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Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier
Miniaturization of biomedical and chemical research areas is performed using microfluidic techniques. Droplet-based microfluidic applications are of high interest for various applications, e.g., high-throughput screening assays. Many of them are based on simple water-in-oil (w/o) or oil-in-water (o/...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720838/ https://www.ncbi.nlm.nih.gov/pubmed/35004199 http://dx.doi.org/10.1016/j.mex.2021.101565 |
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author | Brinkmann, Stephan Oberpaul, Markus Glaeser, Jens Schäberle, Till F. |
author_facet | Brinkmann, Stephan Oberpaul, Markus Glaeser, Jens Schäberle, Till F. |
author_sort | Brinkmann, Stephan |
collection | PubMed |
description | Miniaturization of biomedical and chemical research areas is performed using microfluidic techniques. Droplet-based microfluidic applications are of high interest for various applications, e.g., high-throughput screening assays. Many of them are based on simple water-in-oil (w/o) or oil-in-water (o/w) emulsions that are easily to produce. More complex assays based on separate compartments require the use of multiple emulsions, such as water-in-oil-in-water (w/o/w) or oil-in-water-in-oil (o/w/o) emulsions. In this study an easy, fast to establish method to generate agarose-solidified (w/w/o) double emulsions with ∼55 µm in diameter, in which both agarose-phases are not separated by a surfactant stabilized oil is described. An off-chip emulsion-breaking and washing step of the inner agarose droplets based on density gradient centrifugation was designed, offering new possibilities for high-throughput assays on picoliter scale. In brief, this paper reports: • the protocol to generate agarose-solidified (w/w/o) double emulsions non-seperated by surfactant stabilized oil; • an off-chip washing protocol of agarose-solidified emulsions based on density gradient centrifugation. |
format | Online Article Text |
id | pubmed-8720838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-87208382022-01-07 Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier Brinkmann, Stephan Oberpaul, Markus Glaeser, Jens Schäberle, Till F. MethodsX Method Article Miniaturization of biomedical and chemical research areas is performed using microfluidic techniques. Droplet-based microfluidic applications are of high interest for various applications, e.g., high-throughput screening assays. Many of them are based on simple water-in-oil (w/o) or oil-in-water (o/w) emulsions that are easily to produce. More complex assays based on separate compartments require the use of multiple emulsions, such as water-in-oil-in-water (w/o/w) or oil-in-water-in-oil (o/w/o) emulsions. In this study an easy, fast to establish method to generate agarose-solidified (w/w/o) double emulsions with ∼55 µm in diameter, in which both agarose-phases are not separated by a surfactant stabilized oil is described. An off-chip emulsion-breaking and washing step of the inner agarose droplets based on density gradient centrifugation was designed, offering new possibilities for high-throughput assays on picoliter scale. In brief, this paper reports: • the protocol to generate agarose-solidified (w/w/o) double emulsions non-seperated by surfactant stabilized oil; • an off-chip washing protocol of agarose-solidified emulsions based on density gradient centrifugation. Elsevier 2021-11-02 /pmc/articles/PMC8720838/ /pubmed/35004199 http://dx.doi.org/10.1016/j.mex.2021.101565 Text en © 2021 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Method Article Brinkmann, Stephan Oberpaul, Markus Glaeser, Jens Schäberle, Till F. Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier |
title | Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier |
title_full | Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier |
title_fullStr | Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier |
title_full_unstemmed | Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier |
title_short | Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier |
title_sort | two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier |
topic | Method Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8720838/ https://www.ncbi.nlm.nih.gov/pubmed/35004199 http://dx.doi.org/10.1016/j.mex.2021.101565 |
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