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Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities

Many species of seed-borne fungi are closely allied with seed varieties and growing regions, including many seed-borne pathogens, but their species richness and distribution remain largely unknown. This study was conducted to explore the seed-borne fungal composition, abundance and diversity in Aven...

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Autores principales: Wang, Jianjun, Wei, Xuekai, Chen, Taixiang, White, James F., Zhao, Guiqin, Li, Chunjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718926/
https://www.ncbi.nlm.nih.gov/pubmed/34975777
http://dx.doi.org/10.3389/fmicb.2021.724999
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author Wang, Jianjun
Wei, Xuekai
Chen, Taixiang
White, James F.
Zhao, Guiqin
Li, Chunjie
author_facet Wang, Jianjun
Wei, Xuekai
Chen, Taixiang
White, James F.
Zhao, Guiqin
Li, Chunjie
author_sort Wang, Jianjun
collection PubMed
description Many species of seed-borne fungi are closely allied with seed varieties and growing regions, including many seed-borne pathogens, but their species richness and distribution remain largely unknown. This study was conducted to explore the seed-borne fungal composition, abundance and diversity in Avena sativa (B7) and A. nuda (B2) seed samples collected from Baicheng (BB), Dingxi (DB) and Haibei (HB) city, using Illumina sequencing techniques. Our results show that a total of 543,707 sequences were obtained and these were assigned to 244 operational taxonomic units (OTUs) with 97% similarity. Oat varieties and growing locations had a significant difference on seed-borne fungal diversity. HB had a higher fungal diversity than BB and DB, Shannon diversity and ACE richness index of fungal in HB seeds was significantly higher than in BB and DB (P < 0.05). In different varieties, both taxon richness and evenness of B7 seeds was significantly higher than B2 (P < 0.05). A total of 4 fungal phyla and 26 fungal genera were detected. Ascomycota was the dominant phylum and Alternaria sp. was the most abundant genus in B2 and B7 oat seeds from different regions. Mycosphaerella sp. had a higher abundance in HB7 and DB7, respectively, Epicoccum sp. had a higher abundance in HB7 and BB7. The results of alpha and beta diversity analysis revealed the presence of different effects in fungal communities of different varieties and regions of oat, especially in seed pathogenic fungi distribution. Structural equation modeling also explained oat varieties and growing regions have significant influences on seed-borne fungal abundance, composition and diversity. This study demonstrated that the differences of varieties and regions are the main factors resulting in the changes of seed-borne fungal community of oat.
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spelling pubmed-87189262022-01-01 Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities Wang, Jianjun Wei, Xuekai Chen, Taixiang White, James F. Zhao, Guiqin Li, Chunjie Front Microbiol Microbiology Many species of seed-borne fungi are closely allied with seed varieties and growing regions, including many seed-borne pathogens, but their species richness and distribution remain largely unknown. This study was conducted to explore the seed-borne fungal composition, abundance and diversity in Avena sativa (B7) and A. nuda (B2) seed samples collected from Baicheng (BB), Dingxi (DB) and Haibei (HB) city, using Illumina sequencing techniques. Our results show that a total of 543,707 sequences were obtained and these were assigned to 244 operational taxonomic units (OTUs) with 97% similarity. Oat varieties and growing locations had a significant difference on seed-borne fungal diversity. HB had a higher fungal diversity than BB and DB, Shannon diversity and ACE richness index of fungal in HB seeds was significantly higher than in BB and DB (P < 0.05). In different varieties, both taxon richness and evenness of B7 seeds was significantly higher than B2 (P < 0.05). A total of 4 fungal phyla and 26 fungal genera were detected. Ascomycota was the dominant phylum and Alternaria sp. was the most abundant genus in B2 and B7 oat seeds from different regions. Mycosphaerella sp. had a higher abundance in HB7 and DB7, respectively, Epicoccum sp. had a higher abundance in HB7 and BB7. The results of alpha and beta diversity analysis revealed the presence of different effects in fungal communities of different varieties and regions of oat, especially in seed pathogenic fungi distribution. Structural equation modeling also explained oat varieties and growing regions have significant influences on seed-borne fungal abundance, composition and diversity. This study demonstrated that the differences of varieties and regions are the main factors resulting in the changes of seed-borne fungal community of oat. Frontiers Media S.A. 2021-12-17 /pmc/articles/PMC8718926/ /pubmed/34975777 http://dx.doi.org/10.3389/fmicb.2021.724999 Text en Copyright © 2021 Wang, Wei, Chen, White, Zhao and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Jianjun
Wei, Xuekai
Chen, Taixiang
White, James F.
Zhao, Guiqin
Li, Chunjie
Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities
title Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities
title_full Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities
title_fullStr Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities
title_full_unstemmed Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities
title_short Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities
title_sort effects of oat varieties and growing locations on seed-borne fungal communities
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718926/
https://www.ncbi.nlm.nih.gov/pubmed/34975777
http://dx.doi.org/10.3389/fmicb.2021.724999
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