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Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways
Intestinal inflammation is a major threat to the health and growth of young animals such as piglets. As a next-generation probiotics, limited studies have shown that Akkermansia muciniphila could alleviate inflammation of intestinal epithelial cells (IECs). In this study, a TNF-α-induced inflammator...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716699/ https://www.ncbi.nlm.nih.gov/pubmed/34975882 http://dx.doi.org/10.3389/fimmu.2021.788638 |
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author | Luo, Yuheng Lan, Cong Xie, Kunhong Li, Hua Devillard, Estelle He, Jun Liu, Li Cai, Jingyi Tian, Gang Wu, Aimin Ren, Zhihua Chen, Daiwen Yu, Bing Huang, Zhiqing Zheng, Ping Mao, Xiangbing Yu, Jie Luo, Junqiu Yan, Hui Wang, Quyuan Wang, Huifen Tang, Jiayong |
author_facet | Luo, Yuheng Lan, Cong Xie, Kunhong Li, Hua Devillard, Estelle He, Jun Liu, Li Cai, Jingyi Tian, Gang Wu, Aimin Ren, Zhihua Chen, Daiwen Yu, Bing Huang, Zhiqing Zheng, Ping Mao, Xiangbing Yu, Jie Luo, Junqiu Yan, Hui Wang, Quyuan Wang, Huifen Tang, Jiayong |
author_sort | Luo, Yuheng |
collection | PubMed |
description | Intestinal inflammation is a major threat to the health and growth of young animals such as piglets. As a next-generation probiotics, limited studies have shown that Akkermansia muciniphila could alleviate inflammation of intestinal epithelial cells (IECs). In this study, a TNF-α-induced inflammatory model of IPEC-J2 cells, the intestinal porcine enterocytes, was built to evaluate the effects of active or inactive A. muciniphila on the inflammation of IECs. The viability of IPEC-J2 cells was the highest when treated with active (10(8) copies/mL) or inactive (10(9) copies/mL) A. muciniphila for 7.5 h (P < 0.01). Treated with 20 ng/mL of TNF-α and followed by a treatment of A. muciniphila, the mRNA level of proinflammatory cytokines (IL-8, IL-1β, IL-6 and TNF-α) was remarkably reduced (P < 0.05) along with the increased mRNA level of tight junction proteins (ZO-1 and Occludin, P < 0.05). Flow cytometry analysis showed that active or inactive A. muciniphila significantly suppressed the rate of the early and total apoptotic of the inflammatory IPEC-J2 cells (P < 0.05). According to results of transcriptome sequencing, active and inactive A. muciniphila may decline cell apoptosis by down-regulating the expression of key genes in calcium signaling pathway, or up-regulating the expression of key genes in cell cycle signaling pathway. And the bacterium may alleviate the inflammation of IECs by down-regulating the expression of PI3K upstream receptor genes. Our results indicate that A. muciniphila may be a promising NGP targeting intestinal inflammation. |
format | Online Article Text |
id | pubmed-8716699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87166992021-12-31 Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways Luo, Yuheng Lan, Cong Xie, Kunhong Li, Hua Devillard, Estelle He, Jun Liu, Li Cai, Jingyi Tian, Gang Wu, Aimin Ren, Zhihua Chen, Daiwen Yu, Bing Huang, Zhiqing Zheng, Ping Mao, Xiangbing Yu, Jie Luo, Junqiu Yan, Hui Wang, Quyuan Wang, Huifen Tang, Jiayong Front Immunol Immunology Intestinal inflammation is a major threat to the health and growth of young animals such as piglets. As a next-generation probiotics, limited studies have shown that Akkermansia muciniphila could alleviate inflammation of intestinal epithelial cells (IECs). In this study, a TNF-α-induced inflammatory model of IPEC-J2 cells, the intestinal porcine enterocytes, was built to evaluate the effects of active or inactive A. muciniphila on the inflammation of IECs. The viability of IPEC-J2 cells was the highest when treated with active (10(8) copies/mL) or inactive (10(9) copies/mL) A. muciniphila for 7.5 h (P < 0.01). Treated with 20 ng/mL of TNF-α and followed by a treatment of A. muciniphila, the mRNA level of proinflammatory cytokines (IL-8, IL-1β, IL-6 and TNF-α) was remarkably reduced (P < 0.05) along with the increased mRNA level of tight junction proteins (ZO-1 and Occludin, P < 0.05). Flow cytometry analysis showed that active or inactive A. muciniphila significantly suppressed the rate of the early and total apoptotic of the inflammatory IPEC-J2 cells (P < 0.05). According to results of transcriptome sequencing, active and inactive A. muciniphila may decline cell apoptosis by down-regulating the expression of key genes in calcium signaling pathway, or up-regulating the expression of key genes in cell cycle signaling pathway. And the bacterium may alleviate the inflammation of IECs by down-regulating the expression of PI3K upstream receptor genes. Our results indicate that A. muciniphila may be a promising NGP targeting intestinal inflammation. Frontiers Media S.A. 2021-12-16 /pmc/articles/PMC8716699/ /pubmed/34975882 http://dx.doi.org/10.3389/fimmu.2021.788638 Text en Copyright © 2021 Luo, Lan, Xie, Li, Devillard, He, Liu, Cai, Tian, Wu, Ren, Chen, Yu, Huang, Zheng, Mao, Yu, Luo, Yan, Wang, Wang and Tang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Luo, Yuheng Lan, Cong Xie, Kunhong Li, Hua Devillard, Estelle He, Jun Liu, Li Cai, Jingyi Tian, Gang Wu, Aimin Ren, Zhihua Chen, Daiwen Yu, Bing Huang, Zhiqing Zheng, Ping Mao, Xiangbing Yu, Jie Luo, Junqiu Yan, Hui Wang, Quyuan Wang, Huifen Tang, Jiayong Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways |
title | Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways |
title_full | Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways |
title_fullStr | Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways |
title_full_unstemmed | Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways |
title_short | Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways |
title_sort | active or autoclaved akkermansia muciniphila relieves tnf-α-induced inflammation in intestinal epithelial cells through distinct pathways |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716699/ https://www.ncbi.nlm.nih.gov/pubmed/34975882 http://dx.doi.org/10.3389/fimmu.2021.788638 |
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