Cargando…
Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae
Investigations of phytoplankton responses to iron stress in seawater are complicated by the fact that iron concentrations do not necessarily reflect bioavailability. Most studies to date have been based on single species or field samples and are problematic to interpret. Here, we report results from...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718654/ https://www.ncbi.nlm.nih.gov/pubmed/35024100 http://dx.doi.org/10.1016/j.csbj.2021.12.023 |
_version_ | 1784624774178144256 |
---|---|
author | Malych, Ronald Stopka, Pavel Mach, Jan Kotabová, Eva Prášil, Ondřej Sutak, Robert |
author_facet | Malych, Ronald Stopka, Pavel Mach, Jan Kotabová, Eva Prášil, Ondřej Sutak, Robert |
author_sort | Malych, Ronald |
collection | PubMed |
description | Investigations of phytoplankton responses to iron stress in seawater are complicated by the fact that iron concentrations do not necessarily reflect bioavailability. Most studies to date have been based on single species or field samples and are problematic to interpret. Here, we report results from an experimental cocultivation model system that enabled us to evaluate interspecific competition as a function of iron content and form, and to study the effect of nutritional conditions on the proteomic profiles of individual species. Our study revealed that the dinoflagellate Amphidinium carterae was able to utilize iron from a hydroxamate siderophore, a strategy that could provide an ecological advantage in environments where siderophores present an important source of iron. Additionally, proteomic analysis allowed us to identify a potential candidate protein involved in iron acquisition from hydroxamate siderophores, a strategy that is largely unknown in eukaryotic phytoplankton. |
format | Online Article Text |
id | pubmed-8718654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Research Network of Computational and Structural Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-87186542022-01-11 Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae Malych, Ronald Stopka, Pavel Mach, Jan Kotabová, Eva Prášil, Ondřej Sutak, Robert Comput Struct Biotechnol J Research Article Investigations of phytoplankton responses to iron stress in seawater are complicated by the fact that iron concentrations do not necessarily reflect bioavailability. Most studies to date have been based on single species or field samples and are problematic to interpret. Here, we report results from an experimental cocultivation model system that enabled us to evaluate interspecific competition as a function of iron content and form, and to study the effect of nutritional conditions on the proteomic profiles of individual species. Our study revealed that the dinoflagellate Amphidinium carterae was able to utilize iron from a hydroxamate siderophore, a strategy that could provide an ecological advantage in environments where siderophores present an important source of iron. Additionally, proteomic analysis allowed us to identify a potential candidate protein involved in iron acquisition from hydroxamate siderophores, a strategy that is largely unknown in eukaryotic phytoplankton. Research Network of Computational and Structural Biotechnology 2021-12-20 /pmc/articles/PMC8718654/ /pubmed/35024100 http://dx.doi.org/10.1016/j.csbj.2021.12.023 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Malych, Ronald Stopka, Pavel Mach, Jan Kotabová, Eva Prášil, Ondřej Sutak, Robert Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae |
title | Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae |
title_full | Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae |
title_fullStr | Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae |
title_full_unstemmed | Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae |
title_short | Flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate Amphidinium carterae |
title_sort | flow cytometry-based study of model marine microalgal consortia revealed an ecological advantage of siderophore utilization by the dinoflagellate amphidinium carterae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718654/ https://www.ncbi.nlm.nih.gov/pubmed/35024100 http://dx.doi.org/10.1016/j.csbj.2021.12.023 |
work_keys_str_mv | AT malychronald flowcytometrybasedstudyofmodelmarinemicroalgalconsortiarevealedanecologicaladvantageofsiderophoreutilizationbythedinoflagellateamphidiniumcarterae AT stopkapavel flowcytometrybasedstudyofmodelmarinemicroalgalconsortiarevealedanecologicaladvantageofsiderophoreutilizationbythedinoflagellateamphidiniumcarterae AT machjan flowcytometrybasedstudyofmodelmarinemicroalgalconsortiarevealedanecologicaladvantageofsiderophoreutilizationbythedinoflagellateamphidiniumcarterae AT kotabovaeva flowcytometrybasedstudyofmodelmarinemicroalgalconsortiarevealedanecologicaladvantageofsiderophoreutilizationbythedinoflagellateamphidiniumcarterae AT prasilondrej flowcytometrybasedstudyofmodelmarinemicroalgalconsortiarevealedanecologicaladvantageofsiderophoreutilizationbythedinoflagellateamphidiniumcarterae AT sutakrobert flowcytometrybasedstudyofmodelmarinemicroalgalconsortiarevealedanecologicaladvantageofsiderophoreutilizationbythedinoflagellateamphidiniumcarterae |