Cargando…

Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)

[Image: see text] The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter–coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-perform...

Descripción completa

Detalles Bibliográficos
Autores principales: dos Santos Silva, Norton Felipe, Willemart, Rodrigo Hirata, Cunha da Silva, José Roberto Machado, da Silva Junior, Pedro Ismael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717388/
https://www.ncbi.nlm.nih.gov/pubmed/34984257
http://dx.doi.org/10.1021/acsomega.1c02525
_version_ 1784624521644343296
author dos Santos Silva, Norton Felipe
Willemart, Rodrigo Hirata
Cunha da Silva, José Roberto Machado
da Silva Junior, Pedro Ismael
author_facet dos Santos Silva, Norton Felipe
Willemart, Rodrigo Hirata
Cunha da Silva, José Roberto Machado
da Silva Junior, Pedro Ismael
author_sort dos Santos Silva, Norton Felipe
collection PubMed
description [Image: see text] The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter–coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-performance liquid chromatography (RP-HPLC), polyacrylamide gel electrophoresis (PAGE), and nanoscale liquid chromatography coupled to mass spectrometry (nLC-MS/MS) analysis. Most of the fractions studied are hydrophobic, being proteins with molecular weights of ∼28, 62, and ∼198 kDa. These proteins seem to be homologous to proteins involved in product secretion, cytoskeleton, protein binding, cellular metabolism, and antimicrobial action among others. Lubricant function is also possible based on the literature. We were able to identify 147 proteins in the inner region, 91 proteins from the outer dorsal region, and 36 proteins from the outer ventral region. Some proteins are present only in one of these regions and some are shared by one or more regions. Our work provides, to the best of our knowledge, the first proteome characterization of the content of an arthrodial membrane gland in arachnids. Dataset Identifier: ftp://massive.ucsd.edu/MSV000087195/.
format Online
Article
Text
id pubmed-8717388
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-87173882022-01-03 Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones) dos Santos Silva, Norton Felipe Willemart, Rodrigo Hirata Cunha da Silva, José Roberto Machado da Silva Junior, Pedro Ismael ACS Omega [Image: see text] The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter–coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-performance liquid chromatography (RP-HPLC), polyacrylamide gel electrophoresis (PAGE), and nanoscale liquid chromatography coupled to mass spectrometry (nLC-MS/MS) analysis. Most of the fractions studied are hydrophobic, being proteins with molecular weights of ∼28, 62, and ∼198 kDa. These proteins seem to be homologous to proteins involved in product secretion, cytoskeleton, protein binding, cellular metabolism, and antimicrobial action among others. Lubricant function is also possible based on the literature. We were able to identify 147 proteins in the inner region, 91 proteins from the outer dorsal region, and 36 proteins from the outer ventral region. Some proteins are present only in one of these regions and some are shared by one or more regions. Our work provides, to the best of our knowledge, the first proteome characterization of the content of an arthrodial membrane gland in arachnids. Dataset Identifier: ftp://massive.ucsd.edu/MSV000087195/. American Chemical Society 2021-12-13 /pmc/articles/PMC8717388/ /pubmed/34984257 http://dx.doi.org/10.1021/acsomega.1c02525 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle dos Santos Silva, Norton Felipe
Willemart, Rodrigo Hirata
Cunha da Silva, José Roberto Machado
da Silva Junior, Pedro Ismael
Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)
title Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)
title_full Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)
title_fullStr Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)
title_full_unstemmed Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)
title_short Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)
title_sort protein components of the arthrodial membrane gland in a neotropical harvestman (arachnida, opiliones)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717388/
https://www.ncbi.nlm.nih.gov/pubmed/34984257
http://dx.doi.org/10.1021/acsomega.1c02525
work_keys_str_mv AT dossantossilvanortonfelipe proteincomponentsofthearthrodialmembraneglandinaneotropicalharvestmanarachnidaopiliones
AT willemartrodrigohirata proteincomponentsofthearthrodialmembraneglandinaneotropicalharvestmanarachnidaopiliones
AT cunhadasilvajoserobertomachado proteincomponentsofthearthrodialmembraneglandinaneotropicalharvestmanarachnidaopiliones
AT dasilvajuniorpedroismael proteincomponentsofthearthrodialmembraneglandinaneotropicalharvestmanarachnidaopiliones