Cargando…

Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study

INTRODUCTION: Among metabolic disorders, gestational diabetes mellitus (GDM) is specified as hyperglycemia caused by glucose or carbohydrate intolerance defects. GDM is distinguished by oxidative stress, and has been connected to mitochondrial dysfunction. Previous studies have documented the relati...

Descripción completa

Detalles Bibliográficos
Autores principales: Alharbi, Khalid Khalaf, Alsaikhan, Abdullah Sulaiman, Alshammary, Amal F., Al-Hakeem, Malak Mohammed, Ali Khan, Imran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716902/
https://www.ncbi.nlm.nih.gov/pubmed/35002430
http://dx.doi.org/10.1016/j.sjbs.2021.08.102
_version_ 1784624418789523456
author Alharbi, Khalid Khalaf
Alsaikhan, Abdullah Sulaiman
Alshammary, Amal F.
Al-Hakeem, Malak Mohammed
Ali Khan, Imran
author_facet Alharbi, Khalid Khalaf
Alsaikhan, Abdullah Sulaiman
Alshammary, Amal F.
Al-Hakeem, Malak Mohammed
Ali Khan, Imran
author_sort Alharbi, Khalid Khalaf
collection PubMed
description INTRODUCTION: Among metabolic disorders, gestational diabetes mellitus (GDM) is specified as hyperglycemia caused by glucose or carbohydrate intolerance defects. GDM is distinguished by oxidative stress, and has been connected to mitochondrial dysfunction. Previous studies have documented the relation between A12026G, A8344G and A3243G mutations in ND4, tRNA(Leu(UUR)), and tRNA(Lys) genes in different modes of diabetes. AIM: The purpose of this study was to investigate into the relationship between GDM women and common mitochondrial mutations including A12026, A8344G, and A3243G in Saudi women. METHODS: In this case-control study, we have opted 96 GDM and 102 non-GDM pregnant women and DNA was extracted using EDTA blood and based on specific primers, Polymerase Chain Reaction was followed and then Restriction Fragment Length Polymorphism (RFLP) analysis was performed. Restriction enzymes was cross-checked with Lambda DNA and 10% of the purified PCR products were performed the Sanger sequencing analysis to reconfirm the RFLP analysis of the studied results. RESULTS: None of the heterozygous and homozygous mutations were not observed in our study. All the subjects were turned to be homozygous normal genotypes. CONCLUSION: This study confirms that A12026, A8344G, and A3243G mutations have no role in the Saudi women with GDM.
format Online
Article
Text
id pubmed-8716902
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-87169022022-01-06 Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study Alharbi, Khalid Khalaf Alsaikhan, Abdullah Sulaiman Alshammary, Amal F. Al-Hakeem, Malak Mohammed Ali Khan, Imran Saudi J Biol Sci Original Article INTRODUCTION: Among metabolic disorders, gestational diabetes mellitus (GDM) is specified as hyperglycemia caused by glucose or carbohydrate intolerance defects. GDM is distinguished by oxidative stress, and has been connected to mitochondrial dysfunction. Previous studies have documented the relation between A12026G, A8344G and A3243G mutations in ND4, tRNA(Leu(UUR)), and tRNA(Lys) genes in different modes of diabetes. AIM: The purpose of this study was to investigate into the relationship between GDM women and common mitochondrial mutations including A12026, A8344G, and A3243G in Saudi women. METHODS: In this case-control study, we have opted 96 GDM and 102 non-GDM pregnant women and DNA was extracted using EDTA blood and based on specific primers, Polymerase Chain Reaction was followed and then Restriction Fragment Length Polymorphism (RFLP) analysis was performed. Restriction enzymes was cross-checked with Lambda DNA and 10% of the purified PCR products were performed the Sanger sequencing analysis to reconfirm the RFLP analysis of the studied results. RESULTS: None of the heterozygous and homozygous mutations were not observed in our study. All the subjects were turned to be homozygous normal genotypes. CONCLUSION: This study confirms that A12026, A8344G, and A3243G mutations have no role in the Saudi women with GDM. Elsevier 2022-01 2021-09-06 /pmc/articles/PMC8716902/ /pubmed/35002430 http://dx.doi.org/10.1016/j.sjbs.2021.08.102 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Alharbi, Khalid Khalaf
Alsaikhan, Abdullah Sulaiman
Alshammary, Amal F.
Al-Hakeem, Malak Mohammed
Ali Khan, Imran
Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study
title Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study
title_full Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study
title_fullStr Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study
title_full_unstemmed Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study
title_short Screening of mitochondrial mutations in Saudi women diagnosed with gestational diabetes mellitus: A non-replicative case-control study
title_sort screening of mitochondrial mutations in saudi women diagnosed with gestational diabetes mellitus: a non-replicative case-control study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716902/
https://www.ncbi.nlm.nih.gov/pubmed/35002430
http://dx.doi.org/10.1016/j.sjbs.2021.08.102
work_keys_str_mv AT alharbikhalidkhalaf screeningofmitochondrialmutationsinsaudiwomendiagnosedwithgestationaldiabetesmellitusanonreplicativecasecontrolstudy
AT alsaikhanabdullahsulaiman screeningofmitochondrialmutationsinsaudiwomendiagnosedwithgestationaldiabetesmellitusanonreplicativecasecontrolstudy
AT alshammaryamalf screeningofmitochondrialmutationsinsaudiwomendiagnosedwithgestationaldiabetesmellitusanonreplicativecasecontrolstudy
AT alhakeemmalakmohammed screeningofmitochondrialmutationsinsaudiwomendiagnosedwithgestationaldiabetesmellitusanonreplicativecasecontrolstudy
AT alikhanimran screeningofmitochondrialmutationsinsaudiwomendiagnosedwithgestationaldiabetesmellitusanonreplicativecasecontrolstudy