription factor ELK3 (ELK3), a novel oncogene, affects pathological processes and progression of many cancers in human tissues. However, it remains unclear whether ELK3, as a key gene, affects the pathological process of gliomas and the prognosis of patients with gliomas. This study aimed to comprehensively and systematically reveal the correlation between ELK3 and the malignant progression of gliomas by analyzing clinical sample information stored in multiple databases. We revealed the putative mechanism of ELK3 involvement in malignant gliomas progression and identified a new and efficient biomarker for glioma diagnosis and targeted therapy. Based on the sample data from multiple databases and real-time quantitative polymerase chain reaction (RT-qPCR), the abnormally high expression of ELK3 in gliomas was confirmed. Kaplan-Meier and Cox regression analyses demonstrated that a high ELK3 expression was markedly associated with low patient survival and served as an independent biomarker of gliomas. Wilcox and Kruskal-Wallis tests revealed that expression of ELK3 was positively correlated with several clinical characteristics of patients with gliomas, such as age, WHO classification, and recurrence. Moreover, Cell Counting Kit‐8 (CCK-8), immunofluorescence, and wound healing assays confirmed that ELK3 overexpression markedly promoted the proliferation and migration of glioma cells. Finally, gene set enrichment analysis (GSEA) and western blotting confirmed that overexpression of ELK3 regulated the JAK–STAT signaling pathway and upregulate the expression of signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 (P-STAT3) to promote the malignant transition of gliomas. Therefore, ELK3 may serve as an efficient biomarker for the diagnosis and prognosis of gliomas and it can also be used as a therapeutic target to improve the poor prognosis of patients with gliomas. |
format | Online Article Text |
id | pubmed-8716454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87164542021-12-31 ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma Liu, Zhendong Ren, Zhishuai Zhang, Cheng Qian, Rongjun Wang, Hongbo Wang, Jialin Zhang, Wang Liu, Binfeng Lian, Xiaoyu Wang, Yanbiao Guo, Yuqi Gao, Yanzheng Front Oncol Oncology ETS transcription factor ELK3 (ELK3), a novel oncogene, affects pathological processes and progression of many cancers in human tissues. However, it remains unclear whether ELK3, as a key gene, affects the pathological process of gliomas and the prognosis of patients with gliomas. This study aimed to comprehensively and systematically reveal the correlation between ELK3 and the malignant progression of gliomas by analyzing clinical sample information stored in multiple databases. We revealed the putative mechanism of ELK3 involvement in malignant gliomas progression and identified a new and efficient biomarker for glioma diagnosis and targeted therapy. Based on the sample data from multiple databases and real-time quantitative polymerase chain reaction (RT-qPCR), the abnormally high expression of ELK3 in gliomas was confirmed. Kaplan-Meier and Cox regression analyses demonstrated that a high ELK3 expression was markedly associated with low patient survival and served as an independent biomarker of gliomas. Wilcox and Kruskal-Wallis tests revealed that expression of ELK3 was positively correlated with several clinical characteristics of patients with gliomas, such as age, WHO classification, and recurrence. Moreover, Cell Counting Kit‐8 (CCK-8), immunofluorescence, and wound healing assays confirmed that ELK3 overexpression markedly promoted the proliferation and migration of glioma cells. Finally, gene set enrichment analysis (GSEA) and western blotting confirmed that overexpression of ELK3 regulated the JAK–STAT signaling pathway and upregulate the expression of signal transducer and activator of transcription 3 (STAT3) and phosphorylated STAT3 (P-STAT3) to promote the malignant transition of gliomas. Therefore, ELK3 may serve as an efficient biomarker for the diagnosis and prognosis of gliomas and it can also be used as a therapeutic target to improve the poor prognosis of patients with gliomas. Frontiers Media S.A. 2021-12-16 /pmc/articles/PMC8716454/ /pubmed/34976781 http://dx.doi.org/10.3389/fonc.2021.608748 Text en Copyright © 2021 Liu, Ren, Zhang, Qian, Wang, Wang, Zhang, Liu, Lian, Wang, Guo and Gao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Liu, Zhendong Ren, Zhishuai Zhang, Cheng Qian, Rongjun Wang, Hongbo Wang, Jialin Zhang, Wang Liu, Binfeng Lian, Xiaoyu Wang, Yanbiao Guo, Yuqi Gao, Yanzheng ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma |
title |
ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma |
title_full |
ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma |
title_fullStr |
ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma |
title_full_unstemmed |
ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma |
title_short |
ELK3: A New Molecular Marker for the Diagnosis and Prognosis of Glioma |
title_sort | elk3: a new molecular marker for the diagnosis and prognosis of glioma |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716454/ https://www.ncbi.nlm.nih.gov/pubmed/34976781 http://dx.doi.org/10.3389/fonc.2021.608748 |
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