Cargando…

Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an impr...

Descripción completa

Detalles Bibliográficos
Autores principales: Gutiérrez-Gálvez, Laura, del Caño, Rafael, Menéndez-Luque, Iris, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Pineda, Teresa, Pariente, Félix, García-Mendiola, Tania, Lorenzo, Encarnación
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719920/
https://www.ncbi.nlm.nih.gov/pubmed/34998140
http://dx.doi.org/10.1016/j.talanta.2021.123203
_version_ 1784625039433269248
author Gutiérrez-Gálvez, Laura
del Caño, Rafael
Menéndez-Luque, Iris
García-Nieto, Daniel
Rodríguez-Peña, Micaela
Luna, Mónica
Pineda, Teresa
Pariente, Félix
García-Mendiola, Tania
Lorenzo, Encarnación
author_facet Gutiérrez-Gálvez, Laura
del Caño, Rafael
Menéndez-Luque, Iris
García-Nieto, Daniel
Rodríguez-Peña, Micaela
Luna, Mónica
Pineda, Teresa
Pariente, Félix
García-Mendiola, Tania
Lorenzo, Encarnación
author_sort Gutiérrez-Gálvez, Laura
collection PubMed
description This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)(3)](2+) anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)(3)](2+)/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM.
format Online
Article
Text
id pubmed-8719920
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-87199202022-01-03 Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection Gutiérrez-Gálvez, Laura del Caño, Rafael Menéndez-Luque, Iris García-Nieto, Daniel Rodríguez-Peña, Micaela Luna, Mónica Pineda, Teresa Pariente, Félix García-Mendiola, Tania Lorenzo, Encarnación Talanta Article This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)(3)](2+) anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)(3)](2+)/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM. Elsevier B.V. 2022-04-01 2022-01-01 /pmc/articles/PMC8719920/ /pubmed/34998140 http://dx.doi.org/10.1016/j.talanta.2021.123203 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Gutiérrez-Gálvez, Laura
del Caño, Rafael
Menéndez-Luque, Iris
García-Nieto, Daniel
Rodríguez-Peña, Micaela
Luna, Mónica
Pineda, Teresa
Pariente, Félix
García-Mendiola, Tania
Lorenzo, Encarnación
Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
title Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
title_full Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
title_fullStr Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
title_full_unstemmed Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
title_short Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection
title_sort electrochemiluminescent nanostructured dna biosensor for sars-cov-2 detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719920/
https://www.ncbi.nlm.nih.gov/pubmed/34998140
http://dx.doi.org/10.1016/j.talanta.2021.123203
work_keys_str_mv AT gutierrezgalvezlaura electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT delcanorafael electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT menendezluqueiris electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT garcianietodaniel electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT rodriguezpenamicaela electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT lunamonica electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT pinedateresa electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT parientefelix electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT garciamendiolatania electrochemiluminescentnanostructureddnabiosensorforsarscov2detection
AT lorenzoencarnacion electrochemiluminescentnanostructureddnabiosensorforsarscov2detection