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Perspectives of future lung toxicology studies using human pluripotent stem cells
The absence of in vitro platforms for human pulmonary toxicology studies is becoming an increasingly serious concern. The respiratory system has a dynamic mechanical structure that extends from the airways to the alveolar region. In addition, the epithelial, endothelial, stromal, and immune cells ar...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720162/ https://www.ncbi.nlm.nih.gov/pubmed/34973109 http://dx.doi.org/10.1007/s00204-021-03188-9 |
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author | Masui, Atsushi Hirai, Toyohiro Gotoh, Shimpei |
author_facet | Masui, Atsushi Hirai, Toyohiro Gotoh, Shimpei |
author_sort | Masui, Atsushi |
collection | PubMed |
description | The absence of in vitro platforms for human pulmonary toxicology studies is becoming an increasingly serious concern. The respiratory system has a dynamic mechanical structure that extends from the airways to the alveolar region. In addition, the epithelial, endothelial, stromal, and immune cells are highly organized in each region and interact with each other to function synergistically. These cells of varied lineage, particularly epithelial cells, have been difficult to use for long-term culture in vitro, thus limiting the development of useful experimental tools. This limitation has set a large distance between the bench and the bedside for analyzing the pathogenic mechanisms, the efficacy of candidate therapeutic agents, and the toxicity of compounds. Several researchers have proposed solutions to these problems by reporting on methods for generating human lung epithelial cells derived from pluripotent stem cells (PSCs). Moreover, the use of organoid culture, organ-on-a-chip, and material-based techniques have enabled the maintenance of functional PSC-derived lung epithelial cells as well as primary cells. The aforementioned technological advances have facilitated the in vitro recapitulation of genetic lung diseases and the detection of ameliorating or worsening effects of genetic and chemical interventions, thus indicating the future possibility of more sophisticated preclinical compound assessments in vitro. In this review, we will update the recent advances in lung cell culture methods, principally focusing on human PSC-derived lung epithelial organoid culture systems with the hope of their future application in toxicology studies. |
format | Online Article Text |
id | pubmed-8720162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-87201622022-01-03 Perspectives of future lung toxicology studies using human pluripotent stem cells Masui, Atsushi Hirai, Toyohiro Gotoh, Shimpei Arch Toxicol Review Article The absence of in vitro platforms for human pulmonary toxicology studies is becoming an increasingly serious concern. The respiratory system has a dynamic mechanical structure that extends from the airways to the alveolar region. In addition, the epithelial, endothelial, stromal, and immune cells are highly organized in each region and interact with each other to function synergistically. These cells of varied lineage, particularly epithelial cells, have been difficult to use for long-term culture in vitro, thus limiting the development of useful experimental tools. This limitation has set a large distance between the bench and the bedside for analyzing the pathogenic mechanisms, the efficacy of candidate therapeutic agents, and the toxicity of compounds. Several researchers have proposed solutions to these problems by reporting on methods for generating human lung epithelial cells derived from pluripotent stem cells (PSCs). Moreover, the use of organoid culture, organ-on-a-chip, and material-based techniques have enabled the maintenance of functional PSC-derived lung epithelial cells as well as primary cells. The aforementioned technological advances have facilitated the in vitro recapitulation of genetic lung diseases and the detection of ameliorating or worsening effects of genetic and chemical interventions, thus indicating the future possibility of more sophisticated preclinical compound assessments in vitro. In this review, we will update the recent advances in lung cell culture methods, principally focusing on human PSC-derived lung epithelial organoid culture systems with the hope of their future application in toxicology studies. Springer Berlin Heidelberg 2022-01-01 2022 /pmc/articles/PMC8720162/ /pubmed/34973109 http://dx.doi.org/10.1007/s00204-021-03188-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Article Masui, Atsushi Hirai, Toyohiro Gotoh, Shimpei Perspectives of future lung toxicology studies using human pluripotent stem cells |
title | Perspectives of future lung toxicology studies using human pluripotent stem cells |
title_full | Perspectives of future lung toxicology studies using human pluripotent stem cells |
title_fullStr | Perspectives of future lung toxicology studies using human pluripotent stem cells |
title_full_unstemmed | Perspectives of future lung toxicology studies using human pluripotent stem cells |
title_short | Perspectives of future lung toxicology studies using human pluripotent stem cells |
title_sort | perspectives of future lung toxicology studies using human pluripotent stem cells |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720162/ https://www.ncbi.nlm.nih.gov/pubmed/34973109 http://dx.doi.org/10.1007/s00204-021-03188-9 |
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