Cargando…

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

ETHNOPHARMACOLOGICAL RELEVANCE: Since ancient times, herbal medicines have been applied in the treatment of cancer. Tea, derivative from the dried leaves of Camellia sinensis (L.) Kuntze plant is the most popular beverage globally after water and is available in various forms. Green tea has been exp...

Descripción completa

Detalles Bibliográficos
Autores principales: Hamad Shareef, Suhayla, Abdel Aziz Ibrahim, Ibrahim, Alzahrani, Abdullah R., Al-Medhtiy, Morteta H., Ameen Abdulla, Mahmood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716963/
https://www.ncbi.nlm.nih.gov/pubmed/35002452
http://dx.doi.org/10.1016/j.sjbs.2021.09.023
_version_ 1784624433379409920
author Hamad Shareef, Suhayla
Abdel Aziz Ibrahim, Ibrahim
Alzahrani, Abdullah R.
Al-Medhtiy, Morteta H.
Ameen Abdulla, Mahmood
author_facet Hamad Shareef, Suhayla
Abdel Aziz Ibrahim, Ibrahim
Alzahrani, Abdullah R.
Al-Medhtiy, Morteta H.
Ameen Abdulla, Mahmood
author_sort Hamad Shareef, Suhayla
collection PubMed
description ETHNOPHARMACOLOGICAL RELEVANCE: Since ancient times, herbal medicines have been applied in the treatment of cancer. Tea, derivative from the dried leaves of Camellia sinensis (L.) Kuntze plant is the most popular beverage globally after water and is available in various forms. Green tea has been expansively investigated for its beneficial properties of cancer prevention and therapy. The goal of the research: The current study was conducted to evaluate the hepaprotective character of methanolic green tea extract and its mechanism of action contrary to thioacetamide (TAA)-produced liver fibrosis of Sprague Dawley rats. MATERIALS AND METHODS: Thirty rodents were equally placed in 5 clusters including normal control, TAA group as a positive control, silymarin as standard drug control, and treatment groups consisting of high dose and a low dose Camellia sinensis. Rats in experimental clusters by mouth fed with C. sinensis at 250 mg/kg or 500 mg/kg daily for 2 months. After 60 days, all rats were sacrificed. Blood specimens were gathered for liver biochemical examination. Livers of all groups were dissected out and subjected to histopathological examination through the Hematoxylin and Eosin stain, Masson trichrome, and immunohistochemistry stains (PCNA). Liver tissue homogenate was also analyzed for antioxidant activity parameters. RESULTS: Gross morphological examination showed a regular liver architecture in C. sinensis fed collections compared to the TAA sets. Histology of rat’s liver fed with C. sinensis showed an important decrease in the liver index with hepatic cells propagation, mild cellular injury, and immunostaining showed significant down-expression of proliferating cell nuclear antigen (PCNA). TAA produced liver fibrosis through a significant increase in serum alanine transferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin. Total protein and albumin also decreased in the TAA group. Moreover, the reduction of antioxidant enzyme activity including superoxide dismutase and catalase as well as the increase in malondialdehyde was detected in the TAA control group. Meanwhile, an abnormal level of liver biochemical parameters was restored closer to the normal levels in serum of the C. sinensis-fed clusters. In addition, C. sinensis fed assemblies showed elevated antioxidative enzymes activity with a reduction in malondialdehyde level comparable to the levels in silymarin-treated rats. CONCLUSIONS: Green tea potentially inhibited the progression of liver cirrhosis, down -regulation of PCNA proliferation, prevented oxidation of hepatocytes, recovered SOD and CAT enzymes, condensed MDA and reduced cellular inflammation.
format Online
Article
Text
id pubmed-8716963
