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Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways

Intestinal inflammation is a major threat to the health and growth of young animals such as piglets. As a next-generation probiotics, limited studies have shown that Akkermansia muciniphila could alleviate inflammation of intestinal epithelial cells (IECs). In this study, a TNF-α-induced inflammator...

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Autores principales: Luo, Yuheng, Lan, Cong, Xie, Kunhong, Li, Hua, Devillard, Estelle, He, Jun, Liu, Li, Cai, Jingyi, Tian, Gang, Wu, Aimin, Ren, Zhihua, Chen, Daiwen, Yu, Bing, Huang, Zhiqing, Zheng, Ping, Mao, Xiangbing, Yu, Jie, Luo, Junqiu, Yan, Hui, Wang, Quyuan, Wang, Huifen, Tang, Jiayong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716699/
https://www.ncbi.nlm.nih.gov/pubmed/34975882
http://dx.doi.org/10.3389/fimmu.2021.788638
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author Luo, Yuheng
Lan, Cong
Xie, Kunhong
Li, Hua
Devillard, Estelle
He, Jun
Liu, Li
Cai, Jingyi
Tian, Gang
Wu, Aimin
Ren, Zhihua
Chen, Daiwen
Yu, Bing
Huang, Zhiqing
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
Wang, Quyuan
Wang, Huifen
Tang, Jiayong
author_facet Luo, Yuheng
Lan, Cong
Xie, Kunhong
Li, Hua
Devillard, Estelle
He, Jun
Liu, Li
Cai, Jingyi
Tian, Gang
Wu, Aimin
Ren, Zhihua
Chen, Daiwen
Yu, Bing
Huang, Zhiqing
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
Wang, Quyuan
Wang, Huifen
Tang, Jiayong
author_sort Luo, Yuheng
collection PubMed
description Intestinal inflammation is a major threat to the health and growth of young animals such as piglets. As a next-generation probiotics, limited studies have shown that Akkermansia muciniphila could alleviate inflammation of intestinal epithelial cells (IECs). In this study, a TNF-α-induced inflammatory model of IPEC-J2 cells, the intestinal porcine enterocytes, was built to evaluate the effects of active or inactive A. muciniphila on the inflammation of IECs. The viability of IPEC-J2 cells was the highest when treated with active (10(8) copies/mL) or inactive (10(9) copies/mL) A. muciniphila for 7.5 h (P < 0.01). Treated with 20 ng/mL of TNF-α and followed by a treatment of A. muciniphila, the mRNA level of proinflammatory cytokines (IL-8, IL-1β, IL-6 and TNF-α) was remarkably reduced (P < 0.05) along with the increased mRNA level of tight junction proteins (ZO-1 and Occludin, P < 0.05). Flow cytometry analysis showed that active or inactive A. muciniphila significantly suppressed the rate of the early and total apoptotic of the inflammatory IPEC-J2 cells (P < 0.05). According to results of transcriptome sequencing, active and inactive A. muciniphila may decline cell apoptosis by down-regulating the expression of key genes in calcium signaling pathway, or up-regulating the expression of key genes in cell cycle signaling pathway. And the bacterium may alleviate the inflammation of IECs by down-regulating the expression of PI3K upstream receptor genes. Our results indicate that A. muciniphila may be a promising NGP targeting intestinal inflammation.
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spelling pubmed-87166992021-12-31 Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways Luo, Yuheng Lan, Cong Xie, Kunhong Li, Hua Devillard, Estelle He, Jun Liu, Li Cai, Jingyi Tian, Gang Wu, Aimin Ren, Zhihua Chen, Daiwen Yu, Bing Huang, Zhiqing Zheng, Ping Mao, Xiangbing Yu, Jie Luo, Junqiu Yan, Hui Wang, Quyuan Wang, Huifen Tang, Jiayong Front Immunol Immunology Intestinal inflammation is a major threat to the health and growth of young animals such as piglets. As a next-generation probiotics, limited studies have shown that Akkermansia muciniphila could alleviate inflammation of intestinal epithelial cells (IECs). In this study, a TNF-α-induced inflammatory model of IPEC-J2 cells, the intestinal porcine enterocytes, was built to evaluate the effects of active or inactive A. muciniphila on the inflammation of IECs. The viability of IPEC-J2 cells was the highest when treated with active (10(8) copies/mL) or inactive (10(9) copies/mL) A. muciniphila for 7.5 h (P < 0.01). Treated with 20 ng/mL of TNF-α and followed by a treatment of A. muciniphila, the mRNA level of proinflammatory cytokines (IL-8, IL-1β, IL-6 and TNF-α) was remarkably reduced (P < 0.05) along with the increased mRNA level of tight junction proteins (ZO-1 and Occludin, P < 0.05). Flow cytometry analysis showed that active or inactive A. muciniphila significantly suppressed the rate of the early and total apoptotic of the inflammatory IPEC-J2 cells (P < 0.05). According to results of transcriptome sequencing, active and inactive A. muciniphila may decline cell apoptosis by down-regulating the expression of key genes in calcium signaling pathway, or up-regulating the expression of key genes in cell cycle signaling pathway. And the bacterium may alleviate the inflammation of IECs by down-regulating the expression of PI3K upstream receptor genes. Our results indicate that A. muciniphila may be a promising NGP targeting intestinal inflammation. Frontiers Media S.A. 2021-12-16 /pmc/articles/PMC8716699/ /pubmed/34975882 http://dx.doi.org/10.3389/fimmu.2021.788638 Text en Copyright © 2021 Luo, Lan, Xie, Li, Devillard, He, Liu, Cai, Tian, Wu, Ren, Chen, Yu, Huang, Zheng, Mao, Yu, Luo, Yan, Wang, Wang and Tang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Luo, Yuheng
Lan, Cong
Xie, Kunhong
Li, Hua
Devillard, Estelle
He, Jun
Liu, Li
Cai, Jingyi
Tian, Gang
Wu, Aimin
Ren, Zhihua
Chen, Daiwen
Yu, Bing
Huang, Zhiqing
Zheng, Ping
Mao, Xiangbing
Yu, Jie
Luo, Junqiu
Yan, Hui
Wang, Quyuan
Wang, Huifen
Tang, Jiayong
Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways
title Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways
title_full Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways
title_fullStr Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways
title_full_unstemmed Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways
title_short Active or Autoclaved Akkermansia muciniphila Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways
title_sort active or autoclaved akkermansia muciniphila relieves tnf-α-induced inflammation in intestinal epithelial cells through distinct pathways
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716699/
https://www.ncbi.nlm.nih.gov/pubmed/34975882
http://dx.doi.org/10.3389/fimmu.2021.788638
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