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The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study

Generally, intestinal microbiota can be classified into intestinal cavity microbiota and mucosal microbiota, among which, the former is the default type. This study aimed to identify the differences between fecal microbiota and intestinal fluid microbiota in colon polys. This study enrolled patients...

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Autores principales: Zhou, Xi, Zhang, Shuoqiu, Liu, Dan, Qian, Haihua, Zhang, Dan, Liu, Qiuhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718174/
https://www.ncbi.nlm.nih.gov/pubmed/34967350
http://dx.doi.org/10.1097/MD.0000000000028028
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author Zhou, Xi
Zhang, Shuoqiu
Liu, Dan
Qian, Haihua
Zhang, Dan
Liu, Qiuhui
author_facet Zhou, Xi
Zhang, Shuoqiu
Liu, Dan
Qian, Haihua
Zhang, Dan
Liu, Qiuhui
author_sort Zhou, Xi
collection PubMed
description Generally, intestinal microbiota can be classified into intestinal cavity microbiota and mucosal microbiota, among which, the former is the default type. This study aimed to identify the differences between fecal microbiota and intestinal fluid microbiota in colon polys. This study enrolled patients with colon polys who met the Rome-III criteria to carry out 16s rDNA gene sequencing. Then, both fresh feces as well as intestinal fluid was sampled. Thereafter, α/β diversities, together with the heterogeneities with regard to microbial function and structure were assessed among those intestinal fluid and fresh feces samples collected. According to bioinformatics analysis, difference in α-diversity was not statistically significant between intestinal fluid microbiota and fecal microbiota among patients with colorectal polyps (CPs). Non-metric multidimensional scaling analysis of β-diversity revealed that differences were of statistical significance between both groups. In addition, linear discriminant analysis effect size analysis displayed great heterogeneities in intestinal microbiota of both groups, including Firmicutes, Clostridia, and Phascolarctobacterium. At the phylum level, difference (P = .016) in Spirochaetes was statistically significant between the intestinal fluid group and fecal group. At the family level, differences in Bacteroidaceae, Micrococcaceae, F16, Spirocheatacae, Enterobacteriaceae, Cardiobacteriaceae, Turkish Spirobacteriaceae, Bifidobacteriaceae, and Dethiosulfovibrionaceae were statistically significant between the 2 groups. At the genus level, there were statistical differences between the 2 groups in terms of Bacteroidetes, Rothia, Actinobacillus, F16, Treponema, Oscillospira, Turicibacter, Sharpea, Heamophilus, Veillonella, and Cardiobacterium. There are statistical differences in the composition between intestinal microbiota and fecal microbiota in CP patients, both of which are equally important and indispensable for analyzing the intestinal microbiota in CP patients.
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spelling pubmed-87181742022-01-03 The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study Zhou, Xi Zhang, Shuoqiu Liu, Dan Qian, Haihua Zhang, Dan Liu, Qiuhui Medicine (Baltimore) 4500 Generally, intestinal microbiota can be classified into intestinal cavity microbiota and mucosal microbiota, among which, the former is the default type. This study aimed to identify the differences between fecal microbiota and intestinal fluid microbiota in colon polys. This study enrolled patients with colon polys who met the Rome-III criteria to carry out 16s rDNA gene sequencing. Then, both fresh feces as well as intestinal fluid was sampled. Thereafter, α/β diversities, together with the heterogeneities with regard to microbial function and structure were assessed among those intestinal fluid and fresh feces samples collected. According to bioinformatics analysis, difference in α-diversity was not statistically significant between intestinal fluid microbiota and fecal microbiota among patients with colorectal polyps (CPs). Non-metric multidimensional scaling analysis of β-diversity revealed that differences were of statistical significance between both groups. In addition, linear discriminant analysis effect size analysis displayed great heterogeneities in intestinal microbiota of both groups, including Firmicutes, Clostridia, and Phascolarctobacterium. At the phylum level, difference (P = .016) in Spirochaetes was statistically significant between the intestinal fluid group and fecal group. At the family level, differences in Bacteroidaceae, Micrococcaceae, F16, Spirocheatacae, Enterobacteriaceae, Cardiobacteriaceae, Turkish Spirobacteriaceae, Bifidobacteriaceae, and Dethiosulfovibrionaceae were statistically significant between the 2 groups. At the genus level, there were statistical differences between the 2 groups in terms of Bacteroidetes, Rothia, Actinobacillus, F16, Treponema, Oscillospira, Turicibacter, Sharpea, Heamophilus, Veillonella, and Cardiobacterium. There are statistical differences in the composition between intestinal microbiota and fecal microbiota in CP patients, both of which are equally important and indispensable for analyzing the intestinal microbiota in CP patients. Lippincott Williams & Wilkins 2021-12-30 /pmc/articles/PMC8718174/ /pubmed/34967350 http://dx.doi.org/10.1097/MD.0000000000028028 Text en Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle 4500
Zhou, Xi
Zhang, Shuoqiu
Liu, Dan
Qian, Haihua
Zhang, Dan
Liu, Qiuhui
The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study
title The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study
title_full The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study
title_fullStr The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study
title_full_unstemmed The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study
title_short The differences between fecal microbiota and intestinal fluid microbiota in colon polyps: An observational study
title_sort differences between fecal microbiota and intestinal fluid microbiota in colon polyps: an observational study
topic 4500
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718174/
https://www.ncbi.nlm.nih.gov/pubmed/34967350
http://dx.doi.org/10.1097/MD.0000000000028028
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