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Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage
In this work, we characterized a novel chromosome-encoded AmpC β-lactamase gene, bla(PRC–1), in an isolate of a newly classified Pseudomonas species designated Pseudomonas wenzhouensis A20, which was isolated from sewage discharged from an animal farm in Wenzhou, China. Susceptibility testing, molec...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719060/ https://www.ncbi.nlm.nih.gov/pubmed/34975778 http://dx.doi.org/10.3389/fmicb.2021.732932 |
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author | Zhang, Peiyao Dong, Xu Zhou, Kexin Zhu, Tingting Liang, Jialei Shi, Weina Gao, Mengdi Feng, Chunlin Li, Qiaoling Zhang, Xueya Ren, Ping Lu, Junwan Lin, Xi Li, Kewei Zhu, Mei Bao, Qiyu Zhang, Hailin |
author_facet | Zhang, Peiyao Dong, Xu Zhou, Kexin Zhu, Tingting Liang, Jialei Shi, Weina Gao, Mengdi Feng, Chunlin Li, Qiaoling Zhang, Xueya Ren, Ping Lu, Junwan Lin, Xi Li, Kewei Zhu, Mei Bao, Qiyu Zhang, Hailin |
author_sort | Zhang, Peiyao |
collection | PubMed |
description | In this work, we characterized a novel chromosome-encoded AmpC β-lactamase gene, bla(PRC–1), in an isolate of a newly classified Pseudomonas species designated Pseudomonas wenzhouensis A20, which was isolated from sewage discharged from an animal farm in Wenzhou, China. Susceptibility testing, molecular cloning, and enzyme kinetic parameter analysis were performed to determine the function and enzymatic properties of the β-lactamase. Sequencing and comparative genomic analysis were conducted to clarify the phylogenetic relationship and genetic context of the bla(PRC–1) gene. PRC-1 is a 379-amino acid AmpC β-lactamase with a molecular weight of 41.48 kDa and a predicted pI of 6.44, sharing the highest amino acid identity (57.7%) with the functionally characterized AmpC β-lactamase PDC-211 (ARX71249). bla(PRC–1) confers resistance to many β-lactam antibiotics, including penicillins (penicillin G, amoxicillin, and amoxicillin-clavulanic acid) and cephalosporins (cefazolin, ceftriaxone, and cefotaxime). The kinetic properties of PRC-1 were compatible with those of a typical class C β-lactamase showing hydrolytic activities against β-lactam antibiotics, and the hydrolytic activity was strongly inhibited by avibactam. The genetic context of bla(PRC–1) was relatively conserved, and no mobile genetic element was predicted in its surrounding region. Identification of a novel β-lactamase gene in an unusual environmental bacterium reveals that there might be numerous unknown resistance mechanisms in bacterial populations, which may pose potential risks to human health due to universal horizontal gene transfer between microorganisms. It is therefore of great value to carry out extensive research on the mechanism of antibiotic resistance. |
format | Online Article Text |
id | pubmed-8719060 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87190602022-01-01 Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage Zhang, Peiyao Dong, Xu Zhou, Kexin Zhu, Tingting Liang, Jialei Shi, Weina Gao, Mengdi Feng, Chunlin Li, Qiaoling Zhang, Xueya Ren, Ping Lu, Junwan Lin, Xi Li, Kewei Zhu, Mei Bao, Qiyu Zhang, Hailin Front Microbiol Microbiology In this work, we characterized a novel chromosome-encoded AmpC β-lactamase gene, bla(PRC–1), in an isolate of a newly classified Pseudomonas species designated Pseudomonas wenzhouensis A20, which was isolated from sewage discharged from an animal farm in Wenzhou, China. Susceptibility testing, molecular cloning, and enzyme kinetic parameter analysis were performed to determine the function and enzymatic properties of the β-lactamase. Sequencing and comparative genomic analysis were conducted to clarify the phylogenetic relationship and genetic context of the bla(PRC–1) gene. PRC-1 is a 379-amino acid AmpC β-lactamase with a molecular weight of 41.48 kDa and a predicted pI of 6.44, sharing the highest amino acid identity (57.7%) with the functionally characterized AmpC β-lactamase PDC-211 (ARX71249). bla(PRC–1) confers resistance to many β-lactam antibiotics, including penicillins (penicillin G, amoxicillin, and amoxicillin-clavulanic acid) and cephalosporins (cefazolin, ceftriaxone, and cefotaxime). The kinetic properties of PRC-1 were compatible with those of a typical class C β-lactamase showing hydrolytic activities against β-lactam antibiotics, and the hydrolytic activity was strongly inhibited by avibactam. The genetic context of bla(PRC–1) was relatively conserved, and no mobile genetic element was predicted in its surrounding region. Identification of a novel β-lactamase gene in an unusual environmental bacterium reveals that there might be numerous unknown resistance mechanisms in bacterial populations, which may pose potential risks to human health due to universal horizontal gene transfer between microorganisms. It is therefore of great value to carry out extensive research on the mechanism of antibiotic resistance. Frontiers Media S.A. 2021-12-17 /pmc/articles/PMC8719060/ /pubmed/34975778 http://dx.doi.org/10.3389/fmicb.2021.732932 Text en Copyright © 2021 Zhang, Dong, Zhou, Zhu, Liang, Shi, Gao, Feng, Li, Zhang, Ren, Lu, Lin, Li, Zhu, Bao and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Peiyao Dong, Xu Zhou, Kexin Zhu, Tingting Liang, Jialei Shi, Weina Gao, Mengdi Feng, Chunlin Li, Qiaoling Zhang, Xueya Ren, Ping Lu, Junwan Lin, Xi Li, Kewei Zhu, Mei Bao, Qiyu Zhang, Hailin Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage |
title | Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage |
title_full | Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage |
title_fullStr | Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage |
title_full_unstemmed | Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage |
title_short | Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, bla(PRC–1), in an Isolate of a Newly Classified Pseudomonas Species, Pseudomonas wenzhouensis A20, From Animal Farm Sewage |
title_sort | characterization of a novel chromosome-encoded ampc β-lactamase gene, bla(prc–1), in an isolate of a newly classified pseudomonas species, pseudomonas wenzhouensis a20, from animal farm sewage |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719060/ https://www.ncbi.nlm.nih.gov/pubmed/34975778 http://dx.doi.org/10.3389/fmicb.2021.732932 |
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