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Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels

Gelatin methacryloyl (GelMA) hydrogels have aroused considerable interests in the field of tissue engineering due to tunable physical properties and cell response parameters. A number of works have studied the impact of GelMA concentration, photo-initiator concentration, methacrylic anhydride (MA) c...

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Autores principales: Liu, Taotao, Zhang, Yuzhuo, Sun, Mingyue, Jin, Meiqi, Xia, Wei, Yang, Huazhe, Wang, Tianlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717941/
https://www.ncbi.nlm.nih.gov/pubmed/34976995
http://dx.doi.org/10.3389/fbioe.2021.810155
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author Liu, Taotao
Zhang, Yuzhuo
Sun, Mingyue
Jin, Meiqi
Xia, Wei
Yang, Huazhe
Wang, Tianlin
author_facet Liu, Taotao
Zhang, Yuzhuo
Sun, Mingyue
Jin, Meiqi
Xia, Wei
Yang, Huazhe
Wang, Tianlin
author_sort Liu, Taotao
collection PubMed
description Gelatin methacryloyl (GelMA) hydrogels have aroused considerable interests in the field of tissue engineering due to tunable physical properties and cell response parameters. A number of works have studied the impact of GelMA concentration, photo-initiator concentration, methacrylic anhydride (MA) concentration, cooling rate and temperature gradient on GelMA hydrogel generation, but little attention has been paid to the effect of the freezing temperatures and freezing time of GelMA prepolymer solution during preparation. In this study, GelMA hydrogels were synthesized with different freezing temperatures and time. It was found that the lower freezing temperatures and longer freezing time caused smaller pore sizes that realized higher cell viability and proliferation of MC3T3-E1 cells. The results showed that tunable microstructure of GelMA could be achieved by regulating the freezing conditions of GelMA, which provided a broad prospect for the applications of GelMA hydrogels in tissue engineering.
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spelling pubmed-87179412021-12-31 Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels Liu, Taotao Zhang, Yuzhuo Sun, Mingyue Jin, Meiqi Xia, Wei Yang, Huazhe Wang, Tianlin Front Bioeng Biotechnol Bioengineering and Biotechnology Gelatin methacryloyl (GelMA) hydrogels have aroused considerable interests in the field of tissue engineering due to tunable physical properties and cell response parameters. A number of works have studied the impact of GelMA concentration, photo-initiator concentration, methacrylic anhydride (MA) concentration, cooling rate and temperature gradient on GelMA hydrogel generation, but little attention has been paid to the effect of the freezing temperatures and freezing time of GelMA prepolymer solution during preparation. In this study, GelMA hydrogels were synthesized with different freezing temperatures and time. It was found that the lower freezing temperatures and longer freezing time caused smaller pore sizes that realized higher cell viability and proliferation of MC3T3-E1 cells. The results showed that tunable microstructure of GelMA could be achieved by regulating the freezing conditions of GelMA, which provided a broad prospect for the applications of GelMA hydrogels in tissue engineering. Frontiers Media S.A. 2021-12-14 /pmc/articles/PMC8717941/ /pubmed/34976995 http://dx.doi.org/10.3389/fbioe.2021.810155 Text en Copyright © 2021 Liu, Zhang, Sun, Jin, Xia, Yang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liu, Taotao
Zhang, Yuzhuo
Sun, Mingyue
Jin, Meiqi
Xia, Wei
Yang, Huazhe
Wang, Tianlin
Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels
title Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels
title_full Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels
title_fullStr Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels
title_full_unstemmed Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels
title_short Effect of Freezing Process on the Microstructure of Gelatin Methacryloyl Hydrogels
title_sort effect of freezing process on the microstructure of gelatin methacryloyl hydrogels
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8717941/
https://www.ncbi.nlm.nih.gov/pubmed/34976995
http://dx.doi.org/10.3389/fbioe.2021.810155
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