Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an impr...

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Detalles Bibliográficos
Autores principales: Gutiérrez-Gálvez, Laura, del Caño, Rafael, Menéndez-Luque, Iris, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Pineda, Teresa, Pariente, Félix, García-Mendiola, Tania, Lorenzo, Encarnación
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719920/
https://www.ncbi.nlm.nih.gov/pubmed/34998140
http://dx.doi.org/10.1016/j.talanta.2021.123203
Descripción
Sumario:This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)(3)](2+) anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)(3)](2+)/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.

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