Cargando…

Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

The early diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is required to identify and isolate contagious patients to prevent further transmission of SARS-CoV-2. In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for...

Descripción completa

Detalles Bibliográficos
Autores principales:	Durand, Mathieu, Thibault, Philippe, Lévesque, Simon, Brault, Ariane, Carignan, Alex, Valiquette, Louis, Martin, Philippe, Labbé, Simon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Shared Science Publishers OG 2021
Materias:	Research Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717086/ https://www.ncbi.nlm.nih.gov/pubmed/35083313 http://dx.doi.org/10.15698/mic2022.01.767

Ejemplares similares

Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR
por: Levesque-Sergerie, Jean-Philippe, et al.
Publicado: (2007)

Cuf2 Is a Transcriptional Co-Regulator that Interacts with Mei4 for Timely Expression of Middle-Phase Meiotic Genes
por: Ioannoni, Raphaël, et al.
Publicado: (2016)

Sib1, Sib2, and Sib3 proteins are required for ferrichrome-mediated cross-feeding interaction between Schizosaccharomyces pombe and Saccharomyces cerevisiae
por: Brault, Ariane, et al.
Publicado: (2022)

Does BCG provide long-term protection against SARS-CoV-2 infection? A case–control study in Quebec, Canada
por: Pépin, Jacques, et al.
Publicado: (2021)

Fra2 Is a Co-Regulator of Fep1 Inhibition in Response to Iron Starvation
por: Jacques, Jean-François, et al.
Publicado: (2014)

Updated and Validated Pan-Coronavirus PCR Assay to Detect All Coronavirus Genera
por: Holbrook, Myndi G., et al.
Publicado: (2021)

An Offer You Can’t Refuse: Clinical Impact of Accepting or Rejecting a Recommendation from an Antibiotic Stewardship Program
por: Carignan, Alex, et al.
Publicado: (2017)

Clinical validation of an automated reverse transcription-insulated isothermal PCR assay for the detection of severe acute respiratory syndrome coronavirus 2
por: Chang, Pai-Ling, et al.
Publicado: (2021)

Development of two TaqMan real-time reverse transcription-PCR assays for the detection of severe acute respiratory syndrome coronavirus-2
por: Liu, Yiwei, et al.
Publicado: (2020)

Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant
por: Bedotto, Marielle, et al.
Publicado: (2021)

Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants
por: Bedotto, Marielle, et al.
Publicado: (2021)

Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells
por: Brault, Ariane, et al.
Publicado: (2016)

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
por: O'Sullivan, Matthew V. N., et al.
Publicado: (2011)

Printing 2-Dimentional Droplet Array for Single-Cell Reverse Transcription Quantitative PCR Assay with a Microfluidic Robot
por: Zhu, Ying, et al.
Publicado: (2015)

Secular trends in incidence of invasive beta-hemolytic streptococci and efficacy of adjunctive therapy in Quebec, Canada, 1996-2016
por: Couture-Cossette, Antoine, et al.
Publicado: (2018)

Selection of housekeeping genes for use in quantitative reverse transcription PCR assays on the murine cornea
por: Ren, Shengwei, et al.
Publicado: (2010)

Smaller reaction volume of triplex taqman real‐time reverse transcription‐PCR assays for diagnosing coronavirus disease 2019
por: Dong, Wenxue, et al.
Publicado: (2021)

Comparison of commercial realtime reverse transcription PCR assays for the detection of SARS-CoV-2
por: Iglói, Zsófia, et al.
Publicado: (2020)

Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome
por: Khan, Kashif Aziz, et al.
Publicado: (2020)

Is Antimicrobial Dosing Adjustment Associated with Better Outcomes in Patients with Severe Obesity and Bloodstream Infections? An Exploratory Study
por: Sirard, Stéphanie, et al.
Publicado: (2020)

Energy Conservation and the Promotion of Legionella pneumophila Growth: The Probable Role of Heat Exchangers in a Nosocomial Outbreak
por: Bédard, Emilie, et al.
Publicado: (2016)

Comparison of SARS-CoV-2 detection from combined nasopharyngeal/oropharyngeal swab samples by a laboratory-developed real-time RT-PCR test and the Roche SARS-CoV-2 assay on a cobas 8800 instrument
por: Boutin, Catherine-Audrey, et al.
Publicado: (2020)

PCR assay for the detection and the identification of atypical canine coronavirus in dogs
por: Pratelli, Annamaria, et al.
Publicado: (2002)

Development of a nested PCR assay for the detection of canine coronavirus
por: Pratelli, A, et al.
Publicado: (1999)

An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes
por: Sun, Junfeng, et al.
Publicado: (2015)

Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants
por: Gand, Mathieu, et al.
Publicado: (2021)

Quantitative reverse transcription PCR assay to detect a genetic marker of pyrethroid resistance in Culex mosquitoes
por: Hager, Kelli M., et al.
Publicado: (2022)

hnRNP A1/A2 and Sam68 collaborate with SRSF10 to control the alternative splicing response to oxaliplatin-mediated DNA damage
por: Cloutier, Alexandre, et al.
Publicado: (2018)

Multiplex PCR Assays for Identifying all Major Severe Acute Respiratory Syndrome Coronavirus 2 Variants
por: Dikdan, Ryan J., et al.
Publicado: (2022)

Comparison of Multiplex Real-Time PCR and PCR-Reverse Blot Hybridization Assays for the Direct and Rapid Detection of Porcine Circovirus Type 2 Genotypes
por: Wang, Hye-young, et al.
Publicado: (2020)

Development and validation of a new triplex real-time quantitative reverse Transcriptase-PCR assay for the clinical detection of SARS-CoV-2
por: Gadkar, Vijay J., et al.
Publicado: (2021)

A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
por: Vijgen, Leen, et al.
Publicado: (2007)

Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis
por: Soma, Takehisa
Publicado: (2015)

A RT-PCR assay for the detection of coronaviruses from four genera
por: Xiu, Leshan, et al.
Publicado: (2020)

Validation of saliva sampling as an alternative to oro-nasopharyngeal swab for detection of SARS-CoV-2 using unextracted rRT-PCR with the Allplex 2019-nCoV assay
por: Bergevin, Marco Andres, et al.
Publicado: (2021)

Evaluation of a quantitative RT-PCR assay for the detection of the emerging coronavirus SARS-CoV-2 using a high throughput system
por: Pfefferle, Susanne, et al.
Publicado: (2020)

Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays
por: Mikeska, Thomas, et al.
Publicado: (2009)

Development of a Neutralization Assay for Influenza Virus Using an Endpoint Assessment Based on Quantitative Reverse-Transcription PCR
por: Teferedegne, Belete, et al.
Publicado: (2013)

A quantitative reverse-transcriptase PCR assay for the assessment of drug activities against intracellular Theileria annulata schizonts
por: Hostettler, Isabel, et al.
Publicado: (2014)

Detection and differentiation of Schmallenberg, Akabane and Aino viruses by one-step multiplex reverse-transcriptase quantitative PCR assay
por: Lee, Ji-Hye, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_21dngl07mj4kpl8k7skuabdco2