Cargando…

The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)

The Ductus Arteriosus (DA) is a fetal vessel that connects the aorta to the pulmonary artery ensuring that placental oxygenated blood is diverted from the lungs to the systemic circulation. Following exposure to oxygen (O(2)), in the first few days of life, the DA responds with a functional closure...

Descripción completa

Detalles Bibliográficos
Autores principales: Bentley, Rachel E.T., Hindmarch, Charles C.T., Dunham-Snary, Kimberly J., Snetsinger, Brooke, Mewburn, Jeffrey D., Thébaud, Arthur, Lima, Patricia D.A., Thébaud, Bernard, Archer, Stephen L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717140/
https://www.ncbi.nlm.nih.gov/pubmed/35005134
http://dx.doi.org/10.1016/j.dib.2021.107736
_version_ 1784624473828229120
author Bentley, Rachel E.T.
Hindmarch, Charles C.T.
Dunham-Snary, Kimberly J.
Snetsinger, Brooke
Mewburn, Jeffrey D.
Thébaud, Arthur
Lima, Patricia D.A.
Thébaud, Bernard
Archer, Stephen L.
author_facet Bentley, Rachel E.T.
Hindmarch, Charles C.T.
Dunham-Snary, Kimberly J.
Snetsinger, Brooke
Mewburn, Jeffrey D.
Thébaud, Arthur
Lima, Patricia D.A.
Thébaud, Bernard
Archer, Stephen L.
author_sort Bentley, Rachel E.T.
collection PubMed
description The Ductus Arteriosus (DA) is a fetal vessel that connects the aorta to the pulmonary artery ensuring that placental oxygenated blood is diverted from the lungs to the systemic circulation. Following exposure to oxygen (O(2)), in the first few days of life, the DA responds with a functional closure that is followed by anatomical closure. Here, we study human DA smooth muscle cells (DASMC) taken from 10 term infants during congenital heart surgery. Purification of these cells using flow cytometry ensured a pure population of DASMCs, which we confirmed as responsive to O(2). An oxygen-induced increase in intracellular calcium of 18.1%±4.4% and SMC constriction (-27%±1.5% shortening) occurred in all cell lines within five minutes. These cells were maintained in either hypoxia (2.5% O(2)), mimicking in utero conditions or in normoxia (19% O(2)) mimicking neonate conditions. We then used 3’ RNAsequencing to identify the transcriptome of DASMCs in each condition [1]. In this paper, we present the full differentially regulated gene list from this experiment.
format Online
Article
Text
id pubmed-8717140
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-87171402022-01-06 The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC) Bentley, Rachel E.T. Hindmarch, Charles C.T. Dunham-Snary, Kimberly J. Snetsinger, Brooke Mewburn, Jeffrey D. Thébaud, Arthur Lima, Patricia D.A. Thébaud, Bernard Archer, Stephen L. Data Brief Data Article The Ductus Arteriosus (DA) is a fetal vessel that connects the aorta to the pulmonary artery ensuring that placental oxygenated blood is diverted from the lungs to the systemic circulation. Following exposure to oxygen (O(2)), in the first few days of life, the DA responds with a functional closure that is followed by anatomical closure. Here, we study human DA smooth muscle cells (DASMC) taken from 10 term infants during congenital heart surgery. Purification of these cells using flow cytometry ensured a pure population of DASMCs, which we confirmed as responsive to O(2). An oxygen-induced increase in intracellular calcium of 18.1%±4.4% and SMC constriction (-27%±1.5% shortening) occurred in all cell lines within five minutes. These cells were maintained in either hypoxia (2.5% O(2)), mimicking in utero conditions or in normoxia (19% O(2)) mimicking neonate conditions. We then used 3’ RNAsequencing to identify the transcriptome of DASMCs in each condition [1]. In this paper, we present the full differentially regulated gene list from this experiment. Elsevier 2021-12-20 /pmc/articles/PMC8717140/ /pubmed/35005134 http://dx.doi.org/10.1016/j.dib.2021.107736 Text en © 2021 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data Article
Bentley, Rachel E.T.
Hindmarch, Charles C.T.
Dunham-Snary, Kimberly J.
Snetsinger, Brooke
Mewburn, Jeffrey D.
Thébaud, Arthur
Lima, Patricia D.A.
Thébaud, Bernard
Archer, Stephen L.
The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)
title The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)
title_full The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)
title_fullStr The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)
title_full_unstemmed The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)
title_short The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)
title_sort comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hdasmc)
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717140/
https://www.ncbi.nlm.nih.gov/pubmed/35005134
http://dx.doi.org/10.1016/j.dib.2021.107736
work_keys_str_mv AT bentleyrachelet thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT hindmarchcharlesct thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT dunhamsnarykimberlyj thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT snetsingerbrooke thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT mewburnjeffreyd thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT thebaudarthur thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT limapatriciada thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT thebaudbernard thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT archerstephenl thecomprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT bentleyrachelet comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT hindmarchcharlesct comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT dunhamsnarykimberlyj comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT snetsingerbrooke comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT mewburnjeffreyd comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT thebaudarthur comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT limapatriciada comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT thebaudbernard comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc
AT archerstephenl comprehensivetranscriptomeofhumanductusarteriosussmoothmusclecellshdasmc