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Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production

[Image: see text] Lipase catalytic activity is greatly influenced by immobilization on nanoparticles. In this study, lipase from Aspergillus niger was immobilized on TiO(2) nanoparticles with different morphologies: microspheres, nanotubes, and nanosheets. All TiO(2) samples were prepared by a hydro...

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Autores principales: El-Kady, Kholoud, Raslan, Mai, Zaki, Ayman H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717535/
https://www.ncbi.nlm.nih.gov/pubmed/34984280
http://dx.doi.org/10.1021/acsomega.1c04942
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author El-Kady, Kholoud
Raslan, Mai
Zaki, Ayman H.
author_facet El-Kady, Kholoud
Raslan, Mai
Zaki, Ayman H.
author_sort El-Kady, Kholoud
collection PubMed
description [Image: see text] Lipase catalytic activity is greatly influenced by immobilization on nanoparticles. In this study, lipase from Aspergillus niger was immobilized on TiO(2) nanoparticles with different morphologies: microspheres, nanotubes, and nanosheets. All TiO(2) samples were prepared by a hydrothermal method. Lipase/TiO(2) nanocomposites were prepared by a physical adsorption method through hydrophobic interactions. The prepared composites were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM). The catalytic activity of free and immobilized lipases was tested using sunflower oil in the presence of methanol to produce biodiesel at 40 °C for 90 min. The lipase immobilized on TiO(2) microspheres showed the highest activity compared to the lipase immobilized on TiO(2) nanotubes and nanosheets. To optimize the lipase-to-microsphere ratio, lipase was immobilized on TiO(2) microspheres in different microspheres/lipase, w/w, (S/L) ratios of 1:1, 1:0.75, 1:0.5, and 1:0.25. It was noticed that the hydrolytic activity follows the order 1:0.25 > 1:0.5 > 1:75 > 1:1. The immobilization yield activities were found to be 113, 123, 125, and 130% for the microspheres/lipase (S/L) ratios of 1:1, 1:0.75, 1:0.5, and 1:0.25, respectively.
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spelling pubmed-87175352022-01-03 Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production El-Kady, Kholoud Raslan, Mai Zaki, Ayman H. ACS Omega [Image: see text] Lipase catalytic activity is greatly influenced by immobilization on nanoparticles. In this study, lipase from Aspergillus niger was immobilized on TiO(2) nanoparticles with different morphologies: microspheres, nanotubes, and nanosheets. All TiO(2) samples were prepared by a hydrothermal method. Lipase/TiO(2) nanocomposites were prepared by a physical adsorption method through hydrophobic interactions. The prepared composites were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM). The catalytic activity of free and immobilized lipases was tested using sunflower oil in the presence of methanol to produce biodiesel at 40 °C for 90 min. The lipase immobilized on TiO(2) microspheres showed the highest activity compared to the lipase immobilized on TiO(2) nanotubes and nanosheets. To optimize the lipase-to-microsphere ratio, lipase was immobilized on TiO(2) microspheres in different microspheres/lipase, w/w, (S/L) ratios of 1:1, 1:0.75, 1:0.5, and 1:0.25. It was noticed that the hydrolytic activity follows the order 1:0.25 > 1:0.5 > 1:75 > 1:1. The immobilization yield activities were found to be 113, 123, 125, and 130% for the microspheres/lipase (S/L) ratios of 1:1, 1:0.75, 1:0.5, and 1:0.25, respectively. American Chemical Society 2021-12-14 /pmc/articles/PMC8717535/ /pubmed/34984280 http://dx.doi.org/10.1021/acsomega.1c04942 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle El-Kady, Kholoud
Raslan, Mai
Zaki, Ayman H.
Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production
title Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production
title_full Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production
title_fullStr Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production
title_full_unstemmed Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production
title_short Effect of Different TiO(2) Morphologies on the Activity of Immobilized Lipase for Biodiesel Production
title_sort effect of different tio(2) morphologies on the activity of immobilized lipase for biodiesel production
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717535/
https://www.ncbi.nlm.nih.gov/pubmed/34984280
http://dx.doi.org/10.1021/acsomega.1c04942
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