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-87169632022-01-06 Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats Hamad Shareef, Suhayla Abdel Aziz Ibrahim, Ibrahim Alzahrani, Abdullah R. Al-Medhtiy, Morteta H. Ameen Abdulla, Mahmood Saudi J Biol Sci Original Article ETHNOPHARMACOLOGICAL RELEVANCE: Since ancient times, herbal medicines have been applied in the treatment of cancer. Tea, derivative from the dried leaves of Camellia sinensis (L.) Kuntze plant is the most popular beverage globally after water and is available in various forms. Green tea has been expansively investigated for its beneficial properties of cancer prevention and therapy. The goal of the research: The current study was conducted to evaluate the hepaprotective character of methanolic green tea extract and its mechanism of action contrary to thioacetamide (TAA)-produced liver fibrosis of Sprague Dawley rats. MATERIALS AND METHODS: Thirty rodents were equally placed in 5 clusters including normal control, TAA group as a positive control, silymarin as standard drug control, and treatment groups consisting of high dose and a low dose Camellia sinensis. Rats in experimental clusters by mouth fed with C. sinensis at 250 mg/kg or 500 mg/kg daily for 2 months. After 60 days, all rats were sacrificed. Blood specimens were gathered for liver biochemical examination. Livers of all groups were dissected out and subjected to histopathological examination through the Hematoxylin and Eosin stain, Masson trichrome, and immunohistochemistry stains (PCNA). Liver tissue homogenate was also analyzed for antioxidant activity parameters. RESULTS: Gross morphological examination showed a regular liver architecture in C. sinensis fed collections compared to the TAA sets. Histology of rat’s liver fed with C. sinensis showed an important decrease in the liver index with hepatic cells propagation, mild cellular injury, and immunostaining showed significant down-expression of proliferating cell nuclear antigen (PCNA). TAA produced liver fibrosis through a significant increase in serum alanine transferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin. Total protein and albumin also decreased in the TAA group. Moreover, the reduction of antioxidant enzyme activity including superoxide dismutase and catalase as well as the increase in malondialdehyde was detected in the TAA control group. Meanwhile, an abnormal level of liver biochemical parameters was restored closer to the normal levels in serum of the C. sinensis-fed clusters. In addition, C. sinensis fed assemblies showed elevated antioxidative enzymes activity with a reduction in malondialdehyde level comparable to the levels in silymarin-treated rats. CONCLUSIONS: Green tea potentially inhibited the progression of liver cirrhosis, down -regulation of PCNA proliferation, prevented oxidation of hepatocytes, recovered SOD and CAT enzymes, condensed MDA and reduced cellular inflammation. Elsevier 2022-01 2021-09-16 /pmc/articles/PMC8716963/ /pubmed/35002452 http://dx.doi.org/10.1016/j.sjbs.2021.09.023 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Hamad Shareef, Suhayla
Abdel Aziz Ibrahim, Ibrahim
Alzahrani, Abdullah R.
Al-Medhtiy, Morteta H.
Ameen Abdulla, Mahmood
Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats
title Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats
title_full Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats
title_fullStr Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats
title_full_unstemmed Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats
title_short Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats
title_sort hepatoprotective effects of methanolic extract of green tea against thioacetamide-induced liver injury in sprague dawley rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8716963/
https://www.ncbi.nlm.nih.gov/pubmed/35002452
http://dx.doi.org/10.1016/j.sjbs.2021.09.023
work_keys_str_mv AT hamadshareefsuhayla hepatoprotectiveeffectsofmethanolicextractofgreenteaagainstthioacetamideinducedliverinjuryinspraguedawleyrats
AT abdelazizibrahimibrahim hepatoprotectiveeffectsofmethanolicextractofgreenteaagainstthioacetamideinducedliverinjuryinspraguedawleyrats
AT alzahraniabdullahr hepatoprotectiveeffectsofmethanolicextractofgreenteaagainstthioacetamideinducedliverinjuryinspraguedawleyrats
AT almedhtiymortetah hepatoprotectiveeffectsofmethanolicextractofgreenteaagainstthioacetamideinducedliverinjuryinspraguedawleyrats
AT ameenabdullamahmood hepatoprotectiveeffectsofmethanolicextractofgreenteaagainstthioacetamideinducedliverinjuryinspraguedawleyrats