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Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain

Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutati...

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Autores principales: Xu, Xiao-Jie, Zhu, Qing, Jiang, Shao-Yan, Yan, Zhi-Yong, Geng, Chao, Tian, Yan-Ping, Li, Xiang-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718998/
https://www.ncbi.nlm.nih.gov/pubmed/34975975
http://dx.doi.org/10.3389/fpls.2021.788963
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author Xu, Xiao-Jie
Zhu, Qing
Jiang, Shao-Yan
Yan, Zhi-Yong
Geng, Chao
Tian, Yan-Ping
Li, Xiang-Dong
author_facet Xu, Xiao-Jie
Zhu, Qing
Jiang, Shao-Yan
Yan, Zhi-Yong
Geng, Chao
Tian, Yan-Ping
Li, Xiang-Dong
author_sort Xu, Xiao-Jie
collection PubMed
description Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutation is one of the challenging problems affecting their application in cross-protection. In this study, we found that the substitution of cysteine (C) at positions 57 or 60 in the zinc finger-like motif of HC-Pro with alanine (A; C57A or C60A) significantly reduced its RNA silencing suppression activity and SCMV virulence. To reduce the risk of mild strains mutating to virulent ones by reverse or complementary mutations, we obtained attenuated SCMV mutants with double-mutations in the zinc finger-like and FRNK motifs of HC-Pro and evaluated their potential application in cross-protection. The results showed that the maize plants infected with FKNK/C60A double-mutant showed symptomless until 95 days post-inoculation and FKNK/C60A cross-protected plants displayed high resistance to severe SCMV strain. This study provides theoretical and material bases for the control of SCMV through cross-protection.
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spelling pubmed-87189982022-01-01 Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain Xu, Xiao-Jie Zhu, Qing Jiang, Shao-Yan Yan, Zhi-Yong Geng, Chao Tian, Yan-Ping Li, Xiang-Dong Front Plant Sci Plant Science Sugarcane mosaic virus (SCMV; genus Potyvirus) induces maize dwarf mosaic disease that has caused serious yield losses of maize in China. Cross-protection is one of the efficient strategies to fight against severe virus strains. Although many mild strains have been identified, the spontaneous mutation is one of the challenging problems affecting their application in cross-protection. In this study, we found that the substitution of cysteine (C) at positions 57 or 60 in the zinc finger-like motif of HC-Pro with alanine (A; C57A or C60A) significantly reduced its RNA silencing suppression activity and SCMV virulence. To reduce the risk of mild strains mutating to virulent ones by reverse or complementary mutations, we obtained attenuated SCMV mutants with double-mutations in the zinc finger-like and FRNK motifs of HC-Pro and evaluated their potential application in cross-protection. The results showed that the maize plants infected with FKNK/C60A double-mutant showed symptomless until 95 days post-inoculation and FKNK/C60A cross-protected plants displayed high resistance to severe SCMV strain. This study provides theoretical and material bases for the control of SCMV through cross-protection. Frontiers Media S.A. 2021-12-17 /pmc/articles/PMC8718998/ /pubmed/34975975 http://dx.doi.org/10.3389/fpls.2021.788963 Text en Copyright © 2021 Xu, Zhu, Jiang, Yan, Geng, Tian and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xu, Xiao-Jie
Zhu, Qing
Jiang, Shao-Yan
Yan, Zhi-Yong
Geng, Chao
Tian, Yan-Ping
Li, Xiang-Dong
Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain
title Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain
title_full Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain
title_fullStr Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain
title_full_unstemmed Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain
title_short Development and Evaluation of Stable Sugarcane Mosaic Virus Mild Mutants for Cross-Protection Against Infection by Severe Strain
title_sort development and evaluation of stable sugarcane mosaic virus mild mutants for cross-protection against infection by severe strain
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8718998/
https://www.ncbi.nlm.nih.gov/pubmed/34975975
http://dx.doi.org/10.3389/fpls.2021.788963
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