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Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones)
[Image: see text] The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter–coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-perform...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717388/ https://www.ncbi.nlm.nih.gov/pubmed/34984257 http://dx.doi.org/10.1021/acsomega.1c02525 |
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author | dos Santos Silva, Norton Felipe Willemart, Rodrigo Hirata Cunha da Silva, José Roberto Machado da Silva Junior, Pedro Ismael |
author_facet | dos Santos Silva, Norton Felipe Willemart, Rodrigo Hirata Cunha da Silva, José Roberto Machado da Silva Junior, Pedro Ismael |
author_sort | dos Santos Silva, Norton Felipe |
collection | PubMed |
description | [Image: see text] The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter–coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-performance liquid chromatography (RP-HPLC), polyacrylamide gel electrophoresis (PAGE), and nanoscale liquid chromatography coupled to mass spectrometry (nLC-MS/MS) analysis. Most of the fractions studied are hydrophobic, being proteins with molecular weights of ∼28, 62, and ∼198 kDa. These proteins seem to be homologous to proteins involved in product secretion, cytoskeleton, protein binding, cellular metabolism, and antimicrobial action among others. Lubricant function is also possible based on the literature. We were able to identify 147 proteins in the inner region, 91 proteins from the outer dorsal region, and 36 proteins from the outer ventral region. Some proteins are present only in one of these regions and some are shared by one or more regions. Our work provides, to the best of our knowledge, the first proteome characterization of the content of an arthrodial membrane gland in arachnids. Dataset Identifier: ftp://massive.ucsd.edu/MSV000087195/. |
format | Online Article Text |
id | pubmed-8717388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-87173882022-01-03 Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones) dos Santos Silva, Norton Felipe Willemart, Rodrigo Hirata Cunha da Silva, José Roberto Machado da Silva Junior, Pedro Ismael ACS Omega [Image: see text] The content of arthrodial membrane glands in arthropods has seldom been studied. Here, we have analyzed the proteins of the arthrodial membrane gland of the trochanter–coxa articulation of the fourth pair of legs in the harvestman Mischonyx cuspidatus via reverse-phase high-performance liquid chromatography (RP-HPLC), polyacrylamide gel electrophoresis (PAGE), and nanoscale liquid chromatography coupled to mass spectrometry (nLC-MS/MS) analysis. Most of the fractions studied are hydrophobic, being proteins with molecular weights of ∼28, 62, and ∼198 kDa. These proteins seem to be homologous to proteins involved in product secretion, cytoskeleton, protein binding, cellular metabolism, and antimicrobial action among others. Lubricant function is also possible based on the literature. We were able to identify 147 proteins in the inner region, 91 proteins from the outer dorsal region, and 36 proteins from the outer ventral region. Some proteins are present only in one of these regions and some are shared by one or more regions. Our work provides, to the best of our knowledge, the first proteome characterization of the content of an arthrodial membrane gland in arachnids. Dataset Identifier: ftp://massive.ucsd.edu/MSV000087195/. American Chemical Society 2021-12-13 /pmc/articles/PMC8717388/ /pubmed/34984257 http://dx.doi.org/10.1021/acsomega.1c02525 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | dos Santos Silva, Norton Felipe Willemart, Rodrigo Hirata Cunha da Silva, José Roberto Machado da Silva Junior, Pedro Ismael Protein Components of the Arthrodial Membrane Gland in a Neotropical Harvestman (Arachnida, Opiliones) |
title | Protein Components of the Arthrodial Membrane Gland
in a Neotropical Harvestman (Arachnida, Opiliones) |
title_full | Protein Components of the Arthrodial Membrane Gland
in a Neotropical Harvestman (Arachnida, Opiliones) |
title_fullStr | Protein Components of the Arthrodial Membrane Gland
in a Neotropical Harvestman (Arachnida, Opiliones) |
title_full_unstemmed | Protein Components of the Arthrodial Membrane Gland
in a Neotropical Harvestman (Arachnida, Opiliones) |
title_short | Protein Components of the Arthrodial Membrane Gland
in a Neotropical Harvestman (Arachnida, Opiliones) |
title_sort | protein components of the arthrodial membrane gland
in a neotropical harvestman (arachnida, opiliones) |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717388/ https://www.ncbi.nlm.nih.gov/pubmed/34984257 http://dx.doi.org/10.1021/acsomega.1c02525 |
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