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Candidates for Balancing Selection in Leishmania donovani Complex Parasites

The Leishmania donovani species complex is the causative agent of visceral leishmaniasis, which cause 20–40,000 fatalities a year. Here, we conduct a screen for balancing selection in this species complex. We used 384 publicly available L. donovani and L. infantum genomes, and sequence 93 isolates o...

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Autores principales: Grace, Cooper Alastair, Forrester, Sarah, Silva, Vladimir Costa, Carvalho, Kátia Silene Sousa, Kilford, Hannah, Chew, Yen Peng, James, Sally, Costa, Dorcas L, Mottram, Jeremy C, Costa, Carlos C H N, Jeffares, Daniel C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717319/
https://www.ncbi.nlm.nih.gov/pubmed/34865011
http://dx.doi.org/10.1093/gbe/evab265
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author Grace, Cooper Alastair
Forrester, Sarah
Silva, Vladimir Costa
Carvalho, Kátia Silene Sousa
Kilford, Hannah
Chew, Yen Peng
James, Sally
Costa, Dorcas L
Mottram, Jeremy C
Costa, Carlos C H N
Jeffares, Daniel C
author_facet Grace, Cooper Alastair
Forrester, Sarah
Silva, Vladimir Costa
Carvalho, Kátia Silene Sousa
Kilford, Hannah
Chew, Yen Peng
James, Sally
Costa, Dorcas L
Mottram, Jeremy C
Costa, Carlos C H N
Jeffares, Daniel C
author_sort Grace, Cooper Alastair
collection PubMed
description The Leishmania donovani species complex is the causative agent of visceral leishmaniasis, which cause 20–40,000 fatalities a year. Here, we conduct a screen for balancing selection in this species complex. We used 384 publicly available L. donovani and L. infantum genomes, and sequence 93 isolates of L. infantum from Brazil to describe the global diversity of this species complex. We identify five genetically distinct populations that are sufficiently represented by genomic data to search for signatures of selection. We find that signals of balancing selection are generally not shared between populations, consistent with transient adaptive events, rather than long-term balancing selection. We then apply multiple diversity metrics to identify candidate genes with robust signatures of balancing selection, identifying a curated set of 24 genes with robust signatures. These include zeta toxin, nodulin-like, and flagellum attachment proteins. This study highlights the extent of genetic divergence between L. donovani complex parasites and provides genes for further study.
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spelling pubmed-87173192022-01-05 Candidates for Balancing Selection in Leishmania donovani Complex Parasites Grace, Cooper Alastair Forrester, Sarah Silva, Vladimir Costa Carvalho, Kátia Silene Sousa Kilford, Hannah Chew, Yen Peng James, Sally Costa, Dorcas L Mottram, Jeremy C Costa, Carlos C H N Jeffares, Daniel C Genome Biol Evol Research Article The Leishmania donovani species complex is the causative agent of visceral leishmaniasis, which cause 20–40,000 fatalities a year. Here, we conduct a screen for balancing selection in this species complex. We used 384 publicly available L. donovani and L. infantum genomes, and sequence 93 isolates of L. infantum from Brazil to describe the global diversity of this species complex. We identify five genetically distinct populations that are sufficiently represented by genomic data to search for signatures of selection. We find that signals of balancing selection are generally not shared between populations, consistent with transient adaptive events, rather than long-term balancing selection. We then apply multiple diversity metrics to identify candidate genes with robust signatures of balancing selection, identifying a curated set of 24 genes with robust signatures. These include zeta toxin, nodulin-like, and flagellum attachment proteins. This study highlights the extent of genetic divergence between L. donovani complex parasites and provides genes for further study. Oxford University Press 2021-12-02 /pmc/articles/PMC8717319/ /pubmed/34865011 http://dx.doi.org/10.1093/gbe/evab265 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Grace, Cooper Alastair
Forrester, Sarah
Silva, Vladimir Costa
Carvalho, Kátia Silene Sousa
Kilford, Hannah
Chew, Yen Peng
James, Sally
Costa, Dorcas L
Mottram, Jeremy C
Costa, Carlos C H N
Jeffares, Daniel C
Candidates for Balancing Selection in Leishmania donovani Complex Parasites
title Candidates for Balancing Selection in Leishmania donovani Complex Parasites
title_full Candidates for Balancing Selection in Leishmania donovani Complex Parasites
title_fullStr Candidates for Balancing Selection in Leishmania donovani Complex Parasites
title_full_unstemmed Candidates for Balancing Selection in Leishmania donovani Complex Parasites
title_short Candidates for Balancing Selection in Leishmania donovani Complex Parasites
title_sort candidates for balancing selection in leishmania donovani complex parasites
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717319/
https://www.ncbi.nlm.nih.gov/pubmed/34865011
http://dx.doi.org/10.1093/gbe/evab265
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