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Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species

This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming...

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Autores principales: Zoccolotti, Jacqueline de Oliveira, Cavalheiro, Alberto José, Tasso, Camilla Olga, Ribas, Beatriz Ribeiro, Ferrisse, Túlio Morandin, Jorge, Janaina Habib
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719776/
https://www.ncbi.nlm.nih.gov/pubmed/34972152
http://dx.doi.org/10.1371/journal.pone.0261884
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author Zoccolotti, Jacqueline de Oliveira
Cavalheiro, Alberto José
Tasso, Camilla Olga
Ribas, Beatriz Ribeiro
Ferrisse, Túlio Morandin
Jorge, Janaina Habib
author_facet Zoccolotti, Jacqueline de Oliveira
Cavalheiro, Alberto José
Tasso, Camilla Olga
Ribas, Beatriz Ribeiro
Ferrisse, Túlio Morandin
Jorge, Janaina Habib
author_sort Zoccolotti, Jacqueline de Oliveira
collection PubMed
description This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.
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spelling pubmed-87197762022-01-01 Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species Zoccolotti, Jacqueline de Oliveira Cavalheiro, Alberto José Tasso, Camilla Olga Ribas, Beatriz Ribeiro Ferrisse, Túlio Morandin Jorge, Janaina Habib PLoS One Research Article This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells. Public Library of Science 2021-12-31 /pmc/articles/PMC8719776/ /pubmed/34972152 http://dx.doi.org/10.1371/journal.pone.0261884 Text en © 2021 Zoccolotti et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zoccolotti, Jacqueline de Oliveira
Cavalheiro, Alberto José
Tasso, Camilla Olga
Ribas, Beatriz Ribeiro
Ferrisse, Túlio Morandin
Jorge, Janaina Habib
Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species
title Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species
title_full Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species
title_fullStr Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species
title_full_unstemmed Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species
title_short Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species
title_sort antimicrobial efficacy and biocompatibility of extracts from cryptocarya species
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719776/
https://www.ncbi.nlm.nih.gov/pubmed/34972152
http://dx.doi.org/10.1371/journal.pone.0261884
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